氨氮和硫酸盐对谷氨酸厌氧生物降解性能的抑制及机理

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Abstract To investigate the effect of ammonia nitrogen and sulfate in the influent on anaerobic degradation of glutamate under the optimal organic loading rate, a lab-scale up-flow anaerobic sludge blanket (UASB) reactor was continuously operated for 1119 days. The results showed that the maximal COD removal efficiency as high as (97.94±0.28)% was obtained at an organic loading rate of 8.0g COD/(L·d). When step-wise increasing the ammonia nitrogen concentration in the influent, the UASB reactor a small fluctuation on glutamate removal efficiency under low ammonia nitrogen concentrations (<1000mg/L), subsequently appeared a distinct decline in the COD removal and methane yield at 2000mg/L, and then the half-inhibitory concentration of ammonia nitrogen exhibited at 4000mg/L. When step-wise increasing sulfate concentration in the influent from 200mg/L to 4000mg/L, methane yield and free sulfide (FS) concentration in the mixture fallen into increasing and decreasing trends, respectively; but the COD removal efficiencies were generally in a high level (>90%). Both the ammonia nitrogen and sulfate in the influent could inhibit the glutamate degradation activity of methane-producing archaea (MPA) through dissociation equilibrium and biological reduction, respectively. Besides, FAN could freely diffuse into microbial cells resulting in proton imbalance and then excessive ATP consumption in the former. In contrast, substrate competitive inhibition was existed as for the reproduction of sulfate-reducing bacteria in the latter.

Key words： free ammonia free sulfide biological activity substrate competitive inhibition methane-producing bacteria

Received: 28 February 2020

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X703

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	CHEN Hong
	HU Ying-bing
	CHEN Chen
	WEI Yan-xiao
	WANG Hong
	HUANG Rong
	YU Guan-long
	YANG Min

Cite this article:

CHEN Hong,HU Ying-bing,CHEN Chen等. Inhibition and process mechanism of ammonia nitrogen and sulfate on anaerobic degradation of glutamate[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(10): 4342-4347.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I10/4342

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