热水解污泥酸化液碳氮磷对PHA合成的影响

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Abstract Based on the domestication of the acid liquor of fermented thermal-hydrolyzed sludge, the mixed microbial cultures for producing PHA were obtained and its characteristics were explored. The influence mechanism of feeding method and composition (i.e., N, P, non-VFAs) of the acid liquor on the PHA yield and microbial activity were also investigated. Specifically, the predominant Brachymonas accounted for 45% of the unique mixed microbial cultures that yield PHA. The PHA productivity was improved from 22wt% to 25wt% by limiting the concentration of NH+ 4-N in the acid liquor. By limiting the concentration of non-VFAs, not only the PHA productivity was improved 27% but also the production efficiency was improved 25%. Microbial activity was not effected significantly by non-VFAs、NH₄⁺-N and PO₄^3--P in acid liquor while was obviously restrained by volatile fatty acids (VFAs) with high concentration. To mitigate inhibition of VFAs and improve the PHA yield, intermittent feeding (5times) strategy was used. The PHA yield was effectively improved to 34wt% compared with 28wt% in disposable feeding. Furthermore, the PHA yield was improved by increasing the VFAs proportion in the acid liquor and optimizing the feeding method. The production of PHA by microorganism from fermented thermal-hydrolyzed sludge will be great promise in future industrialization.

Key words： mixed microbial cultures thermal-hydrolyzed sludge polyhydroxyalkanoates (PHA) volatile fatty acids (VFAs)

Received: 24 February 2023

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X705

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	Articles by authors
	HE Yun
	LI Kui-xiao
	JIANG Da-wei
	CHANG Jing
	HAO Shan
	WANG Gang
	WANG Jia-wei

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HE Yun,LI Kui-xiao,JIANG Da-wei等. Effects of carbon, nitrogen and phosphorus in fermented thermal-hydrolyzed sludge on polyhydroxyalkanoates production[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(10): 5181-5187.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I10/5181

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