氯和紫外消毒过程中胞外抗性基因的产生特征

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Abstract In this study, the generation of eARGs in different physical forms during wastewater chlorination and ultraviolet (UV) disinfection was investigated, and the correlation with microbial communities was explored. Results indicated that though chlorination decreased the abundance of intracellular ARGs, absorbed eARGs was significantly amplified by (0.7±0.1)log, while the abundance of free eARGs decreased by (0.2±0.1)log. UV disinfection also decreased the abundance of intracellular ARGs, but caused a significant increase of free eARGs ((0.4±0.2)log) and a decrease of absorbed eARGs ((0.3±0.1)log). Post chlorination, the abundance of most phyla increased while Proteobacteria decreased in the absorbed extracellular DNA (a-eDNA), resulting in a increase of the bacterial diversity index from 4.2 to 4.7. Whereas, Proteobacteria increased by 6.6% in the free extracellular DNA (f-eDNA) after chlorination, causing a decrease of the bacterial diversity index from 3.5 to 2.8. Post UV disinfection, the abundance of Proteobacteria in a-eDNA decreased by 36.6%, while the bacterial diversity index increased to 4.8; the abundance of bacteria in f-eDNA changed slightly. The molecular ecological network analysis indicated a wide hosting relationship between ARGs and bacteria genera. tetA, tetX, sulI and sulII were correlated with 17, 15, 15 and 5genera respectively, suggesting changes in potential hosts post disinfection were essential mechanisms of the eARGs generation. This study shows that chlorination and UV disinfection can't eliminate the risk of antibiotic resistance but only change patterns of the risk by inducing the generation of adsorbed and free eARGs.

Key words： chlorination ultraviolet disinfection extracellular antibiotic resistance genes bacterial community molecular ecological network

Received: 19 February 2021

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X172

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	Articles by authors
	FU Shu-sen
	WANG Yi
	WANG Xiao-lin
	WANG Shang-jie
	CHENG Yuan
	BIAN Bo
	ZHANG Sheng-bo
	YUAN Qing-bin

Cite this article:

FU Shu-sen,WANG Yi,WANG Xiao-lin等. Generation of extracellular antibiotic resistance genes during municipal wastewater chlorination and UV disinfection[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(10): 4756-4762.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I10/4756

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