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| Effects of bacteria on growth and lipid production of Scenedesmus obliquus cultivated in municipal wastewater |
| HAN Song-fang, JIN Wen-biao, TU Ren-jie, ZHOU Xu, CHEN Hong-yi |
| Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen 518055, China |
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Abstract In the study, EM bacteria and LAS bacteria were found to enhance the growth and lipid production of Scenedesmus obliquus cultivated in municipal wastewater. The results showed that the lipid production of S. obliquus was increased by 36.2% and 21.5% after adding the above-mentioned bacteria, respectively. According to the GC analysis of the lipids, EM bacteria could increase the content of monounsaturated fatty acid in S. obliquus, and thus improving the grade of biodiesel. The analysis of microbial community structure in municipal wastewater showed that the richness and diversity of bacteria in wastewater were increased significantly. In addition, the abundances of β-Proteobacteria、α-Proteobacteria were increased, while the abundance of Bacteroidetes was decreased.
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Received: 22 March 2017
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| [1] |
Chisti Y. Constraints to commercialization of algal fuels[J]. Journal of Biotechnology, 2013,167(3):201-214.
|
| [2] |
Han S F, Jin W B, Tu R J, et al. Biofuel production from microalgae as feedstock:current status and potential[J]. Critical Reviews in Biotechnology, 2015,35(2):255-268.
|
| [3] |
Abomohra A E, Jin W B, Tu R J, et al. Microalgal biomass production as a sustainable feedstock for biodiesel:current status andperspectives[J]. Renewable & Sustainable Energy Reviews, 2016,64(64):596-606.
|
| [4] |
Yang J, Xu M, Zhang M X, et al. Life-cycle analysis on biodiesel production from microalgae:Water footprint and nutrients balance[J]. Bioresource Technology, 2011,102:159-165.
|
| [5] |
Morales-Amaral M d M, Gómez-Serrano C, Acién F G, et al. Production of microalgae using centrate from anaerobic digestion as the nutrient source[J]. Algal Research, 2015,9:297-305.
|
| [6] |
Han S F, Jin W B, Tu R J, et al. Optimization of aeration for biodiesel production by Scenedesmus obliquus grown in municipal wastewater[J]. Bioprocess and Biosystems Engineering, 2016, 39(7):1073-1079.
|
| [7] |
张亚雷,褚华强,周雪飞,等.废水微藻资源化处理原理与技术[M]. 北京:科学出版社, 2015:166-169.
|
| [8] |
巫小丹,阮榕生,王辉,等.菌藻共生系统处理废水研究现状及发展前景[J]. 环境工程, 2014,(3):34-37.
|
| [9] |
王冰,周集体,杨宝灵,等.光合细菌-藻类共固定深度净化污水的研究[J]. 大连民族学院学报, 2014,16(3):249-252.
|
| [10] |
Gonzalez L E, Bashan Y. Increased growth of the microalgae Chlorella vulgaris when coimmobilized and cocultured in alginate beads with the plant-growth-promoting bacterium Azospirillum brasilense[J]. Applied and Environmental Microbiology, 2000,66(4):1527-1531.
|
| [11] |
Mayali X, Doucette G J. Microbial community interactions and population dynamics of an algicidal bacterium active against Karenia brevis (Dinophyceae)[J]. Harmful Algae, 2002,1(3):277-293.
|
| [12] |
Ramsundar P, Guldhe A, Singh P, et al. Assessment of municipal wastewaters at various stages of treatment process as potential growth media for Chlorella sorokiniana under different modes of cultivation[J]. Bioresource Technology, 2017,227:82-92.
|
| [13] |
Ryu B G, Kim E J, Kim H S, et al. Simultaneous treatment of municipal wastewater and biodiesel production by cultivation of Chlorella vulgaris with indigenous wastewater bacteria[J]. Biotechnology and Bioprocess Engineering, 2014,19(2):201-210.
|
| [14] |
Abomohra A E, Wagner M, El-Sheekh M, et al. Lipid and total fatty acid productivity in photoautotrophic fresh water microalgae:screening studies towards biodiesel production[J]. Journal of Applied Phycology, 2013,25(4):931-936.
|
| [15] |
Stainier R Y, Kunisawa R, Mandel M, et al. Purification and properties of unicellular blue-green algae (order Chroococcales)[J]. Bacteriology Reviews, 1971,35(2):171-205.
|
| [16] |
闫韫.布吉河生物修复过程中氮循环功能菌群分布研究[D]. 哈尔滨:哈尔滨工业大学, 2008.
|
| [17] |
丁彬彬.菌藻生物转盘中LAS高效降解菌群的构建与分析[D]. 哈尔滨:哈尔滨工业大学, 2010.
|
| [18] |
孙萃芳.二甲基甲酰胺降解菌的分离筛选及其降解途径的研究[D]. 哈尔滨:哈尔滨工业大学, 2010.
|
| [19] |
Folch J, Lees M, Stanley G H S. A simple method for the isolation and purification of total lipids from animal tissues[J]. Journal of Biological Chemistry, 1957,226:497-509.
|
| [20] |
Kaczmarzyk D, Fulda M. Fatty acid activation in cyanobacteria mediated by acyl-acyl carrier protein synthetase enables fatty acid recycling[J]. Plant Physiology, 2010,152(3):1598-1610.
|
| [21] |
刘林林,黄旭雄,危立坤,等.15株微藻对猪场养殖污水中氮磷的净化及其细胞营养分析[J]. 环境科学学报, 2014,34(8):1986-1994.
|
| [22] |
Vidyashankar S, VenuGopal K S, Swarnalatha G V, et al. Characterization of fatty acids and hydrocarbons of chlorophycean microalgae towards their use as biofuel source[J]. Biomass & Bioenergy, 2015,77:75-91.
|
| [23] |
Song M, Pei H, Hu W, et al. Evaluation of the potential of 10microalgal strains for biodiesel production[J]. Bioresource Technology, 2013,141:245-251.
|
| [24] |
Ma Y, Wang Z, Yu C, et al. Evaluation of the potential of 9Nannochloropsis strains for biodiesel production[J]. Bioresource Technology, 2014,167:503-509.
|
| [25] |
Stansell G R, Gray V M, Sym S D. Microalgal fatty acid composition:implications for biodiesel quality[J]. Journal of Applied Phycology, 2012,24(4):791-801.
|
| [26] |
Hoekman S K, Broch A, Robbins C, et al. Review of biodiesel composition, properties, and specifications[J]. Renewable & Sustainable Energy Reviews, 2012,16:143-169.
|
| [27] |
Zhang T, Shao M F, Ye L. 454Pyrosequencing reveals bacterial diversity of activated sludge from 14sewage treatment plants[J]. ISME Journal, 2012,6(6):1137-1147.
|
| [28] |
Shao K Q, Zhang L, Wang Y P, et al. The responses of the taxa composition of particle-attached bacterial community to the decomposition of Microcystis blooms[J]. Science of the Total Environment, 2014,488-489:236-242.
|
| [29] |
Ramanan R, Kang Z, Kim B, et al. Phycosphere bacterial diversity in green algae reveals an apparent similarity across habitats[J]. Algal Research, 2015,8:140-144.
|
| [30] |
Lee J, Cho D, Ramanan R, et al. Microalgae-associated bacteria play a key role in the flocculation of Chlorella vulgaris[J]. Bioresource Technology, 2013,131:195-201.
|
|
|
|
