Effects of carbon addition on community structure diversity of CO2-assimilating bacterial in Saline-alkali cultivated land

SU Xin; LUO Hui; YUE Zhong-hui; GUO Ying-lan; JIN Xiao-xia; LU Man

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China Environmental Science ›› 2020, Vol. 40 ›› Issue (10) : 4496-4503.

Effects of carbon addition on community structure diversity of CO₂-assimilating bacterial in Saline-alkali cultivated land

SU Xin, LUO Hui, YUE Zhong-hui, GUO Ying-lan, JIN Xiao-xia, LU Man

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Abstract

The effects of straw(S), biochar(B) and nano-carbon(N) on the structure and diversity of CO₂-assimilating bacteria community in saline-alkali cultivated land were studied with the high-throughput sequencing technology, and the relationship between soil chemical properties and diversity of CO₂-assimilating bacteria community were also analyzed. These three carbon addition sources all reduced the diversity of CO₂-assimilating bacteria community. Chao1index, observed species, Shannon index and PD whole tree of CO₂-assimilating bacteria with B and N addition were higher than those with S addition. These three carbon additions also reduced the species abundance of soil CO₂-assimilating bacteria community. The species abundance of CO₂-assimilating bacteria community in N addition treated samples was higher than that with S and B addition treatments. In terms of community composition and relative abundance, the dominant phylum after these three carbon treatments was Proteobacteria, and the dominant phylum was Gammaproteobacteria. Their relative abundances in N treatment were the highest at 90.38%, and 57.70%, respectively. The results from differences group communities analysis showed that there were significant differences in the community structure of CO₂-assimilating bacteria after the addition of S and N. The results from redundancy analysis showed that the structure of CO₂-assimilating bacteria community was influenced by soil pH, organic carbon, total nitrogen, total phosphorus, available nitrogen and phosphorus. Soil pH and active phosphorus content were the main soil chemical properties affecting the structure of soil CO₂-assimilating bacteria community. Our findings suggested that the addition of S, B or N in saline-alkali cultivated land could inhibit the diversity and species abundance of soil CO₂-assimilating bacteria community, but the addition of N could increase the structure difference of soil CO₂-assimilating bacteria community.

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cbbL gene / carbon addition / CO₂-assimilating bacteria / high-throughput sequencing / saline-alkali cultivated land

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SU Xin, LUO Hui, YUE Zhong-hui, GUO Ying-lan, JIN Xiao-xia, LU Man. Effects of carbon addition on community structure diversity of CO₂-assimilating bacterial in Saline-alkali cultivated land[J]. China Environmental Science. 2020, 40(10): 4496-4503

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