Effect of Fe3+ on nitrogen removal of Anammox in the presence of organic matter

WANG Hai-yue; PENG Ling; MAO Nian-jia; GENG Jin-ju; REN Hong-qiang; XU Ke

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China Environmental Science ›› 2021, Vol. 41 ›› Issue (4) : 1672-1680.

Water Pollution Control

Effect of Fe³⁺ on nitrogen removal of Anammox in the presence of organic matter

WANG Hai-yue, PENG Ling, MAO Nian-jia, GENG Jin-ju, REN Hong-qiang, XU Ke

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Abstract

The tolerance of Anammox to organic matter in the presence of Fe³⁺ (2.24~7.84mg/L) was investigated by running four reactors (R1control, R2 2.24mg/L Fe³⁺, R3 4.48mg/L Fe³⁺, R4 7.84mg/L Fe³⁺). The mechanism was clarified by using 16SrRNA high-throughput sequencing technology and qPCR. The results showed that high NH₄⁺-N and TN removal rates (> 90%) of all four reactors could be achieved at the influent COD concentration of 50 and 100mg/L, and the presence of Fe³⁺ did not show the obvious positive effect. As the influent COD concentration increased to 150 and 200mg/L, the performance of Anammox was inhibited and the positive effect of Fe³⁺ increased. When the influent COD concentration was 200mg/L, the addition of Fe³⁺ (7.84mg/L) increased the NH₄⁺-N and TN removals from 61.3% and 79.8% (R1) to 71.2% and 84.7%, respectively. 16SrRNA high-throughput sequencing results indicated the decrease of Anammox bacteria and the proliferation of denitrifying bacteria in the presence of organic matter. The presence of Fe³⁺ could increase the abundance of Planctomycetes. Fe³⁺ had promoting effects on the abundance of Anammox 16S rRNA and functional gene hzsB by qPCR data analysis.

Key words

Anammox / biological nitrogen removal / COD / expanded granular sludge bed (EGSB) / Fe³⁺

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WANG Hai-yue, PENG Ling, MAO Nian-jia, GENG Jin-ju, REN Hong-qiang, XU Ke. Effect of Fe³⁺ on nitrogen removal of Anammox in the presence of organic matter[J]. China Environmental Science. 2021, 41(4): 1672-1680

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