Enrichment of Anammox under conditions of ammonium, nitrate and acetic acid as substrates

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China Environmental Science ›› 2020, Vol. 40 ›› Issue (10) : 4348-4353.

Water Pollution Control

Enrichment of Anammox under conditions of ammonium, nitrate and acetic acid as substrates

Lü^1,2, Kai^1,2, WANG Kang-zhou^1,2, YAO Xue-wei^1,2, ZHAO Wen-zhao¹, PENG Dang-cong³, LI Hui-juan¹

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Abstract

In order to investigate the characteristic of anammox enrichment process with ammonium, nitrate and acetic acid as substrates, an MBBR with mediums from A²/O system treating municipal wastewater was established to start up anammox process. The results showed that the enrichment of anammox bacteria, with ammonium, nitrate and acetic acid as substrates, combined with denitrification process could be obtained rapidly. TN removal load reached to 920.79mg/(m²·d), and Anammox activity could attain to 3018.19mg/(m²·d) after 130d enrichment. Quantitative PCR results showed that after enrichment, the proportion of Ca. Brocadia increased from 0.89% to 27.80%, which is the dominated community of anammox bacteria. The proportion of Thauera increased from 0.01% to 6.75%, and the proportion of Flavobacterium increased from 0.29% to 11.72%, which are the dominated communities of partial denitrifying bacteria.

Key words

anaerobic ammonium oxidation / biological nitrogen removal / enrichment / moving-bed biofilm reactor

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Lü, Kai, WANG Kang-zhou, YAO Xue-wei, ZHAO Wen-zhao, PENG Dang-cong, LI Hui-juan, HAN Yun. Enrichment of Anammox under conditions of ammonium, nitrate and acetic acid as substrates[J]. China Environmental Science. 2020, 40(10): 4348-4353

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