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| Reduction of Cr (VI) by Shewanella oneidensis MR-1 and its influencing factors |
| DU Yan-ying1, LIU Xiao-hong1,2, LI Jing1, LI Lei-ming1, SI You-bin1 |
1. Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China;
2. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China |
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Abstract The ability of Shewanella oneidensis MR-1 to reduce Cr (VI) was investigated by pure culture under anaerobic condition in laboratory. The characterics of Cr (VI) reduction by S. oneidensis MR-1 was studied by scanning electron microscopy (SEM) equipped with an energy dispersive spectrometer (EDS) and X-ray photoelectron spectroscopy (XPS). The results showed that the bioreduction of Cr (VI) at different initial concentrations was closely related to the tolerance of bacterial strains to chromium. Cr (VI) at low concentration had little effect on the strains growth. Cell growth was inhibited at high Cr (VI) concentration, thereby inhibiting the reduction of Cr (VI). The reduction of Cr (VI) increased with increasing the amount of strains inoculum. The optimum pH of the strains growth was neutral, and the weakly alkaline environment was more conducive to the Cr (VI) reduction than the acidic environment. The increasing amount of Fe (Ⅲ) accelerated the rate at which Cr (VI) was completely reduced. The results of SEM-EDS and XPS analysis showed that Cr (VI) and Cr (Ⅲ) existed on the surface of cells after treatment with Cr (VI) for 5days, which confirmed that Cr (VI) reduction by S. oneidensis MR-1was at the same time also accompanied by a small amount of Cr (VI) adsorption. Microbial reduction provides an effective method for the removal and detoxification of Cr (VI) in the environment.
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Received: 08 December 2017
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