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| Analysis of microbial population of shortcut nitrification in ABR-MBR process |
| ZHAO Shi-hui1,2, LÜ Liang1,2, JIANG Zhi-yun1,2, WU Yi-ning1,2, WU Peng1,2,3, SHEN Yao-liang1,2,3 |
1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou Jiangsu 215009, China;
2. Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material, Suzhou Jiangsu 215009, China;
3. Key Laboratory of Environmental Science and Engineering of Jiangsu Province, Suzhou Jiangsu 215009, China |
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Abstract The effects of influent ammonium loading rate (ALR) on the species abundance of microbial communities and dominant bacterial in the ABR-MBR combined process were investigated by Miseq high-throughput sequencing. The results indicated that the numbers of ammonium oxidizing bacteria (AOB) can be significantly increased and the bio-activities of nitrite oxidizing bacteria (NOB) can be inhibited at the temperature of 28~32℃, pH of 7.1~7.4 and DO of 0.5~1mg/L by gradually increasing the influent ALR in the MBR. So that the shortcut nitrification will be achieved efficiently and stably. When influent ALR was 0.94kg/(m3·d), the average nitrite accumulation rate was above 60%, and NH4+-N removal rate was 90%. Proteobacteria were the dominant bacterial. The relative abundance of Nitrosomonas was increased from 4.97% to 22.56%, the relative abundance of Nitrospira was increased from 0.06% to 2.12% during the operation. Therefore, the nitrite accumulation rates were closely related to the bio-activities and abundance of AOB of shortcut nitrification the process. Its showed that shortcut nitrification can be efficiently achieved by a large number of AOB growth. Still, a slight increase of NOB abundance had little effect on the realization of shortcut nitrification. The microbial diversity and its functional structure stability were the guarantee of a stable and high removal efficiency in the ABR-MBR combined process.
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Received: 12 July 2017
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