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Effects of pure MC-LR and Microcystis aeruginosa crude extracts on photosynthesis of Oryza sativa L |
SHI Yue1,2, JIANG Jin-lin2, DENG Zheng-dong1 |
1. National Defense College of Engineering, PLA University of Science and Technology, Nanjing 210009, China;
2. Key laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China |
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Abstract Microcystins (MCs) and other toxic metabolites, released from cyanobacterial blooms, can be transferred to farmland via irrigation and therefore may have adverse effects on terrestrial crops. In the present study, the net photosynthetic rate, transpiration rate, stomatal conductivity and chlorophyll fluorescence, as well as the ultrastructural features of leaves of rice (Oryza sativa L.) at vegetative stage were investigated under the exposure to a series of concentrations of MC-LR (0.1, 1.0, 10.0, 100.0 and 500.0μg/L) and Microcystis aeruginosa crude extracts (0.002 lysate, 0.02 lysate and 0.2 lysate) for 21days. Results showed that exposure to pure MC-LR can produce significant inhibitory effects on the photosynthetic characteristic parameters of rice, and the transpiration rate and stomatal conductivity are the most sensitive indicators in rice to MC-LR exposure. In contrast, different effect characteristics of photosynthesis parameters, even the stimulating and promoting effects, were found in rice under the exposure to Microcystis aeruginosa crude extracts (e.g. significant induction of the transpiration rate and stomatal conductivity of rice under the exposure to 0.02lysate). In addition, a series of ultrastructural alterations were also found in rice leaves exposed to high levels of the pure MC-LR and Microcystis aeruginosa crude extract. These results suggested that the toxicity mechanism of Microcystis aeruginosa crude extracts to rice is different from pure MC-LR and the decrease of biological toxicity might be due to the changes of MCs bio-availability caused by the interactions with other components co-existed in crude extract solution.
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Received: 03 May 2017
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[1] |
Wiegand C, Pflugmacher S. Ecotoxicological effects of selected cyanobacterial secondary metabolites a short review[J]. Toxicology and Applied Pharmacology, 2005,203(3):201-218.
|
[2] |
Spoof L, Neffling M R, Meriluoto J. Fast separation of microcystins and nodularins on narrow-bore reversed-phase columns coupled to a conventional HPLC system[J]. Toxicon, 2010,55(5):954-964.
|
[3] |
詹晓静,向垒,李彦文,等.农田土壤中微囊藻毒素污染特征及风险评价[J]. 中国环境科学, 2015,35(7):2129-2136.
|
[4] |
Lahti K, Rapala J, Frdig M, et al. Persistence of cyanobacterial hepatotoxin, microcystin-LR in particulate material and dissolved in lake water[J]. Water Research, 1997,3l(5):l005-10l2.
|
[5] |
Jones G J, Orr P T. Release and degradation of microcystin following algicide treatment of a Microcystis aeruginosa bloom in a recreational lake, as determined by HPLC and protein phosphatase inhibition assay[J]. Water Research, 1994,28(4):871-876.
|
[6] |
Craig M, Luu H A., McCready T L, et al. Molecular mechanisms underlying the interaction of motuporin and microcystins with type-1and type-2A protein phosphatases[J]. Biochemistry and cell biology, 1996,74(4):569-578.
|
[7] |
朱枫,钱晨,卢彦.微囊藻毒素诱导细胞氧化胁迫的研究进展[J]. 生态毒理学报[J]. 2010,5(6):769-775.
|
[8] |
Amado L L, Monserrat J M. Oxidative stress generation by microcystins in aquatic animals:why and how. Environ Int, 2010,6:226-235.
|
[9] |
Jiang J L, Gu X Y, Song R, et al. Microcystin-LR induced oxidative stress and ultrastructural alterations in mesophyll cells of submerged macrophyte Vallisneria natans (Lour.) Hara. Journal of Hazardous Materials, 2011,190(1-3):188-196.
|
[10] |
Ozawa K, Yokoyama A, Ishikawa K, et al. Accumulation and depuration of microcystin produced by the cyanobacterium Microcystis in freshwater snail[J]. Limnology, 2003,4:131-138.
|
[11] |
吴幸强,龚艳,王智,等.微囊藻毒素在滇池鱼体内的积累水平及分布特征[J]. 水生生物学报, 2010,34(2):388-393.
|
[12] |
Chen J, Zhang D W, Xie P, et al. Simultaneous determination of microcystin contaminations in various vertebrate (fish, turtle, duck and water bird) from a large eutrophic Chinese lake, Lake Taihu, with toxic Microcystis blooms[J]. Science of the Total Environment, 2009,407(10):3317-3322.
|
[13] |
Crush J R, Briggs L R, Sprosen J M, et al. Effect of irrigation with lake water containing microcystins on microcystin content and growth of ryegrass, clover, rape, and lettuce[J]. Environmental Toxicology, 2008,23(2):246-252.
|
[14] |
Chen W, Song L R, Gan N Q, et al. Sorption, degradation and mobility of microcystins in Chinese agriculture soils:risk assessment for groundwater protection[J]. Environmental Pollution, 2006,144(3):752-758.
|
[15] |
Corbel S, Mougin C, Bouaïcha N. Cyanobacterial toxins:modes of actions, fate in aquatic and soil ecosystems, phytotoxicity and bioaccumulation in agricultural crops[J]. Chemosphere, 2014, 96(2):1-15.
|
[16] |
Romero C S, Contardo V, Block T, et al. Accumulation of microcystin congeners in different aquatic plants and crops-A case study from lake Amatitlán, Guatemala[J]. Ecotoxicology and Environmental Safety 2014,102(1):121-128.
|
[17] |
Chen J, Han F X, Wang F, et al. Accumulation and phytotoxicity of microcystin-LR in rice(Oryza sativa)[J]. Ecotoxicology and Environmental Safety, 2012,76(2):193-199.
|
[18] |
张敏敏,姜锦林,周军英,等.环境相关浓度MC-LR作用对营养生长期水稻生长和抗氧化酶活性的影响[J]. 农业环境科学学报, 2014,33(12):2296-2302.
|
[19] |
Chen J, Song L, Dai J, et al. Effects of microcystins on the growth and the activity of superoxide dismutase and peroxidase of rape (Brassica napus L.) and rice (Oryza sativa L.)[J]. Toxicon, 2004, 43(4):393-400.
|
[20] |
耿志明,顾迎迎,王澎.微囊藻毒素对小白菜、番茄生长发育影响及其在它们体内积累的研究[J]. 江西农业学报, 2011,23(9):21-24.
|
[21] |
张慧,姜锦林,张宇峰,等.微囊藻毒素-LR和铜绿微囊藻裂解液对营养生长期水稻生理生化效应研究[J]. 中国环境科学, 2017,37(8):3134-3141.
|
[22] |
姜锦林,宋睿,任静华,等.蓝藻水华衍生的微囊藻毒素污染及其对水生生物的生态毒理学研究[J]. 化学进展, 2011,23(1):246-253.
|
[23] |
Jiang J L, Wang X R, Shan Z J, et al. Proteomic analysis of hepatic tissue of Cyprinus carpio L. exposed to cyanobacterial blooms in Lake[J]. Plos One, 2014,9(2):1-13.
|
[24] |
Peng L W, Ma J F, Chi W, et al. Low PSⅡ accumulation1 is involved in efficient assembly of photosystem Ⅱ in Arabidopsis thaliana[J]. Plant Cell, 2006,18(4):955-969.
|
[25] |
Chen X, Zhang W, Xie Y J, et al. Comparative proteomics of thylakoid membrane from a chlorophyll b-less rice mutant and its wild type[J]. Plant Science, 2007,173(4):397-407.
|
[26] |
Hu L F, Liang W Q, Yin C S, et al. Rice MADS3 regulates ROS homeostasis during late anther development[J]. Plant Cell, 2011, 23(2):515-533.
|
[27] |
Pflugmacher S. Possible allelopathic effects of cyanotoxins, with reference to microcystin-LR, in aquatic ecosystems[J]. Environmental Toxicology, 2002,17(4):407-413.
|
|
|
|