|
|
Influence of compost amendment on hydrocarbon degradation and microbial communities in petroleum contaminated soil |
WU Man-li, QI Yan-yun, ZHU Chang-cheng, CHEN Kai-li, XUE Peng-fei, XU Hui-ning |
Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, Xi'an University of Architecture and Technology, Xi'an 710055, China |
|
|
Abstract A laboratory study was conducted to investigate the effects of compost amendment on hydrocarbon degradation and microbial communities in petroleum contaminated soil. Petroleum hydrocarbons were analyzed using gravimetric method and GC-MS. Illumia Miseq technique was used to detect the microbial community structure of petroleum-polluted soil during the remediation. Results showed the removal efficiencies of total petroleum hydrocarbon (TPH), alkane, and polycyclic aromatic hydrocarbons (PAHs) were respectively (12.4±0.01)%, (10.2±0.01)%, and (9.38±0.02)% in the compost amendment soil (SC), compared to (3.21±0.02)%, (-3.00±0.01)%,and (-6.59±0.02)% in the control experiment (CK) after 42days of incubation. In response to compost amendment, the Shannon index, ACE index, and Chao1index increased from 4.30, 3489.3, 2691.0 to 5.80, 4684.7, and 3851.8, respectively. The relative abundance of Actinobacteria phyla decreased from 47.3% to 28.2%, and Bacteroidetes phyla increased from 0.78% to 16.2%. At the genus level, the relative abundance of the dominant genus in the petroleum contaminated soil including promicromonospora, Exiguobacterium, Nocardioides, Mycobacterium, and Citrobacter decreased significantly. Some new genus including Azomonas, Luteimonas, Pseudosphingo bacterium, and Parapedobacter appeared in the compost treated soil. The results indicated mature compost amendment effectively promoted petroleum hydrocarbon degradation in the soil, and soil microbial communities shifted significantly.
|
Received: 28 December 2017
|
|
|
|
|
[1] |
Wang X, Wang Q, Wang S, et al. Effect of biostimulation on community level physiological profiles of microorganisms in field-scale biopiles composed of aged oil sludge[J]. Bioresource Technology, 2012,111(3):308-315.
|
[2] |
刘五星,骆永明,滕应,等.石油污染土壤的生物修复研究进展[J]. 土壤, 2006,38(5):634-639.
|
[3] |
Gogoi B K, Dutta N N, Goswami P, et al. A case study of bioremediation of petroleum-hydrocarbon contaminated soil at a crude oil spill site[J]. Advances in Environmental Research, 2003,7(4):767-782.
|
[4] |
刘五星,骆永明.土壤石油污染与生物修复[M]. 北京:科学出版社, 2015:166-170.
|
[5] |
Shahi A, Aydin S, Ince B, et al. Evaluation of microbial population and functional genes during the bioremediation of petroleum-contaminated soil as an effective monitoring approach[J]. Ecotoxicology & Environmental Safety, 2016,125:153-160.
|
[6] |
Leys N M, Bastiaens L, Verstraete W, et al. Influence of the carbon/nitrogen/phosphorus ratio on polycyclic aromatic hydrocarbon degradation by Mycobacterium and Sphingomonas in soil[J]. Applied Microbiology & Biotechnology, 2005,66(6):726-736.
|
[7] |
Bento F M, Camargo F A, Okeke B C, et al. Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation[J]. Bioresource Technology, 2005,96(9):1049-55.
|
[8] |
叶茜琼,吴蔓莉,陈凯丽,等.微生物修复油污土壤过程中氮素的变化及菌群生态效应[J]. 环境科学, 2017,38(2):728-734.
|
[9] |
乔俊,陈威,张承东.添加不同营养助剂对石油污染土壤生物修复的影响[J]. 环境化学, 2010,29(1):6-11.
|
[10] |
杨茜,吴蔓莉,聂麦茜,等.石油污染土壤的生物修复技术及微生物生态效应[J]. 环境科学, 2015,36(5):1856-1863.
|
[11] |
马铁铮,马友华,付欢欢,等.生物有机肥和生物炭对Cd和Pb污染稻田土壤修复的研究[J]. 农业资源与环境学报, 2015,32(1):14-19.
|
[12] |
刘秀珍,马志宏,赵兴杰.不同有机肥对镉污染土壤镉形态及小麦抗性的影响[J]. 水土保持学报, 2014,28(3):243-247.
|
[13] |
孟桂元,周静,邬腊梅,等.改良剂对苎麻修复镉、铅污染土壤的影响[J]. 中国农学通报, 2012,28(2):273-277.
|
[14] |
Sayara T, Sarrà M, Sánchez A. Effects of compost stability and contaminant concentration on the bioremediation of PAHs-contaminated soil through composting[J]. Journal of Hazardous Materials, 2010,179(1):999-1006.
|
[15] |
郭婷,张承东,张清敏.生物修复石油污染盐碱土壤小试模拟系统中土壤性质与微生物特性变化[J]. 中国环境科学, 2010,30(8):1123-1129.
|
[16] |
Wu M L, Dick W A, Li W, et al. Bioaugmentation and biostimulation of hydrocarbon degradation and the microbial community in a petroleum-contaminated soil[J]. International Biodeterioration & Biodegradation, 2016,107(3):158-164.
|
[17] |
韩慧龙,陈镇,杨健民,等.真菌-细菌协同修复石油污染土壤的场地试验[J]. 环境科学, 2008,29(2):454-461.
|
[18] |
Wu M L, Li W, Dick W A, et al. Bioremediation of hydrocarbon degradation in a petroleum contaminated soil and microbial population and activity determination[J]. Chemosphere, 2017,169:124-130.
|
[19] |
Dibble J T, Bartha R. Effect of environmental parameters on the biodegradation of oil sludge[J]. Applied & Environmental Microbiology, 1979,37(4):729-39.
|
[20] |
Chaîneau C H, Rougeux G, Yéprémian C, et al. Effects of nutrient concentration on the biodegradation of crude oil and associated microbial populations in the soil[J]. Soil Biology & Biochemistry, 2005,37(8):1490-1497.
|
[21] |
Yanto D H Y, Tachibana S. Enhanced biodegradation of asphalt in the presence of Tween surfactants, Mn2+, and H2O2, by Pestalotiopsis sp. in liquid medium and soil[J]. Chemosphere, 2014,103(5):105-113.
|
[22] |
Lee S H, Lee S, Kim D Y, et al. Degradation characteristics of waste lubricants under different nutrient conditions[J]. Journal of Hazardous Materials, 2007,143(1):65-72.
|
[23] |
Wu G, Kechavarzi C, Li X, et al. Influence of mature compost amendment on total and bioavailable polycyclic aromatic hydrocarbons in contaminated soils[J]. Chemosphere, 2013,90(8):2240-2246.
|
[24] |
刘五星,骆永明,滕应,等.我国部分油田土壤及油泥的石油污染初步研究[J]. 土壤, 2007,39(2):247-251.
|
[25] |
Li H, Ye D, Wang X, et al. Soil bacterial communities of different natural forest types in Northeast China[J]. Plant & Soil, 2014,383(1/2):203-216.
|
[26] |
Sengupta A, Dick W A. Bacterial community diversity in soil under two tillage practices as determined by pyrosequencing[J]. Microbial Ecology, 2015,70(3):1-7.
|
[27] |
盛下放,何琳燕,胡凌飞.苯并[a]芘降解菌的分离筛选及其降解条件的研究[J]. 环境科学学报, 2005,25(6):791-795.
|
[28] |
周乐.多环芳烃降解菌的筛选、降解条件及其与玉米联合修复菲、芘污染土壤的研究[D]. 南京:南京农业大学, 2006.
|
[29] |
Zhao J K, Li X M, Zhang M J, et al. Parapedobacter pyrenivorans sp. nov. isolated from a pyrene-degrading microbial enrichment, and emended description of the genus Parapedobacter[J]. International Journal of Systematic & Evolutionary Microbiology, 2013,63(Pt 11):3994-3999.
|
[30] |
Pinyakong O, Habe H, Omori T. The unique aromatic catabolic genes in sphingomonads degrading polycyclic aromatic hydrocarbons (PAHs)[J]. Journal of General & Applied Microbiology, 2003,49(1):1-19.
|
[31] |
Zhou D P, Xia Y, Han R Y, et al. Isolation, identification and degradation characteristics of three phenanthrene-degrading bacteria[J]. Acta Scientiae Circumstantiae, 2003,23(1):124-128.
|
[32] |
袁军,赖其良,郑天凌,等.深海多环芳烃降解菌新鞘氨醇杆菌H25的降解特性及降解基因[J]. 微生物学报, 2008,48(9):1208-1213.
|
[33] |
游靖,李青,刘洋,等.一株高效驱油菌株HB-2降解原油活性的研究[J]. 生物技术通报, 2013,(11):170-174.
|
|
|
|