乌鲁木齐市河马泉新区土壤微生物多样性及其影响因素分析

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Abstract To examine the diversity and influencing factors of the soil microbial community in Hemaquan New District in Urumqi, the soil samples from different directions of the district were collected for physicochemical properties, metagenomics and environmental factor redundancy analysis. The results showed that the soil was salinization, barren and severe drought, the process of urban construction changed the physicochemical properties in different degrees and also affected the microbial diversity of the soil, in which afforestation and civil engineering had a greater impact on the diversity of bacteria and fungi. At the phylum level, Bacteroidetes(56%~8%), Actinobacteriota(32%~14%), Proteobacteria(22%~10%) were the dominant bacteria, and Ascomycota(82%~30%) and Basidiomycota(41%~2%) were the dominant fungi. At the genus level, RB41, Pontibacter, Bacillus, Adhaeribacter, Nitrososphaeraceae and Antarcticibacterium were the dominant bacteria, and Fusarium, Coprinus, Gibberella, Cladosporium and Alternaria were the dominant fungi. The above dominant flora played a role in adapting to drought, salt and alkali, degrading insoluble substances and participating in chemical cycles in soil. A variety of unusual flora in soil was annotated, including bacteria such as Antarcticibacterium and Cesiribacter, and fungi such as Iodophanus and Neocamarosporiu, which were related to the environment and soil characteristics of the district. The archaeal community structure in different soil samples was similar, and the relative abundance of Crenarchaeota(99%~45%) was the highest, the relative abundance of the Ammonia-oxidizing archaea belonging to Crenarchaeota was high, which had a potentially important role in the nitrogen and carbon cycle of the barren soil. The physicochemical factors affected the microbial community structure in different degrees, especially water content, pH, available nitrogen, total phosphorus and electric conductivity. The dominant species such as Proteobacteria, Acidobacteriota, Firmicutes, Mortierellomycota, Crenarchaeota, Thermoplasmatota, and Euryarchaeota were significantly affected. In conclusion, the physicochemical factors and urban expansion affected the soil microbial diversity in Hemaquan New District, and the study provided a scientific basis for soil quality assessment, land development and ecological protection in the district.

Key words： soil microflora physicochemical factor metagenomics redundancy analysis urban construction

Received: 03 March 2023

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X172

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	Articles by authors
	TURSUN Awagul
	ZHANG Xin-qiang
	YUN Feng-ze
	ZHU Yan-bin
	ZHANG-Lei
	DU Wen-juan
	MA Zheng-hai

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TURSUN Awagul,ZHANG Xin-qiang,YUN Feng-ze等. Analysis of soil microbial diversity and influencing factors in Hemaquan New District in Urumqi[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(S1): 277-287.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/IS1/277

[1] Zhang X, Li Z, Tan Z, et al. Isolation and identification of myxobacteria in the saline-alkaline soils of Akesu in Xinjiang [J].Acta Microbiologica Sinica, 2012,52(2):160–168.
[2] 林婉奇,薛立.基于BIOLOG技术分析氮沉降和降水对土壤微生物功能多样性的影响[J].生态学报, 2020,40(12):4188–4197. Lin W Q, Xue L. Analysis of effects of nitrogen deposition and precipitation on soil microbial function diversity based on BIOLOG technique [J].Acta Ecologica Sinica, 2020,40(12):4188-4197.
[3] 陈慧清,李晓晨,于学峰,等.土壤生态系统微生物多样性技术研究进展[J].地球与环境, 2018,46(2):204–209. Chen H Q, Li X C, Yu X F, et al. A review on technique progresses of microbial diversity in soil ecosystem [J].Earth And Environment, 2018,46(2):204-209.
[4] Grimmn N B, Faeth S H, Golubiewski N E, et al. Global change and the ecology of cities [J].Science, 2008,319:756–760.
[5] 李岩,高丽丽,王云鹏.新时代城市新区风貌塑造规划初探—以乌鲁木齐市河马泉新区为例[J].城镇建设, 2019,10:1. Li Y, Gao L L, Wang Y P. A Preliminary study on the planning of New Urban New Area Style Shaping in the New Era -A case study of Urumqi Hemaquan New District [J].Urban and Rual Studies, 2019,10:1.
[6] 鲍士旦.土壤农化分析[M].北京:中国农业出版社, 2005:17-18. Bao T D. Agrochemical analysis of soil [M].Beijing: China Agriculture Press, 2005:17-18.
[7] Pimm S L, Russell G J, Gittleman J L, et al. The future of biodiversity [J].Science, 1995,269:347–350.
[8] Kaye J P, Groffman M, Grimm N B, et al. A distinct urban biogeochemistry? [J].Trends in Ecology & Evolution, 2006,21: 192–199.
[9] Scharenbroch B C, Lloyd J E, Johnson-Maynard J L. Distinguishing urban soils with physical, chemical, and biological properties [J].Pedobiologia, 2005,49:283–296.
[10] 闫冰,陆晴,夏嵩,等.城市土壤微生物多样性研究进展[J].生物多样性, 2022,30(8):191-204. Yan B, Lu Q, Xia S, et al. An overview of advances in soil microbial diversity of urban environment [J].Biodiversity Science, 2022,30(8): 22186.
[11] 符方艳,陆宏芳.城市化对南亚热带常绿阔叶林土壤生物群落结构的影响[J].生态环境学报, 2015,24:938–946. Fu F Y, Lu H F. Effects of urbanization on soil community structure under subtropical evergreen broad-leaved forests [J].Ecology and Environmental Sciences, 2015,24(6):938-946.
[12] Savard J P L, Clergeaup P, Mennechez G. Biodiversity concepts and urban ecosystems [J].Landscape and Urban Planning, 2000,48:131– 142.
[13] Mckinney M L. Urbanization as a major cause of biotic homogenization [J].Biological Conservation, 2006,127:247–260.
[14] Buczkowski G, Richmond D S. The effect of urbanization on ant abundance and diversity: A temporal examination of factors affecting biodiversity [J].PLoS ONE, 2012,7:e41729.
[15] Madejon E, Burgos P, Lopez R, et al. Soil enzymatic response to addition of heavy metals with organic residues [J].Biology and Fertility of Soils, 2001,34:144–150.
[16] 王改萍,阿依古丽·托乎提,王茹,等.新疆乌尔禾地区盐渍土壤耐盐细菌多样性与群落结构研究[J].微生物学杂志, 2021,41(2):17–26. Wang G P, Ayiguli·T, Wang R, et al. Diversity and community structure of salt-tolerant bacteria in salinized soil in Wuerhe Area in Xinjiang [J].Journal of microbiology, 2021,41(2):17-26.
[17] 杨珊珊,张晓波,陈邬锦,等.新疆三个不同盐碱地区土壤沉积物中细菌多样性分析[J].生物资源, 2021,43(5):453–460. Yang S S, Zhang X B, Chen W J, et al. Analysis of bacterial diversity in soi sediments of three saline alkali areas in Xinjinag [J].Biotic Resources, 2021,43(5):453-460.
[18] 张玲豫,齐雅柯,焦健,等.河西走廊沙地芦苇(Phragmites australis)根际土壤微生物群落多样性[J].中国沙漠, 2021,41(6):1–9. Zhang L Y, QI Y K, Jiao J, et al. Microbial community diversity of reed rhizosphere soil in different sandy land habitats of Hexi Corridor, Gansu, China [J].Journal of Desert Research, 2021,41(6):1-9.
[19] 王诗慧,常顺利,李鑫,等.天山林区土壤真菌多样性及其群落结构[J].生态学报, 2021,41(1):124–134. Wang S H, Chang S L, Li X, et al. Soil fungal diversity and its community structure in Tianshan Forest [J].Acta Ecologica Sinica, 2021,41(1):124-134.
[20] Frey S D, Knorr M, Parrent J L, et al. Chronic nitrogen enrichment affects the structure and function of the soil microbial community in temperate hardwood and pine forests [J].Forest Ecology & Management, 2004,196(1):159–171.
[21] Stone B W, Li J, Koch B J, et al. Nutrients cause consolidation of soil carbon flux to small proportion of bacterial community [J].Nat Commun, 2021,12(1):3381.
[22] Fierer N, Bradford MA, Jackson RB. Toward an ecological classification of soil bacteria [J].Ecology, 2007,88(6):1354–1364.
[23] Foesel B U, Rohde M, Overmann J. Blastocatella fastidiosa gen. nov., sp. nov., isolated from semiarid savanna soil -the first described species of Acidobacteria subdivision 4[J].Syst. Appl. Microbiol., 2013,36(2):82–89.
[24] 林权虹,田地,高菲,等.坝上地区退耕还林还草措施对土壤细菌的影响[J].土壤, 2022,54(2):307–313. Lin Q H, Tian D, Gao F, et al. Effects of returning farmland to forest and grassland on soil bacteria: A case study in Bashang Area, China [J].Soils, 2022,54(2):307–313.
[25] Roiko M, May M, Relich R F. Characterization of Pontibacter altruii, sp. nov., isolated from a human blood culture [J].New Microbes New Infect. 2017,19:71–77.
[26] 戴文魁,方呈祥.两株沙漠细菌Pontibacter korlensis和Pontibacter akesuensis的trans-omics研究[C]//第十六次全国环境微生物学学术研讨会论文集.兰州:中国微生物学会, 2013:38–39. Dai W K, Fang C X. Study on trans-omics of two desert bacteria Pontibacter korlensis and Pontibacter akesuensis [C]//Proceedings of the 16th National Symposium on Environmental Microbiology. Lanzhou: Chinese society for microbiology, 2013:38-39.
[27] 杨盼,翟亚萍,赵祥,等.丛枝菌根真菌和根瘤菌互作对苜蓿根际土壤细菌群落结构的影响及PICRUSt功能预测分析[J].微生物学通报, 2020,47(11):3868–3879. Yang P, Zhai Y P, Zhao X, et al. Effect of interaction between arbuscular mycorrhizal fungi and rhizobium on Medicago sativa rhizosphere soil bacterial community structure and PICRUSt functional prediction [J].Microbiology Chin., 2020,47(11):3868– 3879.
[28] Lee Y M, Baek K, Lee D H, et al. Antarcticibacterium arcticum sp. nov., a bacterium isolated from marine sediment of the Canadian Beaufort Sea [J].Int. J. Syst. Evol Microbiol., 2020,70(4):2912–2917.
[29] Li A Z, Lin L Z, Zhang M X, et al. Antarcticibacterium flavum gen. nov., sp. nov., isolated from marine sediment [J].Int. J. Syst. Evol. Microbiol., 2018,68(1):254–259.
[30] Yml A, Ykj B, Scs C. Complete genome sequence of Antarcticibacterium flavum JB01H24T from an Antarctic marine sediment [J].Marine Genomics, 2019,50.doi:10.1016/j.margen.2019. 100695.
[31] 肖昌松,周培瑾,王大珍.南极地区海底沉积物中低温菌的鉴定与生长温度特征[J].微生物学报, 1990,30(4):233–242. Xiao C S, Zhou P J, Wang D J. Identification and growth temperature characteristics of cryobacteria in seafloor sediments in Antarctica [J].Acta Microbiol., 1990,30(4):233–242.
[32] 李靖宇,杨瑞,段晓敏,等.白芨滩地区不同生物土壤结皮类型对微生物群落结构和组成的影响[J].生态与农村环境学报, 2023, 39(1):97–106. Li J Y, Yang R, Duan X M, et al. Effects of different biological soil crusting types on microbial community structure and composition in Baijitan area [J].Journal of Ecology and Rural Environment, 2023, 39(1):97–106.
[33] 伊帕热·帕尔哈提,祖力胡玛尔·肉孜,田永芝,等.荒漠植物内生菌多样性及其增强农作物抗旱和耐盐性的研究进展[J].生物技术通报, 2022,38(12):88-99. Epari·P, Zulihumaer·R, Tian Y Z, et al. Research progress in diversity of endophytes microbial communities isolated from desert plants and their strengthening effects on drought and salt tolerance in crops [J].Biotechnol Bull, 2022,38(12):88-99.
[34] 任一,韩畅,杨慧,等.城市五种景观植物土壤微生物多样性研究[J].土壤, 2021,53(4):746–754. Ren Y, Han C, Yang H, et al. Study on soil microbial diversity under five urban landscape plants [J].Soils, 2021,53(4):746–754.
[35] Kraft B, Jehmlich N, Larsen M, et al. Oxygen and nitrogen production by an ammonia-oxidizing archaeon [J].Science, 2022,375:97–100.
[36] Martens-Habbena W, Qin W. Archaeal nitrification without oxygen [J].Science, 2022,375:27–28.
[37] Speth D R, Yu F B, Connon S A, et al. Microbial communities of Auka hydrothermal sediments shed light on vent biogeography and the evolutionary history of thermophily [J].ISME J, 2022,16(7):1750– 1764.
[38] Zhang Y, Ji G D, Wu T, et al. Urbanization significantly impacts the connectivity of soil microbes involved in nitrogen dynamics at a watershed scale [J].Environmental Pollution, 2020,258:113708.
[39] Wang H T, Marshall C W, Cheng M Y, et al. Changes in land use driven by urbanization impact nitrogen cycling and the microbial community composition in soils [J].Scientific Reports, 2017,7:44049.