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| The succession of propionate-oxidizing bacteria at acidic conditions was analyzed by RT-PCR |
| BAN Qiao-ying1, ZHANG Rui1, ZHANG Li-guo1, LI Jian-zheng2, MEI Zhi1 |
1. College of Environment and Resource, Shanxi University, Taiyuan 030006, China;
2. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China |
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Abstract Succession of propionate-oxidizing bacteria at acidic conditions was investigated in an upflow anaerobic sludge bed (UASB) reactor by quantitative real-time fluorescence polymerase chain reaction (qPCR). The results showed that at least three identified species of propionate-oxidizing bacteria (Pelotomaculum schinkii, P. propionicum, and Smithella propionica) existed in the UASB system. P. schinkii was dominated at pH7.5~7.1 and its quantity was (5.0~5.8)×103 16S rDNA copies per ng DNA, accounting for above 90.6% of the total detectable propionate-oxidizing bacteria. pH decreased from 7.1 to 6.8 resulted in S. propionica was significantly increased. P. schinkii and S. propionica became dominant propionate-oxidizing bacteria and occupied 88.9%. S. propionica accounted for 74.8% in total detectable propionate-oxidizing bacteria at pH 6.5, whereas S. propionica and P. propionicum succeed to dominant propionate-oxidizing bacteria at pH ≤ 6.0, indicating that S. propionica and P. propionicum were more acid resistant. In addition, the total number of propionate-oxidizing bacteria was gradually reduced at pH ≤ 6.0. It might be one of the reasons for propionate accumulation at acidic conditions in anaerobic reactors.
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Received: 13 March 2017
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