有机磷阻燃剂甲状腺干扰效应及其作用机制研究——以磷酸三(2-氯丙基)酯(TCPP)为例

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Abstract In the present study, typical organophosphorus flame retardants (OPFRs), tris (2-chloropropyl) phosphate (TCPP), was selected as the object of study. GH3 cell proliferation assay was used to test the thyroid disrupting activity of TCPP; a two-hybrid thyroid receptor (TR) yeast assay, GH3 (TRβ-) cell proliferation assay and real time quantitative PCR were used to investigate the mechanism of action. The results revealed that TCPP decreased the GH3 cell proliferation at concentrations ranging from 1×10^-4mol/L to 2×10^-4mol/L. Furthermore, the results from two-hybrid TR yeast assay and GH3(TRβ-) cell proliferation assay suggested that TCPP could disrupt thyroid hormone activity through genomic and non-genomic pathway. The results of real time quantitative PCR supported the mRNA expression levels of c-fos, TRβ, integrin-av and k-ras were inhibited, suggesting that TCPP might induce thyroid hormone disruption by adjusting TRβ gene expression and activating α_vβ₃-ERK-1/2 signaling pathway. All of these results could provide the basic data and theoretical support for the environmental risk assessment of TCPP.

Key words： organophosphorus flame retardants (OPFRs) thyroid hormone disrupting effects mechanisms of action thyroid receptor

Received: 23 October 2017

171.5

Corresponding Authors: 李剑,副教授,lijian@bnu.edu.cn E-mail: lijian@bnu.edu.cn

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	SHEN Yang
	YU Chang
	KONG Dong-dong
	WANG Rong-fang
	LI Jian

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SHEN Yang,YU Chang,KONG Dong-dong等. Thyroid hormone disrupting effects of organophosphorus flame retardants and its mechanisms of action, take TCPP as an example[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(6): 2337-2344.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I6/2337

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