洛美沙星对水中反硝化过程的影响模拟试验

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Abstract The removal of nitrate in aquatic environment depends on denitrification by microorganisms under anaerobic conditions. It has been found that the presence of antibiotics can affect the activity of denitrifying bacteria and denitrifying enzymes, and then affect denitrification. However, the relationships among these three factors and the potential influencing mechanisms remains unclear in water system. In this study, simulation experiments were carried out to determine the effects of antibiotic on denitrification process, denitrification bacteria and denitrification enzyme activities. In the experiments, lomefloxacin (LOM) was selected as a typical antibiotic and sodium acetate was used as a carbon source. The results showed that the denitrification rates exposed to 0, 1, 10 and 100μg/L LOM were 10.42, 8.83, 8.50 and 6.62mg/(L·d), and the nitrogen removal reached 79.5%, 71.1%, 70.0% and 66.8%, respectively. For 100μg/L LOM exposure, the inhibition rate on denitrifying bacteria was up to 30.5%. This suggested that LOM with a certain concentration could affect the microbial growth processes and denitrifying enzyme activity. Therefore, the inhibition of NO₃^--N transformation during denitrification was observed, which further led to the decrease in denitrification and total nitrogen removal rate. Moreover, the higher the initial LOM concentration, the more significant the inhibition effect (P<0.05). Further results demonstrated that effects of LOM on denitrification mainly functioned in the process of NO₃^--N reduction to NO₂^--N. With increasing initial LOM concentration, the growth rate and maximum increment of bacteria decreased, and the damage to the integrity of microbial cell membrane became worse. The transformation of NO₃^--N and NO₂^--N was related to denitrifying enzyme activity. LOM concentration had a significant influence on nitrate reductase activity. The higher the concentration, the greater the degree of inhibition. However, the activity of nitrite reductase was hardly affected LOM concentration. Although the above simulated experimental conditions did not completely matched with the real water, the results implied that the low concentration of antibiotics in environment water body is likely to affect the denitrification process by inhibiting the microbial population and activity as well as the denitrifying enzyme activity, and consequently reduce the denitrification rate and lead to the accumulation of nitrate pollution.

Key words： lomefloxacin nitrate denitrifying bacteria denitrifying enzyme

Received: 04 December 2019

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X703

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	DENG Lu
	HE Jiang-tao
	ZOU Hua
	CHEN Cui-bai

Cite this article:

DENG Lu,HE Jiang-tao,ZOU Hua等. Simulation experiments on effects of lomefloxacin on denitrification process in water[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(7): 2934-2942.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I7/2934

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