萘厌氧降解菌群的富集及氧化还原介体的强化

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Abstract To reveal the catalytic effect of redox mediators (ROMs) on naphthalene anaerobic degradation, a mesophilic naphthalene anaerobic degrading bacterial consortium was enriched using naphthalene as sole carbon source. The bacterial community structure of inoculated sludge and enriched consortium were analyzed by Illumina MiSeq. The catalytic effect of anthraquinone-2,6-disulfonate (AQDS), anthraquinone-2-sulfonate (AQS) and humic acid on naphthalene degradation was also investigated. Illumina MiSeq sequencing revealed that Pseudomonas, Thauera, and Georgfuchsia were the dominant genera in the enriched consortium. Their relative abundance was 52.4%, 13.8% and 17.6%, respectively. Under the inoculated sludge of 0.23g/L and initial naphthalene of 10mg/L conditions, the naphthalene removal was 64.0% within 9 days by the enriched consortium. The result of enhancement experiment showed that ROMs could enhance the anaerobic degradation of naphthalene. Among them, the enhancement effect of AQDS was higher than other ROMs. At the AQDS of 0.8mmol/L, naphthalene removal reached 92.0% on the 7^th day, which was higher than that of the control by1.2times. In addition, this study also investigated the effect of nitrate on the degradation of naphthalene. The results showed that the naphthalene removal showed a decreasing trend after increasing with nitrate increase from 0.2 to 0.8g/L. When the concentration of NaNO₃ was 0.6g/L, the naphthalene removal reached 91.0% on the 6^th day, which is higher than control by 15.2%. Therefore, appropriate nitrate would enhance the catalytic effect of ROMs on naphthalene degradation.

Key words： naphthalene enriched consortium anaerobic degradation redox mediators nitrate

Received: 20 December 2019

PACS:

X703.5

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	Articles by authors
	BAN Qiao-ying
	YUE Li-feng
	LI Jian-zheng
	YU Min
	ZHANG Li-guo

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BAN Qiao-ying,YUE Li-feng,LI Jian-zheng等. Enrichment of naphthalene anaerobic degrading bacterial consortium and enhancement by redox mediators[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(7): 3150-3155.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I7/3150

[1]	党丽慧,丁润梅,王一帆,等.银川市湖泊、河流沉积物中PAHs污染特征及风险评价[J]. 中国环境科学, 2019,39(5):2202-2209. Dang L H, Ding R M, Wang Y F, et al. Pollution characteristics and risk assessment of PAHs in sediments of lakes and rivers in Yinchuan city[J]. China Environmental Sciencee, 2019,39(5):2202-2209.
[2]	金梦,王梓,黎玉清,等.石化企业周边儿童多环芳烃内暴露负荷的时间变异性[J]. 中国环境科学, 2018,38(5):1943-1950. Jin M, Wang Z, Li Y Q, et al. The research on the temporal variability of the internal exposure levels of polycyclic aromatic hydrocarbons in children around the petrochemical enterprise[J]. China Environmental Sciencee, 2018,38(5):1943-1950.
[3]	张泽宇,王明霞,程永毅,等.反硝化菌群的萘代谢与反硝化偶联机制[J]. 环境科学, 2018,39(5):2438-2445. Zhang Z Y, Wang M X, Cheng Y Y, et al. Investigation of the coupling mechanism between naphthalene degradation and denitrification of a naphthalene degraded bacterial consortium under denitrification[J]. Environmental Science, 2018,39(5):2438-2445.
[4]	黄星云,张泽宇,熊苏雅,等.菲反硝化降解菌群的富集及其群落结构解析[J]. 环境科学学报, 2017,37(11):4314-4321. Huang X Y, Zhang Z Y, Xiong S Y, et al. Enrichment and community structure analysis of phenanthrene degrading bacterial consortium[J]. Acta Scientiae Cirumstantiae, 2017,37(11):4314-4321.
[5]	孙明明,滕应,骆永明.厌氧微生物降解多环芳烃研究进展[J]. 微生物学报, 2012,52(8):931-939. Sun M M, Teng Y, Luo Y M, Progresses in anaerobic biodegradation of polycyclic aromatic hydrocarbons-A review[J]. Acta Microbiologica Sinica, 2012,52(8):931-939.
[6]	Musat F, Galushko A, Jacob J, et al. Anaerobic degradation of naphthalene and 2-methylnaphthalene by strains of marine sulfate-reducing bacteria[J]. Environmental Microbiology, 2009,11:209-219.
[7]	李丽华.聚吡咯固定化介体强化偶氮染料和硝基化合物厌氧生物转化[D]. 大连:大连理工大学, 2008. Li L H. Enhancement of azo dyes and nitro compounds anaerobic biotransformation using a redox mediator immobilized by polypyrrole[D]. Dalian:Dalian University of Technology, 2008.
[8]	康丽,郭建博,李洪奎,等.氧化还原介体催化强化偶氮染料脱色研究进展[J]. 河北工业科技, 2010,27(6):447-450. Kang L, Guo J B, Li H K, et al. Research advance of cataly tic ef fect of redox mediator in azo dye decolorization process[J]. Hebei Journal of Industrial Science and Technology, 2010,27(6):447-450.
[9]	李海波,廉静,郭延凯,等.氧化还原介体催化强化Paracoccus versutus菌株GW1反硝化特性研究[J]. 环境科学, 2012,33(7):2458-2463. Li H B, Lian J, Guo Y K, et al. Biocatalyst of Redox Mediators on the Denitrification by Paracoccus versutus Strain GW1[J]. Envrironmental Science, 2012,33(7):2458-2463.
[10]	Zhang D, Zhang N, Yu X et al. Effect of humins from different sediments on microbial degradation of 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153), and their polyphasic characterization[J]. Rsc Advances, 2017,7(12):6849-6855.
[11]	国家环保局《水和废水监测分析方法》编委会.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2002:27-30. State Environmental Protection Administration. Water and wastewater monitoring and analysis methods[M]. Beijing:China Environmental Science Press, 2002:27-30.
[12]	Ting W T E, Yuan S Y, Wu S D, et al. Biodegradation of phenanthrene and pyrene by Ganoderma lucidum[J]. International Biodeterioration and Biodegradation, 2011,65:238-242.
[13]	Liang L, Song X, Kong J, et al. Anaerobic biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a facultative anaerobe Pseudomonas sp. JP1[J]. Biodegradation, 2014, 25:825-833.
[14]	Lin M, Hu X, Chen W, et al. Biodegradation of phenanthrene by Pseudomonas sp. BZ-3, isolated from crude oil contaminated soil[J]. International Biodeterioration and Biodegradation, 2014,94:176-181.
[15]	Song B, Young L Y, Palleroni N J. Identification of denitrifier strain T I as Thauera arornatica and proposal for emendation of the genus Thauera definition. International Journal of Systematic Bacteriology, 1998,48:889-894.
[16]	黄兴如,张彩文,张瑞杰,等.多环芳烃降解菌的筛选、鉴定及降解特性[J]. 微生物学通报, 2016,43(5):965-973. Huang X R, Zhang C W, Zhang R J, et al. Isolation, identification and degrading properties of PAHs-degrading bacteria[J]. Microbiology China, 2016,43(5):965-973.
[17]	Weelink S A B, van Doesburg W, Saia F T, et al. A strictly anaerobic betaproteobacterium Georgfuchsia toluolica gen. nov., sp. nov. degrades aromatic compounds with Fe(III), Mn(IV) or nitrate as an electron acceptor[J]. FEMS Microbiology Ecology, 2009,70:575-585.
[18]	Salati S, Papa G, Adani F. Perspective on the use of humic acids from biomass as natural surfactants for industrial applications[J]. Biotechnology Advances, 2011,29(6):913-922.
[19]	王竞,吕红.新型介体强化污染物生物还原[M]. 北京:科学出版社, 2013:2. Wang J, Lv H. New mediators enhance biological reduction of pollutants[M]. Beijing:Science Press, 2013:2.
[20]	Dou J F, Liu X, Ding A Z. Anaerobic degradation of naphthalene by the mixed bacteria under nitrate reducing conditions[J]. Journal of Hazardous Materials, 2009,165:325-331.