Characteristics of migration and deposition of microorganisms under different seepage conditions in stormwater recharge

WU Yu-hui; YANG Yue-suo; LU Ying; YAN Zi-han; WANG Qian-dan; SHI Min

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China Environmental Science ›› 2025, Vol. 45 ›› Issue (1) : 487-499.

Environmental Microbiology

Characteristics of migration and deposition of microorganisms under different seepage conditions in stormwater recharge

WU Yu-hui^1,2, YANG Yue-suo¹, LU Ying¹, YAN Zi-han³, WANG Qian-dan¹, SHI Min⁴

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Abstract

Based on the unique hydrodynamic and environmental conditions of stormwater artificial recharge, a series of one-dimensional seepage simulation experiments were conducted. The migration and deposition characteristics of clogging microorganisms within porous media under varying seepage conditions, such as different saturation levels, pH environments, ionic strengths, and recharge rates were systematically analyzed and the development of bioclogging within porous media under different seepage conditions was clarified. DLVO theory was used to reveal the dominant forces during microbial migration under different pH environments and ionic strengths. The results showed that the migration of microorganisms slowed down as saturation decreased, pH lowered, ionic strength of the recharge water increased, or recharge flow rate decreased. Consequently, deposition on the medium increased, while interlayer deposition became more uniform, promoting the formation of bioclogging within the medium. Changes in saturation, pH, or ionic strength had minimal effect on microbial migration when saturation was between 60% and 80%, pH was between 7.5 and 8.5, or ionic strength was between 1and 5mmol/L. Under unsaturated conditions, the impact of recharge rate variations on microbial migration and deposition was weakened when the recharge rate was within the range of 0.5to 1mL/min. Under different pH environments and ionic strengths, the dominant forces during microbial migration in saturated and unsaturated media were electrostatic forces and capillary forces, respectively.

Key words

bioclogging / clogging microorganisms / migration and deposition / seepage state / stormwater artifical recharge

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WU Yu-hui, YANG Yue-suo, LU Ying, YAN Zi-han, WANG Qian-dan, SHI Min. Characteristics of migration and deposition of microorganisms under different seepage conditions in stormwater recharge[J]. China Environmental Science. 2025, 45(1): 487-499

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