Effect of chlorine on cell-surface interaction and bacterial adhesion behavior

CAI Yin-nuo; LIU Li; CHEN Guo-wei; ZHONG Shu-ying

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China Environmental Science ›› 2023, Vol. 43 ›› Issue (10) : 5188-5195.

Water Pollution Control

Effect of chlorine on cell-surface interaction and bacterial adhesion behavior

CAI Yin-nuo, LIU Li, CHEN Guo-wei, ZHONG Shu-ying

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Abstract

In this study, disinfectant-shaped bacterial adhesion behaviors on pipes with various materials and roughness were examined to underpin the underlying mechanisms of bacterial surface attachment in drinking water. The interaction between bacteria and rough surfaces was analyzed via XDLVO (extended-Derjaguin-Landau-Verwey-Overbeek) theory based on surface thermodynamics. The results showed that chlorine (≤ 1.0mg/L) significantly promoted bacterial adhesion on pipe surfaces (with a 4~6times increment compared to the situation with no chlorine). Besides, bacterial cells tended to colonize the plastic surfaces and increasing surface roughness can further promote surface adhesion. The XDLVO-based theoretical analysis showed that 1.0mg/L chlorine increases the acid-base and van der Waals interaction terms between bacteria and pipe surfaces, which encourages surface adhesion behaviors. Increasing surface roughness further intensifies cell-surface interactions. Compared with stainless steel pipes, polyvinyl chloride and polyethylene surfaces can elevate the interaction energy with bacterial cells, particularly at 1.0mg/L chlorine, which further contributes to bacterial surface attachment and aggregation.

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disinfectant / drinking water / surface roughness / water supply pipe material / XDLVO theory

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CAI Yin-nuo, LIU Li, CHEN Guo-wei, ZHONG Shu-ying. Effect of chlorine on cell-surface interaction and bacterial adhesion behavior[J]. China Environmental Science. 2023, 43(10): 5188-5195

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