小麦对纳米银的吸收及毒性响应

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (765 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract The uptake and toxicity of silver nanoparticles (AgNPs) to wheat were investigated.The wheat seedlings were exposed to different concentrations of AgNPs suspension in 4h.Oxidative stress and cell membrane damage were observed in roots.The content of malondialdehyde (MDA) and catalase (CAT) increased from (2.9±0.5) nmol/L/mgprot and (8.6±1.2) U/mgprot to (4.9±1.5) nmol/L/mgprot and (12.4±1.2) U/mgprot.The addition of L-cysteine reduced the toxicity of AgNO₃ to wheat and decreased the dissolved uptake rate constant of AgNO₃ from (275.4±12.3) L/(kg×h) to (210.8±11.2) L/(kg×h).In contrast,L-cysteine did not affect the toxicity of AgNPs to wheat,and the dissolved uptake rate constant did not differ significantly before and after the addition of L-cysteine [i.e.,(12.6±0.8) vs (11.2±0.6) L/(kg×h)].The results suggested that not only the released Ag⁺ from AgNPs,but also the particles themselves contributed to the bioavailability and toxicity of AgNPs.Furthermore,the dissolved uptake rate constants from different species of Ag in the presence of L-cysteine (i.e.,dissolved Ag⁺,AgNP particles,and Ag-cysteine) were calculated,and dissolved Ag⁺ exhibited the highest bioavailability [(275.4±12.3) L/(kg×h)],followed by Ag-cysteine complex [(210.8±11.2) L/(kg×h)]and finally the nanoparticles [1.6L/(kg×h)].A model described the uptake process of AgNPs were thus established.The predicted overall uptake rate constants were comparable to that observed in the experiment,which validated the success of the model in describing the uptake of AgNPs.

Key words： silver nanoparticles Ag⁺ wheat accumulation toxicity uptake rate constant

Received: 28 July 2017

X131

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WANG Qi
	LI Min
	WANG Rui
	SONG Ying-ling
	DANG Fei
	ZHOU Dong-mei

Cite this article:

WANG Qi,LI Min,WANG Rui等. The uptake and toxicity of silver nanoparticles to wheat[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(3): 1149-1156.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I3/1149

[1]	Chaloupka K, Malam Y, Seifalian A M. Nanosilver as a new generation of nanoproduct in biomedical applications[J]. Trends in Biotechnology, 2010,28(11):580-588.
[2]	Peng W, Menzies N W, Dennis P G, et al. Silver nanoparticles entering soils via the wastewater-sludge-soil pathway pose low risk to plants but elevated Cl concentrations increase Ag bioavailability[J]. Environmental Science & Technology, 2016,50(15):82-74.
[3]	Brunetti G, Donner E, Laera G, et al. Fate of zinc and silver engineered nanoparticles in sewerage networks[J]. Water Research, 2015,77(Supplement C):72-84.
[4]	周东美.纳米Ag粒子在我国主要类型土壤中的迁移转化过程与环境效应[J]. 环境化学, 2015,34(4):605-613.
[5]	吕继涛,张淑贞.人工纳米材料与植物的相互作用:植物毒性、吸收和传输[J]. 化学进展, 2013,1(1):156-163.
[6]	Barrena R, Casals E, Colón J, et al. Evaluation of the ecotoxicity of model nanoparticles[J]. Chemosphere, 2009,75(7):850-857.
[7]	Stampoulis D, Sinha S K, White J C. Assay-dependent phytotoxicity of nanoparticles to plants[J]. Environmental Science & Technology, 2009,43(24):9473.
[8]	林道辉,冀静,田小利,等.纳米材料的环境行为与生物毒性[J]. 科学通报, 2009,54(23):3590-3604.
[9]	Navarro E, Piccapietra F, Wagner B, et al. Toxicity of silver nanoparticles to Chlamydomonas reinhardtii[J]. Environmental Science & Technology, 2008,42(23):8959-8964.
[10]	Beer C, Foldbjerg R, Hayashi Y, et al. Toxicity of silver nanoparticles-nanoparticle or silver ion?[J]. Toxicology Letters, 2012,208(3):286-292.
[11]	Mili? M, Leitinger G, Pavi?i? I, et al. Cellular uptake and toxicity effects of silver nanoparticles in mammalian kidney cells:Cellular uptake and toxicity of nanosilver in mammalian kidney cells[J]. Journal of Applied Toxicology, 2015,35(6):581-592.
[12]	Wang P, Menzies N W, Lombi E, et al. Silver sulfide nanoparticles (Ag2S-NPs) are taken up by plants and are phytotoxic[J]. Nanotoxicology, 2015,9(8):1041-1049.
[13]	He D, Dorantes-Aranda J J, Waite T D. Silver nanoparticle-algae interactions:oxidative dissolution, reactive oxygen species generation and synergistic toxic effects[J]. Environmental Science & Technology, 2012,46(16):8731-8738.
[14]	何文寿,李生秀,李辉桃.营养液pH对小麦生长及吸收铵、硝态氮的影响[J]. 土壤, 1998,30(3):143-146.
[15]	Molleman B, Hiemstra T. Time, pH, and size dependency of silver nanoparticle dissolution:the road to equilibrium[J]. Environmental Science-Nano, 2017,4(6):1314-1327.
[16]	李敏,宋吟玲,党菲,等.水稻秸秆腐解液对水稻根部吸收纳米银(AgNP)速率的影响[J]. 生态与农村环境学报, 2016, 32(4):675-681.
[17]	Jiang C, Castellon B T, Matson C W, et al. Relative Contributions of Copper Oxide Nanoparticles and Dissolved Copper to Cu Uptake Kinetics of Gulf Killifish (Fundulus grandis) Embryos[J]. Environmental Science & Technology, 2017,51(3):1395-1404.
[18]	Li C C, Dang F, Li M, et al. Effects of exposure pathways on the accumulation and phytotoxicity of silver nanoparticles in soybean and rice[J]. Nanotoxicology, 2017,11(5):699-709.
[19]	Deng Y, Wang W, Yu P, et al. Comparison of taurine, GABA, Glu, and Asp as scavengers of malondialdehydein vitroandin vivo[J]. Nanoscale Research Letters, 2013,8(1):190.
[20]	Zhang J Q, Shen M, Zhu C C, et al. 3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse[J]. PloS One, 2014,9(2):e86589.
[21]	Luoma S N, Stoiber T, Croteau M N, et al. Effect of cysteine and humic acids on bioavailability of Ag from Ag nanoparticles to a freshwater snail[J]. NanoImpact, 2016,2(Supplement C):61-69.
[22]	Croteau M N, Luoma S N. A Biodynamic Understanding of Dietborne Metal Uptake by a Freshwater Invertebrate[J]. Environmental Science & Technology, 2008,42(5):1801-1806.
[23]	Li C C, Wang Y J, Dang F, et al. Mechanistic understanding of reduced AgNP phytotoxicity induced by extracellular polymeric substances[J]. Journal of Hazardous materials, 2016,308(Supplement C):21-28.
[24]	Yin L, Cheng Y, Espinasse B, et al. More than the ions:the effects of silver nanoparticles on Lolium multiflorum[J]. Environmental Science & Technology, 2011,45(6):2360-2367.
[25]	Larue C, Castillo-Michel H, Sobanska S, et al. Foliar exposure of the crop Lactuca sativa to silver nanoparticles:Evidence for internalization and changes in Ag speciation[J]. Journal of Hazardous materials, 2014,264(Supplement C):98-106.
[26]	Geisler-Lee J, Wang Q, Yao Y, et al. Phytotoxicity, accumulation and transport of silver nanoparticles by Arabidopsis thaliana[J]. Nanotoxicology, 2013,7(3):323-337.
[27]	Khan F R, Misra S K, Garcia-Alonso J, et al. Bioaccumulation dynamics and modeling in an estuarine invertebrate following aqueous exposure to nanosized and dissolved silver[J]. Environmental Science & Technology, 2012,46(14):7621-7628.
[28]	Zhou W, Liu Y L, Stallworth A M, et al. Effects of pH, Electrolyte, Humic Acid, and Light Exposure on the Long-Term Fate of Silver Nanoparticles[J]. Environmental Science & Technology, 2016,50(22):12214-12224.
[29]	Cvjetko P, Milosic A, Domijan A M, et al. Toxicity of silver ions and differently coated silver nanoparticles in Allium cepa roots[J]. Ecotoxicology and Environmental Safety, 2017,137(Supplement C):18-28.
[30]	Toh H S, Batchelor-Mcauley C, Tschulik K, et al. Chemical interactions between silver nanoparticles and thiols:a comparison of mercaptohexanol against cysteine[J]. 中国科学:化学, 2014,57(9):1199-1210.
[31]	Carpita N, Sabularse D, Montezinos D, et al. Determination of the pore size of cell walls of living plant cells[J]. Science, 1979, 205(4411):1144-1147.
[32]	Deng Y Q, White J C, Xing B S. Interactions between engineered nanomaterials and agricultural crops:implications for food safety[J]. Journal of Zhejiang University-Science A (Applied Physics & Engineering), 2014,15(8):552-572.
[33]	Miralles P, Church T L, Harris A T. Toxicity, Uptake, and Translocation of Engineered Nanomaterials in Vascular plants[J]. Environmental Science & Technology, 2012,46(17):9224-9239.
[34]	Wang S, Kurepa J, Smalle J A. Ultra-small TiO2 nanoparticles disrupt microtubular networks in Arabidopsis thaliana[J]. Plant Cell & Environment, 2011,34(5):811-820.