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| Integrated assessment of pollution level in the effluent/seawater mixture based on the biomarker's response in clam Meretrix meretrix |
| LIN Yi-chen, MENG Fan-ping, WAN Ru, DU Yong-xiang, WANG Yue-jie, CUI Hong-wu |
| Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China |
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Abstract Bivalve Meretrix meretrix were exposed for 9days to mixture of Tuandao MSTP effluents and seawater (volume ratio ranged from 1% to 40%, resulted from dilution with natural seawater). Biological effects of contaminant exposure on bivalves were evaluated by measuring nine biochemical parameters including superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase-GPx, glutathione reductase-GR, reduced glutathione-GSH, thiobarbituric acid reactive substances-TBARs, acetylcholinesterase-AChE and metallothioneins-MTs in digestive gland, as well as hemocyte lysosomal membrane stability-LMS. All the biochemical parameters except SOD and CAT had sensitive responses to effluent exposure, Among which four biomarkers, namely LMS, GPx, MTs and AChE, were more effective for indicating effluent pollution because of the significant correlation with concentrations of effluents (P<0.05 or P<0.01). The calculated IBR (integrated biomarker response) index value (0.61~2.65), based on the responses of these biomarkers, increased with the rise of ratio of effluents, and a significant positive correlation between them was observed (P<0.01). The results proved the usefulness of integrated biological effects measurements and IBR index for the assessment of complicated chemical contamination in seawaters receiving MSTPs effluents.
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Received: 23 December 2015
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