异养硝化菌<em>Acinetobacter</em> sp.的分离鉴定及其脱氮特性

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Abstract

Heterotrophic bacterium, the strain JQ1004 exhibiting simultaneous nitrification and aerobic denitrification were isolated from activated sludge and identified as Acinetobacter by 16S rDNA. This study was focused on the characteristics of nitrogen removal under aerobic condition by Acinetobacter sp. JQ1004. Clearly, a significant decrease of ammonium and COD was observed by 33h, with 99.45% and 92.54% removal efficiency respectively while NH₄⁺-N was used as sole N-source and sodium succinate was used as organic carbon source. Besides, while the strain utilized NO₃^--N as nitrogen source, the removal efficiency of NO₃^--N was 84.42% and total 93.11% of COD was decreased during 34h of incubation. It was interesting to note that higher C/N ratio was required in the degradation of nitrate than ammonium with the same initial concentration under aerobic conditions. Based on the description by modified Compertz model for the experimental data, it was found the isolate using ammonium as a sole nitrogen source showed higher rate of converting nitrogen than using nitrate, with 7.93mg/(L·h) and 4.08mg/(L·h) respectively. Response surface methodology (RSM) experiments proved that efficient nitrogen removal and growth of strain JQ1004occurred with succinate as the carbon source, pH 7.33, 31.80℃, and high C/N ratio of 7.76and dissolved oxygen (with shaking speed of 154.54r/min).

Key words： Acinetobacter sp. heterotrophic nitrification-aerobic denitrification response surface methodology (RSM) kinetics

Received: 29 March 2017

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X172

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	Articles by authors
	WANG Xiu-jie
	WANG Wei-qi
	LI Jun
	LI Yun
	ZHANG Yan-zhuo
	SUN Yi-qi
	WANG Si-yu
	BIAN Wei

Cite this article:

WANG Xiu-jie,WANG Wei-qi,LI Jun等. Isolation and identification of a heterotrophic nitrifier, Acinetobacter sp., and its characteristics of nitrogen removal[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(11): 4241-4250.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2017/V37/I11/4241

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