Effects of water temperature changes on serum biochemical indices and gut microbiota of Endemic Fish (Procypris rabaudi) in Jinsha River
CHEN Dan-li1,2, YANG Xiao-ling3, LIU Shuai-shuai1,2, ZHANG Bo1,2, CUI Ge1,2, GAO Han1,2, CHEN Juan1,2
1. College of Environment, Hohai University, Nanjing 210098, China; 2. Key Laboratory of Integrated Regulation and Resource Department on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, China; 3. Scientific Institute of Pearl River WaterResources Protection, Guangzhou 510611, China
Abstract：A controlled experiment was conducted to investigate temperature-induced alterations in serum biochemical indices and gut microbiota of endemic fish (Procypris rabaudi) in Jinsha River, a cascade hydropower development river in southwest China. Three temperature treatments (16℃, 20℃, 24℃) were established with exposure durations of 24h and 10d. Results showed that compared to the ambient temperature group, the serum antioxidant enzyme activity was promoted in the low temperature group after 24h but inhibited after 10d. The decrease in lysozyme (LZM) activity in the low temperature group of 10d (67.12%) was more significant than that of 24h group (52.04%) relative to the ambient temperature group. And the concentrations of glucose (GLU) and CORTISOL were increased significantly in the low temperature groups both 24h and 10d compared with those in the ambient temperature groups. The Chao1index of the low temperature group at 24h were 23.22% and 26.36% lower than those of the ambient temperature and high temperature groups, respectively. The alpha diversity was found to stabilize in the low temperature group after 10d. Compared to 24h, the difference in gut microbiota community structure under different temperature conditions after 10d was smaller, and the low temperatures significantly affected the community composition of the gut microbiota. Co-occurrence network analysis revealed that microbial interactions were simplified and weakened in the low temperature group (24h), whereas prolonged thermal adaptation (10d) was associated with network stabilization. Significant correlations were established between Proteobacteria, Bacteroidetes, and Firmicutes with antioxidant enzymes and LZM activity.
陈丹丽, 杨晓灵, 刘帅帅, 张波, 崔戈, 高寒, 陈娟. 水温变化对金沙江特有鱼类岩原鲤血清生化指标及肠道微生物的影响[J]. 中国环境科学, 2025, 45(4): 2288-2297.
CHEN Dan-li, YANG Xiao-ling, LIU Shuai-shuai, ZHANG Bo, CUI Ge, GAO Han, CHEN Juan. Effects of water temperature changes on serum biochemical indices and gut microbiota of Endemic Fish (Procypris rabaudi) in Jinsha River. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(4): 2288-2297.
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