Anammox菌干粉菌剂制备与保藏技术

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Abstract The dry powder agent of anammox bacteria was prepared by vacuum drying method and stored at 4℃ for 30days which was protected from light and air. Meanwhile, the drying protective agent and correlated influence factors were screened in order to study the rejuvenative performance of the preserved anammox bacteria. Results showed that sodium alginate was the most suitable drying protective agent among these alternative protective agents of polyving akohol, carboxyl methyl cellulose, sodium alginate, trehalose and deionized water. Correspondingly, the optimized drying temperature and concentration of sodium alginate were 60℃ and 3% respectively. After rejuvenation of the preserved anammox bacteria for 30days, the activity of anammox bacteria had recovered to 41% compared to the fresh bacteria.

Key words： anammox drying protective agent sodium alginate optimal condition

Received: 01 April 2017

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	ZHANG Qian
	XU Xiao-chen
	WANG Chao
	WANG Xiao-jing
	YANG Feng-lin

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ZHANG Qian,XU Xiao-chen,WANG Chao等. Technique of preparation and preservation of dry powder agent of anammox bacteria[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(12): 4630-4636.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2017/V37/I12/4630

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