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Variation of bacterial community structure and enzyme activities in reclaimed soil filled with coal gangues along a relamation chronosequence |
HOU Hu-ping, WANG Chen, LI Jin-rong, DING Zhong-yi, ZHANG Shao-liang, HUANG Lei, DONG Jian, MA Jing, YANG Yong-jun |
School of Environment Science & Spatial Informatics, China University of Mining and Technology, Xuzhou 221116, China |
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Abstract In this study,soil samples from the reclaimed land filled with coal gangue (SS) with different reclamation years (2015,2010,and 2001 years) in Xuzhou mining area and from normal farmlands which were not affected by coal mining subsidence (SSC) were collected.The sequence numbers of bacterial community were determined by the Illumina PE250sequencing method,and the vertical structure and time structure were analyzed.The results showed that:(1) Reclamation decreased the number of bacteria species and the community diversity,but the goodness between SS and SSC increased with the age of reclamation.(2)Firmicutes and Proteobacteria dominated in SS at the phylum level,the number of Firmicutes increased after reclamation and Firmicutes also likely transferred from 20~40cm to 0~20cm soil layer.(3)Bacilli predominantly existed in soils at the class level,the number of Bacilli in 0~20cm soil layer was higher in SS than in SSC samples,and the number in 20~40cm soil layers of SS samples decreased with the increase of the reclamation years.(4)Lactobacillales and Bacillales dominated in SS at the order level.Desulfuromonadales played an important role in the remediation of heavy metal pollution and its number in 0~20cm soil layer of SS was 74.81~99.59% less than that in SSC samples.(5) In SS samples,Bacillus,Enterococcus and Streptococcaceae were the dominant family,while Bacillus,Enterococcus and Lactococcus were the dominant genus,and Bacillus sp.JH7,Enterococcus faecium and Lactococcus piscium were the dominant species.All of these in SS samples were less in number than SSC samples especially in 0~20cm soil layer,while the number in 20~40cm soil layer of SS decreased with the increase of the reclamation years.(6) Dehydrogenase activity was negatively correlated with the number of Firmicutes.spp,but was positively correlated with the number of Actinobacteria and the number of Gammaproteobacteria.Also,the type of dominant soil bacteria did not change,but their quantitative structure varied over time.Owing to Firmicutes are suitable for growth in the absence of water and extreme environments,but Proteobacteria is conducive to soil nitrogen and energy cycling.Therefore,the soil quality was improved by adjusting soil bacterial community structure and shorten the recovery period.
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Received: 14 April 2017
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[1] |
董霁红,卞正富,于敏,等.矿区充填复垦土壤重金属分布特征研究[J]. 中国矿业大学学报, 2010,39(3):335-341.
|
[2] |
Poncelet D M, Cavender N, Cutright T J, et al. An assessment of microbial communities associated with surface mining-disturbed overburden[J]. Environmental Monitoring & Assessment, 2014,186(3):1917-1929.
|
[3] |
马守臣,张合兵,王锐,等.煤矸石填埋场土壤微生物学特性的时空变异[J]. 煤炭学报, 2015,40(7):1608-1614.
|
[4] |
徐良骥,黄璨,章如芹,等.煤矸石充填复垦地理化特性与重金属分布特征[J]. 农业工程学报, 2014,30(5):211-219.
|
[5] |
Luna L, Pastorelli R, Bastida F, et al. The combination of quarry restoration strategies in semiarid climate induces different responses in biochemical and microbiological soil properties[J]. Applied Soil Ecology, 2016,107:33-47.
|
[6] |
王友生,侯晓龙,蔡丽平,等.稀土开采对土壤细菌群落组成与多样性的影响[J]. 中国环境科学, 2017,37(8):3089-3095.
|
[7] |
Dangi S R, Stahl P D, Wick A F, et al. Soil microbial community recovery in reclaimed soils on a surface coal mine site[J]. Soil Science Society of America Journal, 2012,76(3):915-924.
|
[8] |
孟会生,洪坚平,杨毅,等.配施磷细菌肥对复垦土壤细菌多样性及磷有效性的影响[J]. 应用生态学报, 2016,27(9):3016-3022.
|
[9] |
刘远,张辉,熊明华,等.气候变化对土壤微生物多样性及其功能的影响[J]. 中国环境科学, 2016,36(12):3793-3799.
|
[10] |
张瑞娟,李华,王爱英,等.不同复垦方式对铝矿废弃地土壤微生物多样性的影响研究[J]. 农业环境科学学报, 2013,32(10):2012-2019.
|
[11] |
Li J, Zheng Y, Yan J, et al. Effects of different regeneration scenarios and fertilizer treatments on soil microbial ecology in reclaimed opencast mining areas on the Loess Plateau, China[J]. PLoS one, 2013,8(5):e63275.
|
[12] |
Zornoza R, Acosta J A, Faz A, et al. Microbial growth and community structure in acid mine soils after addition of different amendments for soil reclamation[J]. Geoderma, 2016,272:64-72.
|
[13] |
樊文华,白中科,李慧峰,等.不同复垦模式及复垦年限对土壤微生物的影响[J]. 农业工程学报, 2011,27(2):330-336.
|
[14] |
贺龙,李艳琴,李彬春,等.矿区不同植被复垦模式对土壤细菌群落结构的影响[J]. 环境科学, 2017,38(2):752-759.
|
[15] |
Rojas C, Gutierrez R M, Bruns M A. Bacterial and eukaryal diversity in soils forming from acid mine drainage precipitates under reclaimed vegetation and biological crusts[J]. Applied Soil Ecology, 2016,105:57-66.
|
[16] |
Li Y, Chen L, Wen H. Changes in the composition and diversity of bacterial communities 13years after soil reclamation of abandoned mine land in eastern China[J]. Ecological Research, 2015,30(2):357-366.
|
[17] |
陈来红,乔光华,董红丽,等.准格尔露天矿区复垦对土壤细菌多样性的影响研究[J]. 干旱区资源与环境, 2012,26(2):119-125.
|
[18] |
李云驹,李耀基,许建初.昆阳磷矿复垦土壤细菌群落特征与理化因子的相关性分析[J]. 环境科学导刊, 2013,32(6):1-5.
|
[19] |
国家环境保护局,国家技术监督局.土壤环境质量标准(GB 15618-1995)[Z]. 1995-07-13.
|
[20] |
Amato K R, Yeoman C J, Kent A, et al. Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes[J]. The ISME Journal, 2013,7(7):1344-1353.
|
[21] |
Schloss P D, Westcott S L, Ryabin T, et al. Introducing mothur:Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities[J]. Applied Environmental Microbiology, 2009,75(23):7537-7541.
|
[22] |
黄郑郑,曹刚,李紫惠,等.XH02菌强化反应器脱氮过程中菌群结构的高通量分析[J]. 中国环境科学, 2017,37(5):1922-1929.
|
[23] |
Lozupone C, Lladser M E, Knights D, et al. UniFrac:an effective distance metric for microbial community comparison[J]. The ISME Journal, 2011,5(2):169-172.
|
[24] |
李媛媛,张怡康.徐州采煤塌陷区复垦土壤的细菌群落多样性[J]. 江苏农业科学, 2014,42(9):312-315.
|
[25] |
钱奎梅,王丽萍,李江.矿区复垦土壤的微生物活性变化[J]. 生态与农村环境学报, 2011,27(6):59-63.
|
[26] |
殷国兰.岷江干旱河谷辐射松人工林土壤微生物和酶活性动态[D]. 雅安:四川农业大学, 2007.
|
[27] |
万忠梅,宋长春.土壤酶活性对生态环境的响应研究进展[J]. 土壤通报, 2009,40(4):951-956.
|
[28] |
冯伟,管涛,王晓宇,等.沼液与化肥配施对冬小麦根际土壤微生物数量和酶活性的影响[J]. 应用生态学报, 2011,22(4):1007-1012.
|
[29] |
张自坤,张宇,黄治军,等.嫁接对铜胁迫下黄瓜根际土壤微生物特性和酶活性的影响[J]. 应用生态学报, 2010,21(9):2317-2322.
|
[30] |
郭星亮,谷洁,陈智学,等.铜川煤矿区重金属污染对土壤微生物群落代谢和酶活性的影响[J]. 应用生态学报, 2012,23(3):798-806.
|
[31] |
李智兰.矿区复垦对土壤养分和酶活性以及微生物数量的影响[J]. 水土保持通报, 2015,32(2):6-13.
|
[32] |
潘晓鸿.苏云金芽胞杆菌等微生物对环境中毒性金属的固定转化作用研究[D]. 福州:福建农林大学, 2014.
|
[33] |
谢剑波.固氮类芽胞杆菌的分离鉴定及比较基因组学研究[D]. 北京:中国农业大学, 2015.
|
[34] |
Kim H J, Eom H J, Park C, et al. Calcium carbonate precipitation by bacillus and sporosarcina strains isolated from concrete and analysis of the bacterial community of concrete[J]. Journal of Microbiology and Biotechnology, 2016,26(3):540-548.
|
[35] |
Maia O B, Duarte R, Silva A M, et al. Evaluation of the components of a commercial probioticing not blobioticmice experimently challenged with Salmonellaenterica sub sp.enterica ser. Typhimurium[J]. Veterinary Microbiology, 2001,79(2):183-189.
|
[36] |
王宇宏,王翔,李晋川,等.安太堡露天矿复垦地不同林龄对土壤酶活性和微生物区系的影响[J]. 山西农业科学, 2016,44(11):1653-1658.
|
[37] |
张丽娟,王海邻,胡斌,等.煤矿塌陷区土壤酶活性与养分分布及相关研究——以焦作韩王庄矿塌陷区为例[J]. 环境科学与管理, 2007,32(1):126-129.
|
|
|
|