|
|
Partial nitrification start-up study running alternately between sequential batch and continuous flow |
LI Dong, MAO Zhong-xin, LI Ming-run, WANG Qin-yuan, HU Li-jun |
Beijing Key Laboratory of Water Quality Science and Water Environment Restoration Engineering, Beijing University of Technology, 100124, China |
|
|
Abstract Three groups of reactors of the same specification, respectively referred to as R1(operation mode of continuous flow with low dissolved oxygen and low substrate), R2(operation mode of SBR), R3(alternate operation mode of continuous flow and SBR) were set up to investigate the nitrite nitrogen accumulation, pollutant removal, sludge characteristics, and EPS secretion during the operation period. The start-up time for partial nitrification in the R1was 28days, and the NAR was maintained at 49.6%, with a gradual decrease in ammonia removal and nitrite concentration. R2 achieved partial nitrification in 12days, and the NAR was maintained at 95.6%, but it was reduced to 82.6% due to a decrease in bacterial activity by prolonged FA inhibition. It took 18days for R3 to achieve partial nitrification, then R3 was operated for 90d, with a NAR of 92.3% in the SBR stage and 90.2% in the continuous flow stage. The ratio of NO2--N/ NH4+-N in the effluent of R3was basically between 1.10and 1.22, and the sludge had a relatively dense structure, the SVI was between the optimal threshold 70and 100mL/g, the EPS content reached 54.63mg/gVSS, the SAOR and SNPR were 6.7 and 2.5mgN/(gMLVSS⋅h), respectively. The above research indicates that the alternate operation strategy of sequential batch and continuous flow can effectively elute NOB while ensuring functional bacteria such as AOB, achieving stable accumulation of nitrite.
|
Received: 09 December 2022
|
|
|
|
|
[1] |
Zaman M, Kim M, Nakhla G. Simultaneous partial nitrification and denitrifying phosphorus removal (PNDPR) in a sequencing batch reactor process operated at low DO and high SRT for carbon and energy reduction [J]. Chemical Engineering Journal, 2021,425: 131881.
|
[2] |
Wang L, Qiu S, Guo J, et al. Light Irradiation Enables Rapid Start-Up of Nitritation through Suppressingnxr Gene Expression and Stimulating Ammonia-Oxidizing Bacteria [J]. Environmental Science & Technology, 55.19(2021):13297-13305.
|
[3] |
Regmi P, Miller M W, Holgate B, et al. Control of aeration, aerobic SRT and COD input for mainstream nitritation/denitritation [J]. Water Research, 2014,57:162-171.
|
[4] |
Hellinga C, Schellen A A J C, Mulder J W, et al. The sharon process: An innovative method for nitrogen removal from ammonium-rich waste water [J]. Water Science and Technology, 1998,37(9):135-142.
|
[5] |
Frølund B, Griebe T, Nielsen P H. Enzymatic activity in the activated-sludge floc matrix [J]. Applied Microbiology and Biotechnology, 1995,43:755-761.
|
[6] |
Liu H, Fang H H P. Extraction of extracellular polymeric substances (EPS) of sludges [J]. Journal of Biotechnology, 2002,95(3):249-256.
|
[7] |
Liu W, Li J, Peng Y. Impact of starvation conditions on the nitrifying performance and sludge properties in SBR system with a limited filamentous bulking state [J]. Science of The Total Environment, 2021,797:148997.
|
[8] |
Wang F, Lu S, Wei Y, et al. Characteristics of aerobic granule and nitrogen and phosphorus removal in a SBR [J]. Journal of Hazardous Materials, 2009,164(2):1223-1227.
|
[9] |
Jahn L, Svardal K, Krampe J. Comparison of aerobic granulation in SBR and continuous-flow plants [J]. Journal of Environmental Management, 2019,231:953-961.
|
[10] |
汪 彪,姚 昕,刘绍根,等.连续流好氧颗粒污泥形成影响因素及应用研究进展 [J]. 工业水处理, 2022,42(4):7-15. Wang B, Yao X, Liu S G, et al. Influencing factors and application of continuous flow aerobic granular sludge [J]. Industrial Water Treatment, 2022,42(4):7-15.
|
[11] |
Graaf A A V D, Bruijn P D, Robertson L A, et al. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor [J]. Microbiology, 1996,142:2187-2196.
|
[12] |
Moussa M S, Lubberding H J, Hooijmans C M, et al. Improved method for determination of ammonia and nitrite oxidation activities in mixed bacterial cultures [J]. Applied Microbiology and Biotechnology, 2003,63(2):217-221.
|
[13] |
Kent T R, Sun Y, An Z, et al. Mechanistic understanding of the NOB suppression by free ammonia inhibition in continuous flow aerobic granulation bioreactors [J]. Environment International, 2019,131: 105005.
|
[14] |
Li X Y, Yang S F. Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge [J]. Water Research, 2007,41(5):1022-1030.
|
[15] |
Yu G, He P, Shao L, et al. Toward understanding the mechanism of improving the production of volatile fatty acids from activated sludge at pH 10.0 [J]. Water Research, 2008,42(18):4637-4644.
|
[16] |
Fr Olund B, Griebe T, Nielsen P H. Enzymatic activity in the activated-sludge floc matrix [J]. Applied Microbiology and Biotechnology, 1995,43(4):755-761.
|
[17] |
Liu H, Fang H H P. Extraction of extracellular polymeric substances (EPS) of sludges [J]. Journal of Biotechnology, 2002,95(3):249-256.
|
[18] |
Liu W, Li J, Peng Y. Impact of starvation conditions on the nitrifying performance and sludge properties in SBR system with a limited filamentous bulking state [J]. Science of The Total Environment, 2021,797:148997.
|
[19] |
Ma B, Zhang S, Zhang L, et al. The feasibility of using a two-stage autotrophic nitrogen removal process to treat sewage [J]. Bioresource Technology, 2011,102(17):8331-8334.
|
[20] |
Bae W, Baek S, Chung J, et al. Optimal operational factors for nitrite accumulation in batch reactors [J]. Biodegradation, 2001,12(5):359-366.
|
[21] |
Wang W, Li D, Li S, et al. Characteristics and formation mechanism of hollow Anammox granular sludge in low-strength ammonia sewage treatment [J]. Chemical Engineering Journal, 2021,421:127766.
|
[22] |
Liu W, Li J, Peng Y. Impact of starvation conditions on the nitrifying performance and sludge properties in SBR system with a limited filamentous bulking state [J]. Science of The Total Environment, 2021,797:148997.
|
[23] |
杨 宏,王泊含,刘宗跃,等.短程硝化污泥工业化培养方法探究 [J]. 能源与环境, 2022,(6):2-5. Yang H, Wang B H, Liu Z Y, et al. Exploration on industrial cultivation method of short-range nitrified sludge [J]. Energy & Environment, 2022,(6):2-5.
|
[24] |
李定昌,王 琦,高景峰,等.不同粒径成熟好氧颗粒污泥EPS的三维荧光光谱特性 [J]. 中国给水排水, 2018,34(7):26-31. Li D C, Wang Q, Gao J F, et al. Three-dimensional fluorescence spectral characteristics of EPS of mature aerobic granular sludge with different particle sizes [J]. China Water & Wastewater, 2018,34(7): 26-31.
|
[25] |
Liu W, Song J, Wang J, et al. Achieving robust nitritation in a modified continuous-flow reactor: From micro-granule cultivation to nitrite-oxidizing bacteria elimination [J]. Journal of Environmental Sciences, 2023,124:117-129.
|
[26] |
Li D, Chen H, Gao X, et al. Establishment and optimization of partial nitrification/anammox/partial nitrification/anammox (PN/A/PN/A) process based on multi-stage ammonia oxidation: Using response surface method as a tool [J]. Bioresource Technology, 2022,361: 127722.
|
[27] |
张 凯,李 军,梁东博,等.IFAS工艺短程硝化过程中功能菌的动力学特性 [J]. 中国环境科学, 2020,40(4):1507-1514. Zhang K, Li J, Liang D B, et al. Kinetic characteristics of functional bacteria during short-range nitrification by IFAS process [J]. China Environmental Science, 2020,40(4):1507-1514.
|
[28] |
Liu W, Song J, Wang J, et al. Achieving robust nitritation in a modified continuous-flow reactor: From micro-granule cultivation to nitrite-oxidizing bacteria elimination [J]. Journal of Environmental Sciences (China), 2023,124(1):117-129.
|
[29] |
Sheng G, Yu H, Li X. Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: A review [J]. Biotechnology Advances, 2010,28(6):882-894.
|
[30] |
Sutherland I W. Biofilm exopolysaccharides: a strong and sticky framework [J]. Microbiology, 2001,147(1):3-9.
|
[31] |
Stoodley P, Cargo R, Rupp C J, et al. Biofilm material properties as related to shear-induced deformation and detachment phenomena [Z]. 2002.
|
|
|
|