Soil-microbe characterization and interaction in alpine degraded grassland in Maqu county

WANG Min; LIU Min-xia; WANG Qian-yue; MIAO Le-le; YANG Chun-liang; XIAOYin-di

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China Environmental Science ›› 2023, Vol. 43 ›› Issue (12) : 6482-6489.

Soil Pollution Control

Soil-microbe characterization and interaction in alpine degraded grassland in Maqu county

WANG Min, LIU Min-xia, WANG Qian-yue, MIAO Le-le, YANG Chun-liang, XIAOYin-di

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Abstract

In this paper, four kinds of alpine grassland with different degradation levels in Maqu county, Gannan Tibetan Autonomous Prefecture, Gansu Province, were used as the research objects, and soil physicochemical factors and soil microbial community characteristics were measured and analyzed. The results showed that the soil water content, soil organic carbon, total nitrogen, and total phosphorus decreased significantly (P<0.05) with increased grassland degradation. In contrast, the trends of soil pH and soil electrical conductivity were opposite. Through high-throughput sequencing, we analyzed and obtained the numbers of bacterial and fungal OTUs in no, light, moderate, and severely degraded grassland were 1927, 2011, 2040, 1798 and 1492, 1511, 1383, 1466; the numbers of bacterial and fungal OTUs specific to no, light, moderate and severely degraded grassland were 588, 513, 691, 409 and 116, 112, 73, 79. The number of soil microbial community bacteria dominated significantly, and the diversity decreased with the degradation of grassland as a whole. The relative abundance of the dominant group of soil bacteria, Proteobacteria, reached a maximum of 45% in the moderately degraded grassland; the relative abundance of the dominant group of soil fungi, Ascomycota, reached a maximum of 89% in the non-degraded grassland. Through RDA analysis, it was obtained that soil electrical conductivity, organic carbon, water content, total nitrogen, total phosphorus and pH were the main influence factors of soil bacteria, and soil total phosphorus, total nitrogen, and organic carbon were the main influence factors of soil fungi.

Key words

degraded grassland / high-throughput sequencing technology / Maqu county / redundancy analysis / soil microorganisms

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WANG Min, LIU Min-xia, WANG Qian-yue, MIAO Le-le, YANG Chun-liang, XIAOYin-di. Soil-microbe characterization and interaction in alpine degraded grassland in Maqu county[J]. China Environmental Science. 2023, 43(12): 6482-6489

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