BaP和Cd单一复合对BaP蚯蚓亚细胞分配的影响

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Abstract

The influence of the calcium ion channel blocker (LaCl₃) or the SH-blocker (Nitrogen-ethylmaleimide, NEM) on the distribution and accumulation patterns of the Benzoapyrene (BaP) in the three subcellular fractions of earthworm Eisenia fetida (Fraction C:associated with the cytosol; Fractions D:associated with granules; and Fraction E:associated with tissue fragments and cell membranes) were explored under the condition of single BaP contamination and the combined contamination of the BaP and cadmium (Cd). The results showed that BaP was mainly accumulated in the Fraction E (about 55.42%~69.96% of the total content) the Fraction D (about 27.91%~32.90% of the total content) and Fraction C (about 2.13%~11.67% of the total content) no matter under single or combined pollution. Under the single pollution of BaP, the addition of LaCl₃and NEM could stimulate the BaP accumulation in three above subcellular fractions. While under the combined pollution, the effects were slightly different. LaCl₃ could stimulated the BaP accumulation in three above subcellular fractions, but NEM inhibited the BaP accumulation in the Fraction C (from 0.99mg/kg to 0.59mg/kg), and enhanced the BaP accumulation in the Fraction D and E. Therefore, calcium ion channel and the sulfhydryl protein took participate in the BaP distribution in earthworm, and Cd addition changed the BaP distribution compared with the single BaP contamination.

Key words： benzo[a]pyrene cadmium Eisenia fetida subcellular fraction blockers single pollution combined pollution

Received: 19 September 2017

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