Application of in vitro assays in the biotoxicity detection of landfill leachate and reclaimed water

XU Qian-ru; ZHAO Fei; XIA Na-na; LI Xin-hui; GAO Xu-chao; YANG Yong-chuan; BI Xue-jun

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China Environmental Science ›› 2020, Vol. 40 ›› Issue (9) : 3887-3893.

Water Pollution Control

Application of in vitro assays in the biotoxicity detection of landfill leachate and reclaimed water

XU Qian-ru¹, ZHAO Fei¹, XIA Na-na², LI Xin-hui¹, GAO Xu-chao¹, YANG Yong-chuan¹, BI Xue-jun¹

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Abstract

Main in vitro models and in vitro assays for biotoxicity detection of landfill leachate and reclaimed water, and the application of these models and assays in the evaluation of biotoxicity were summarized. The commonly used in vitro models included human-derived cells, other mammalian cells, and microbial cells. In comparison, results obtained from human-derived cells were more persuasive when extrapolated to humans, which led to more widely application of human-derived cell models. One the other hand, in vitro assays included three aspects: detections of cell toxicity, genotoxicity, and endocrine disturbing effects. Among them, studies on endocrine disturbing effects focused more on estrogenic effects nowadays. Moreover, this paper proposed that the development of three-dimensional in vitro model, combination of in vitro assays with chemical analysis, more comprehensive analyses of endocrine disrupting effects, and establishment of group test system of in vitro assays could be the potential future directions for the development of biotoxicity assays of landfill leachate and reclaimed water.

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in vitro assays / in vitro models / biotoxicity / landfill leachate / reclaimed water

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XU Qian-ru, ZHAO Fei, XIA Na-na, LI Xin-hui, GAO Xu-chao, YANG Yong-chuan, BI Xue-jun. Application of in vitro assays in the biotoxicity detection of landfill leachate and reclaimed water[J]. China Environmental Science. 2020, 40(9): 3887-3893

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