高负荷活性污泥工艺污泥膨胀成因及控制

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (2046 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract To investigate the causes of sludge bulking during high load operation of activated sludge and the control effect of adding vanillin, this study examined the overall changes in microbial community, filamentous bacteria genus, extracellular polymeric substance secreting bacteria genus, and functional gene clusters involved in exopolysaccharides synthesis when operating under conventional load or high load with or without vanillin addition. The results indicated that after sludge bulking occurred, there was a decrease in the proportion of proteins to polysaccharides in extracellular polymeric substances. This reduction limited the ability of proteins to act as a biological flocculant and resulted in poorer settling performance of the sludge. The causes of sludge bulking during high load operation were attributed to filamentous bacteria dominated by Sphaerotilus and viscous bulking led by Flavobacterium. The addition of vanillin effectively inhibited the proliferation of Sphaerotilus but still allowed for viscous bulking dominated by Thauera and Zoogloea. Metagenomic sequencing analysis revealed that the abundance of alg, eps, and pel functional gene clusters involved in exopolysaccharide synthesis increased by 0.4~0.5 times, 0.8~1.1 times, and 10.3~15.7 times, respectively. These findings suggest that these gene clusters may be potential factors contributing to the substantial increase in exopolysaccharide content observed in bulking sludge.

Key words： sludge bulking high load operation vanillin microbial community exopolysaccharides

Received: 30 July 2024

PACS:

X703

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	AN Ying
	ZHANG Hui-min
	LIU Yun
	CHEN Guang
	TANG Chen
	ZHOU Zhen

Cite this article:

AN Ying,ZHANG Hui-min,LIU Yun等. Causes and control of sludge bulking in high load activated sludge process[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(2): 768-775.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I2/768

[1] Wang H C, Jiang C C, Wang X, et al. Application of internal carbon source from sewage sludge: A vital measure to improve nitrogen removal efficiency of low C/N wastewater [J]. Water, 2021,13(17): 2338.
[2] Cao S B, Lu D, Phua K M, et al. Organics transformation and energy production potential in a high rate A-stage system: A demo-scale study [J]. Bioresource Technology, 2020,295:122300.
[3] 詹巍.基于污水碳源捕获的菌藻共生SBR脱氮及污泥产甲烷发酵效能与机制 [D]. 哈尔滨:哈尔滨工业大学, 2021.Zhan W. Performance and mechanism of bacteria -algae symbiosis SBR nitrogen removal and sludge methanogenic fermentation based on sewage carbon capture [D]. Harbin: Harbin Institute of Technology, 2021.
[4] Rahman A, Meerburg F A, Ravadagundhi S, et al. Bioflocculation management through high-rate contact-stabilization: A promising technology to recover organic carbon from low-strength wastewater [J]. Water Research, 2016,104(1):485-496.
[5] 李亚男,闫冰,郑蕊,等.一体式部分亚硝化-厌氧氨氧化工艺污泥膨胀发生和恢复过程中胞外聚合物变化特征 [J]. 环境科学学报, 2022,42(11):106-116.Li Y N, Yan B, Zheng R, et al. Study on the characteristics of extracellular polymeric substances changes during the sludge bulking occurrence and restoration in one-stage combined partial nitritation and Anammox process [J]. Acta Scientiae Circumstantiae, 2022,42(11): 106-116.
[6] 潘红光,张莹婷,折洋洋,等.城市污水处理过程污泥膨胀识别与抑制综述 [J]. 控制理论与应用, 2023.Pan H G, Zhang Y T, Zhe Y Y, et al. Review on identiﬁcation and suppression of sludge bulking in municipal wastewater treatment process [J]. Control Theory & Applications, 2023.
[7] 黄潇潇.好氧颗粒污泥反应器启动和运行中的膨胀研究 [D]. 重庆:重庆大学, 2022.Huang X X. Study on Bulking during start-up and operation of aerobic granular sludge reactor [D]. Chongqing: Chongqing University, 2022.
[8] 杨阔,林慧敏,陈坦,等.膨胀污泥中主要丝状菌及生物调控措施研究进展 [J]. 应用化工, 2023,52(12):3365-3370.Yang K, Lin H M, Chen T, et al. Research progress on dominant filamentous bacteria and biological control measures in bulking sludge [J]. Applied Chemical Industry, 2023,52(12):3365-3370.
[9] 赵畅,王文昭,徐期勇.群体感应淬灭菌的分离及其膜污染控制性能 [J]. 环境科学, 2016,37(12):4720-4726.Zhao C, Wang W Z, Xu Q Y. Isolation of quorum quenching bacteria and their function for controlling membrane biofouling [J]. Environmental Science, 2016,37(12):4720-4726.
[10] Shi H X, Wang X, Guo J S, et al. A new filamentous bulking control strategy: The role of N-acyl homoserine lactone (AHL)-mediated quorum sensing in filamentous bacteria proliferation within activated sludge [J]. Chemical Engineering Journal, 2022,428:132097.
[11] Shi H X, Wang J, Liu S Y, et al. New insight into filamentous sludge bulking: Potential role of AHL-mediated quorum sensing in deteriorating sludge floc stability and structure [J]. Water Research, 2022,212:118096.
[12] 施洪鑫.AHLs介导的群感效应对丝状菌膨胀的调控机制 [D]. 重庆:重庆大学, 2021.Shi H X. Role of n-acyl homoserine lactone mediated quorum sensing in filamentous bacteria bulking within activated sludge [D]. Chongqing: Chongqing University, 2021.
[13] 陈嘉亮.城市污水高负荷活性污泥工艺碳分离试验研究 [D]. 唐山:华北理工大学, 2020.Chen J L. Experimental research on separating organic carbon from municipal wastewater by high-rate activated sludge system [D]. Tangshan: North China University of Science and Technology, 2020.
[14] 国家环保局.水和废水监测分析方法(第4版) [M]. 北京:中国环境科学出版社, 2002.State Environmental Protection Administration. Water and wastewater monitoring and analysis methods (4th) [M]. Beijing: China Environmental Science Press, 2002.
[15] 郑月.填料对污泥原位侧流减量工艺强化作用研究 [D]. 上海:上海电力大学, 2020.Zhen Y. Inveatigating the effect of packing carriers on in situ side-stream sludge reduction system [D]. Shanghai: Shanghai University of Electric Power, 2020.
[16] Li Z H, Guo Y, Hang Z Y, et al. Simultaneous evaluation of bioactivity and settleability of activated sludge using fractal dimension as an intermediate variable [J]. Water Research, 2020,178:115834.
[17] 章晨.低压光引发疏水改性聚丙烯酰胺的制备与絮凝性能研究 [D]. 重庆:重庆大学, 2022.Zhang C. Study on the preparation and flocculation properties of low pressure light induced hydrophobically modified polyacrylamide [D]. Chongqing: Chongqing University, 2022.
[18] 李冬梅,施周,梅胜,等.絮凝条件对絮体分形结构的影响 [J]. 环境科学, 2006,27(3):488-492.Li D M, Shi Z, Mei S, et al. Effects of flocculation conditions on aggregates fractal structures [J]. Environmental Science, 2006,27(3): 488-492.
[19] 陈浩林,彭轶,安东,等.分段进水多级A/O工艺污泥膨胀控制专家系统 [J]. 中国给水排水, 2021,37(8):153-159.Chen H L, Peng Y, An D, et al. Expert system for sludge bulking control in multi-stage A/O process [J]. China Water & Wastewater, 2021,37(8):153-159.
[20] 李倩.城镇污水脱氮除磷系统污泥膨胀生物学成因及控制措施研究 [D]. 青岛:青岛理工大学, 2015.Li Qian. An study on biological causes and control measures of sludge bulking in urban sewage nitrogen and phosphorus removal system [D]. Qingdao: Qingdao Technology University, 2015.
[21] Li Z H, Guo Y, Hang Z Y, et al. Simultaneous evaluation of bioactivity and settleability of activated sludge using fractal dimension as an intermediate variable [J]. Water Research, 2020,178:115834.
[22] Peng S N, Wang Z Y, Yu P F, et al. Aggregation and construction mechanisms of microbial extracellular polymeric substances with the presence of different multivalent cations: Molecular dynamic simulation and experimental verification [J]. Water Research, 2023, 232:119675.
[23] Huang L, Jin Y, Zhou D, et al. A review of the role of extracellular polymeric substances (EPS) in wastewater treatment systems [J]. International Journal of Environmental Research and Public Health, 2022,19:12191.
[24] An Q, Chen Y, Tang M, et al. The mechanism of extracellular polymeric substances in the formation of activated sludge flocs [J]. Colloids and Surfaces a-Physicochemical and Engineering Aspects, 2023,663:131009.
[25] Li S, Duan G, Xi Y, et al. Insights into the role of extracellular polymeric substances (EPS) in the spread of antibiotic resistance genes [J]. Environmental Pollution, 2024,343:123285.
[26] He Q, Zhang J, Gao S, et al. A comprehensive comparison between non-bulking and bulking aerobic granular sludge in microbial communities [J]. Bioresource Technology, 2019,294:122151.
[27] 许炜怡,黄凯文,刘崇,等.高氮负荷下硫自养反硝化菌群的胞外聚合物和信号分子特征 [J]. 中国环境科学, 2024,44(8):4314-4325.Xu W Y, Huang K W, Liu C, et al. Characterization of extracellular polymer substances and acyl-homoserine lactones of sulfur-based autotrophic denitrification microbial community under high nitrate loading [J]. China Environmental Science, 2024,44(8):4314-4325.
[28] 郝立图,杨晓欢,田端云,等.吸附剂对市政污水浓缩效能的影响 [J]. 中国环境科学, 2024,44(11):6174-6180.Hao L T, Yang X H, Tian D Y, et al. The influence of adsorbents on the concentration efficiency of municipal wastewater [J]. China Environmental Science, 2024,44(11):6174-6180.
[29] Cui H, Xu R, Yu Z, et al. Tank-dependence of the functionality and network differentiation of activated sludge community in a full-scale anaerobic/anoxic/aerobic municipal sewage treatment plant [J]. Frontiers of Environmental Science & Engineering, 2023,17(3):36.
[30] Lu X, Chen C L, Fu L, et al. Social network of filamentous Sphaerotilus during activated sludge bulking: Identifying the roles of signaling molecules and verifying a novel control strategy [J]. Chemical Engineering Journal, 2023,454:140109.
[31] Ferreira R, Amado R, Padrão J, et al. The first sequenced Sphaerotilus natans bacteriophage-Characterization and potential to control its filamentous bacterium host [J]. FEMS Microbiology Ecology, 2021, 97(4):fiab029.
[32] Zhang B, Ning D L, Nostrand J D V, et al. The call for regional design code from the regional discrepancy of microbial communities in activated sludge [J]. Environmental Pollution 2021,273:116487.
[33] Wu X W, Huang J, Lu Z C, et al. Thiothrix eikelboomii interferes oxygen transfer in activated sludge [J]. Water Research, 2019,151: 134-143.
[34] Zhang H, Song S L, Jia Y Y, et al. Stress-responses of activated sludge and anaerobic sulfate-reducing bacteria sludge under long-term ciprofloxacin exposure [J]. Water Research, 2019,164:114964.
[35] Kim H, Kim J, Ahn D. Effects of carbon to nitrogen ratio on the performance and stability of aerobic granular sludge [J]. Environmental Engineering Research, 2021,26(1):114-122.
[36] Wen Q X, Liu S J, Lin X, et al. Rapid recovery of mixed culture polyhydroxyalkanoate production system from EPS bulking using azithromycin [J]. Bioresource Technology, 2022,350:126944.
[37] Qiang J X, Zhou Z, Wang K Q, et al. Coupling ammonia nitrogen adsorption and regeneration unit with a high-load anoxic/aerobic process to achieve rapid and efficient pollutants removal for wastewater treatment [J]. Water Research, 2020,170:115280.
[38] Shi H X, Wang J, Liu S Y, et al. Potential role of AHL-mediated quorum sensing in inducing non-filamentous sludge bulking under high organic loading [J]. Chemical Engineering Journal, 2023,454(4): 140514.
[39] Wan C L, Lee D J, Yang X, et al. Calcium precipitate induced aerobic granulation [J]. Bioresource Technology, 2015,176:32-37.
[40] 田佳乐,刘洋,李嘉雯,等.产胞外多糖嗜热链球菌eps基因簇的表达及生物信息学分析 [J]. 中国食品学报, 2021,21(11):244-251.Tian J L, Liu Y, Li J W, et al. Expression of eps gene cluster in streptococcus thermophilus exopolysaccharide and bioinformatics analysis [J]. Journal of Chinese Institute of Food Science and Technology, 2021,21(11):244-251.
[41] 代安娜尔,乔少婷,孙思霖,等.嗜热链球菌IMAU20246胞外多糖基因簇及其表达分析 [J]. 微生物学报, 2023,63(1):403-418.Dai A, Qiao S T, Sun S L, et al. Identification and expression profiling of exopolysaccharide gene cluster in Streptococcus thermophilus IMAU20246 [J]. Acta Microbiologica Sinica, 2023,63(1):403-418.
[42] 王帅.胞外多糖特性及其对颗粒污泥聚集性能及稳定性的影响机制研究 [D]. 重庆:重庆大学, 2021.Wang S. Characteristics of exopolysaccharide and its impacts mechanism on granular aggregation and stability [D]. Chongqing: Chongqing University, 2021.
[43] Gregory B W, Lindsey S M, Alex O, et al. Pel polysaccharide biosynthesis requires an inner membrane complex comprised of PelD, PelE, PelF, and PelG [J]. Journal of Bacteriology, 2020,202(8): e00684-19.
[44] Whitfield G B, Marmont L S, Bundalovic-torma C, et al. Discovery and characterization of a Gram-positive Pel polysaccharide biosynthetic gene cluster [J]. PLoS Pathogens, 2020,16(4):1008281.