The effect of organic matters and nitrite on the anaerobic ammonium oxidation (anammox)-denitrification coupling process was studied in a continuous flow reactor and batch tests. During the continuous flow experiment, the effect of glucose on the nitrogen removal performance of the anammox granules reactor was investigated with the addition of sufficient nitrite. As a result, the granules performed excellent anammox-denitrification coupling activities with the COD concentration of 100mg/L. While the COD concentration was improved to 200mg/L, the granules performed bad anammox-denitrification coupling activities. With the COD concentrations of 100 and 200mg/L, the corresponding ammonium removal activities of granules through anammox process were 0.096 and 0.071kg NH₄⁺-N/(kgVSS -d). The nitrite removal activities of granules through anammox process were 0.153 and 0.092kg NO₂^--N/(kgVSS -d). Meanwhile, the corresponding denitrification activities of the granules were 0.111 and 0.212kg NO₂^--N/(kgVSS -d). As for the batch tests, the effect of carbon sources and chemical oxygen demand (COD)/NO₂^--N ratios on the nitrogen removal performance of the anammox-denitrification coupling granules was analyzed. With the carbon source of glucose and the COD/NO₂^--N ranges of 1 to 4, the anammox bacteria performed greater nitrite competitive ability than denitrifiers. However, with the carbon source of sodium acetate and the COD/NO₂^--N ranges of 1 to 4, the anammox bacteria performed lower nitrite competitive ability than denitrifiers.