耦合内源反硝化实现厌氧氨氧化工艺深度脱氮

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Abstract To achieve advanced nitrogen removal of anaerobic ammonia oxidation (anammox) process, endogenous denitrification was combined with anammox by adding sodium acetate (100mg COD/L) in the anaerobic stage of sequencing batch reactor. The nitrogen removal performance and the variations of microbial community structure in the reactor were also investigated during operation. Results showed a stable and high nitrogen removal efficiency of anammox process was achieved by coupled with endogenous denitrification. The total inorganic nitrogen (TIN) removal efficiency was increased from 79.07% ±2.63% to 97.00% ±1.35% and the effluent TIN concentration was reduced to (3.30 ±1.49)mg/L. Typical cycle data showed that the internal carbon sources such as poly-β-hydroxyalkanoates synthesized in the anaerobic stage could provide electron donors for subsequent endogenous denitrification, meanwhile the reaction rate of anammox was not affected. The nitrogen in the system, calculated by mass balance analysis, was mainly removed by anammox (P_AMX:P_EDN=98.27% :1.73%). Further, the sodium acetate addition stimulated the proliferation of denitrifying bacteria (i.e. Thauera) in the system. However, with the abudance increase of Denitratosoma capable of endogenous denitrifying capacity, the abundance of Anammox bacteria (i.e. Candidatus Brocadia) was restored, and therefore the advanced nitrogen removal of anammox system was realized.

Key words： endogenous denitrification Anammox SBR advanced nitrogen removal microbial community structure

Received: 27 August 2022

PACS:

X703.1

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	LIU Wen-long
	WANG Jia-ming
	LI Jun

Cite this article:

LIU Wen-long,WANG Jia-ming,LI Jun. Achieving advanced nitrogen removal via anammox coupled with endogenous denitrification[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1735-1743.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I4/1735

[1]	Ma B, Wang S, Cao S, et al. Biological nitrogen removal from sewage via anammox:Recent advances[J]. Bioresource Technology, 2016, 200:981-990.
[2]	Strous M, Heijnen J J, Kuenen J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology and Biotechnology, 1998,50(5):589-596.
[3]	Takekawa M, Park G, Soda S, et al. Simultaneous anammox and denitrification (SAD) process in sequencing batch reactors[J]. Bioresource Technology, 2014,174:159-166.
[4]	Du R, Peng Y, Cao S, et al. Advanced nitrogen removal with simultaneous Anammox and denitrification in sequencing batch reactor[J]. Bioresource Technology, 2014,162:316-322.
[5]	Ge C H, Sun N, Kang Q, et al. Bacterial community evolutions driven by organic matter and powder activated carbon in simultaneous anammox and denitrification (SAD) process[J]. Bioresource Technology, 2018,251:13-21.
[6]	Wang B, Gong X, Peng Y. Simultaneous anammox-denitrification process and its emerging extensions[J]. Chemical Engineering Journal, 2021,415:128380.
[7]	Kumar M, Lin J G. Co-existence of anammox and denitrification for simultaneous nitrogen and carbon removal strategies and issues[J]. Journal of Hazardous Materials, 2010,178(1-3):1-9.
[8]	Zeng R J, Yuan Z G, Keller J. Enrichment of denitrifying glycogen-accumulating organisms in anaerobic/anoxic activated sludge system[J]. Biotechnology and Bioengineering, 2003,81(4):397-404.
[9]	Ji J T, Peng Y Z, Mai W K, et al. Achieving advanced nitrogen removal from low C/N wastewater by combining endogenous partial denitrification with anammox in mainstream treatment[J]. Bioresource Technology, 2018,270:570-579.
[10]	Santorio S, Fra-Vazquez A, del Rio AV, et al. Potential of endogenous PHA as electron donor for denitrification[J]. Science of the Total Environment, 2019,695:133747.
[11]	Wu P, Chen J J, Garlapati V K, et al. Novel insights into Anammox-based processes:A critical review[J]. Chemical Engineering Journal, 2022,444:136534.
[12]	Feng Y, Luo S P, Zhang Y X, et al. Enhanced nutrient removal from mainstream sewage via denitrifying dephosphatation, endogenous denitrification and anammox in a novel continuous flow process[J]. Bioresource Technology, 2022,351:127003.
[13]	张小玲,张萌,陈紫薇,等.内碳源短程反硝化启动及EPD-ANAMMOX耦合工艺性能[J]. 中国环境科学, 2022,42(2):601-611. Zhang X L, Zhang M, Chen Z W, et al. Start-up of endogenous partial denitrification and performance of EPD-ANAMMOX coupling process[J]. China Environmental Science, 2022,42(2):601-611.
[14]	Lu W K, Ma B, Wang Q Q, et al. Feasibility of achieving advanced nitrogen removal via endogenous denitratation/anammox[J]. Bioresource Technology, 2021,325:124666.
[15]	Zeng R J, Yuan Z, Keller J. Model-based analysis of anaerobic acetate uptake by a mixed culture of polyphosphate-accumulating and glycogen-accumulating organisms[J]. Biotechnology and Bioengineering, 2003,83(3):293-302.
[16]	Oehmen A, Yuan Z, Blackall L, et al. Comparison of acetate and propionate uptake by polyphosphate accumulating organisms and glycogen accumulating organisms[J]. Biotechnology and Bioengineering, 2005,91(2):162-168.
[17]	Cui Y C, Gao J F, Zhang D, et al. Responses of performance, antibiotic resistance genes and bacterial communities of partial nitrification system to polyamide microplastics[J]. Bioresource Technology, 2021,341:125767.
[18]	宋成康,王亚宜,韩海成,等.温度降低对厌氧氨氧化脱氮效能及污泥胞外聚合物的影响[J]. 中国环境科学, 2016,36(7):2006-2013. Song C K, Wang Y Y, Han H C, et al. Effect of decreasing temperature on the performance and extracellular polymer substance of anaerobic ammonia oxidation sludge[J]. China Environmental Science, 2016, 36(7):2006-2013.
[19]	Li J L, Peng Y Z, Li X Y, et al. Improving efficiency and stability of anammox through sequentially coupling nitritation and denitritation in a single-stage bioreactor[J]. Environmental Science and Technology, 2020,54:10859-10867.
[20]	Ma X, Wang Y. Anammox bacteria exhibit capacity to withstand long-term starvation stress:A proteomic-based investigation of survival mechanisms[J]. Chemosphere, 2018,211:952-961.
[21]	Strous M, Heijnen J J, Kuenen J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology and Biotechnology, 1998,50(5):589-596.
[22]	黄孝肖,陈重军,张蕊,等.厌氧氨氧化与反硝化耦合反应研究进展[J]. 应用生态学报, 2012,23(3):849-856. Huang X X, Chen C J, Zhang R, et al. Research progress in anammox-denitrification coupling process[J]. Chinese Journal of Applied Ecology, 2012,23(3):849-856.
[23]	孙艳波,周少奇,李伙生,等.ANAMMOX与反硝化协同脱氮反应器启动及有机负荷对其运行性能的影响[J]. 化工学报, 2009,60(10):2596-2602. Sun Y B, Zhou S Q, Li H S, et al. Start-up of ANAMMOX-denitrification reactor and effect of organic loading on its performance of synergistic interaction[J]. CIESC Journal, 2009,60(10):2596-2602.
[24]	Li J, Qiang Z, Yu D, et al. Performance and microbial community of simultaneous anammox and denitrification (SAD) process in a sequencing batch reactor[J]. Bioresource Technology, 2016,218:1064-1072.
[25]	Mino T, Liu W T, Kurisu F, et al. Modeling glycogen-storage and denitrification Capability of microorganisms in enhanced biological phosphate removal processes[J]. Water Science and Technology, 1995, 31(2):25-34.
[26]	Carta F, Beun J J, Van Loosdrecht M C M, et al. Simultaneous storage and degradation of PHB and glycogen in activated sludge cultures[J]. Water Research, 2001,35(11):2693-2701.
[27]	杨艳平,赵辰,张纯纯,等.聚糖菌快速富集方法的建立及群落特性分析[J]. 环境工程学报, 2021,15(5):1792-1802. Yang Y P, Zhao C, Zhang C C, et al. Rapid enrichment of glycogen accumulating organisms and analysis of community characteristics[J]. Chinese Journal of Environmental Engineering, 2021,15(5):1792-1802.
[28]	李忠明,王淑莹,苗蕾,等.单级SBR厌氧/好氧/缺氧处理中期垃圾渗滤液深度脱氮[J]. 化工学报, 2015,66(2):746-752. Li Z M, Wang S Y, Miao L, et al. Nitrogen removal from medium-age landfill leachate via anaerobic/aerobic/anoxic process in SBR[J]. CIESC Journal, 2015,66(2):746-752.
[29]	Gonzalez-Martinez A, Osorio F, Morillo J A, et al. Comparison of bacterial diversity in full scale anammox bioreactors operated under different conditions[J]. Biotechnology Progress, 2015,31(6):1464-1472.
[30]	Bhattacharjee A S, Wu S, Lawson C E, et al. Whole-community metagenomics in two different anammox configurations:process performance and community structure[J]. Environmental Science & Technology, 2017,51(8):4317-4327.
[31]	Feng C J, Lotti T, Lin Y M, et al. Extracellular polymeric substances extraction and recovery from anammox granules:Evaluation of methods and protocol development[J]. Chemical Engineering Journal, 2019,374:112-122.
[32]	Kuenen J G. Anammox bacteria:from discovery to application[J]. Nature Reviews Microbiology, 2008,6(4):320-326.
[33]	Zhang Z, Liu S. Insight into the overconsumption of ammonium by anammox consortia under anaerobic conditions[J]. Journal of Applied Microbiology, 2014,117(6):1830-1838.
[34]	Coata M C M S, Carvalho L, Leal C D, et al. Impact of inocula and operating conditions on the microbial community structure of two anammox reactors[J]. Environmental Technology, 2014,35(14):1811-1822.
[35]	Cao S B, Rui D, Li B K, et al. High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating high-strength wastewater[J]. Applied microbiology and biotechnology, 2020,104(7):3205.
[36]	Wang Z Z, Ji Y, Yan L, et al. Simultaneous anammox and denitrification process shifted from the anammox process in response to C/N ratios:Performance, sludge granulation, and microbial community[J]. Journal of Bioscience and Bioengineering, 2020, 130(3):319-326.
[37]	Zhang X, Zhang N, Chen Z, et al. Long-term impact of sulfate on an autotrophic nitrogen removal system integrated partial nitrification, anammox and endogenous denitrification (PAED)[J]. Chemosphere, 2019,235:336-343.
[38]	Ma B, Xu X X, Wei Y, et al. Recent advances in controlling denitritation for achieving denitratation/anammox in mainstream wastewater treatment plants[J]. Bioresource Technology, 2020,299:122697.
[39]	Chu Z R, Wang K, Li X K, et al. Microbial characterization of aggregates within a one-stage nitritation-anammox system using high-throughput amplicon sequencing[J]. Chemical Engineering Journal, 2015,262:41-48.