水库与河流沉积物中好氧甲烷氧化菌群落差异性研究

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Abstract The aerobic methanotrophs play an important role in mitigating methane emissions and promoting the carbon-nitrogen cycle in the freshwater ecosystems. In this study, the influences of different water pollutant on aerobic methanotrophs were analyzed between the Miyun Reservoir and the North Canal at the Beijing Metropolitan. The results showed that differences of physiochemical between Miyun Reservoir and the North Canal were significantly, especially the main form of nitrogen. The main form of nitrogen in Miyun Reservoir was NO₃^--N, while NH₄⁺-N dominated in the North Canal. The physicochemical differences caused significantly influences on the MOB bacterial phylogenetic. Phylogenetic analyses revealed that the upstream freshwater highly contribute to the MOB sequences in Miyun Reservoir, and MOB has closely relationship with NO₃^--N. However, MOB sequences were mainly from activated sludge and wastewater in the North Canal, and MOB closely related with NH₄⁺-N. The different form of nitrogen in these two ecosystems showed significant influences not only on the MOB serious, but also the sources of MOB. Furthermore, the MOB OTUs showed higher modular microbial network in the North Canal than the Miyun Reservoir. The tightly connected species of MOB communities indicated that bacterial community composition was more vulnerable and sensitive to the various disturbances in the North Canal. The different forms of nitrogen influenced the MOB oxidation and MOB inhibitant activity in different extent, the influence of NH₄⁺-N on MOB was stronger than the influence of NO₃^--N on the Miyun Reservoir. The high concentration of NH₄⁺-N in the city river that would not only inhibitant the MOB bacteria oxidation but also promote the CH₄ release.

Key words： sediment aerobic methanotrophs nitrogen community structure phylogeny environmental factors co-occurrence network

Received: 26 October 2017

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	LIU Yang
	CHEN Yong-juan
	WANG Xiao-yan
	XU Kang-li
	Yang zhi-wei

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LIU Yang,CHEN Yong-juan,WANG Xiao-yan等. Microbial communities differences between aerobic methanotrophs in Miyun Reservoir and North Canal[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(5): 1844-1854.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I5/1844

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