A comparative study on the kinetics and column experiment of As(Ⅲ) removal from groundwater by mature filter media formed in different ways was conducted. Besides,a variety of characterization methods were used to analyze the morphology and structure of the two film filter media. The results showed that the morphology was quite different. As(Ⅲ) could be removed more effectively by the chemical film filter media. The second-order model was able to simulate the kinetics of As(Ⅲ) removal well. The fitting rate constants of natural and chemical film filter media were 8.568×10^-6 and 14.248×10^-6 mg/(g·min), respectively. The Yan model has a fitting regression coefficient of R² >0.999 for the dynamic column breakthrough curve. At the same time, the experiment of desorption and regeneration was carried out. It was found that NaHCO₃ as a desorbent could effectively regenerate the filter media. According to the XRD results of the filter media before and after the filtration, the surface structure did not change much, thus the arsenic removal ability is stable. The content of iron and manganese oxides on the surface of film filter media directly affects the arsenic removal performance. Both film filter media consume hydroxyl groups during the arsenic removal process. The research provides theoretical support for the removal of arsenic from groundwater by different methods and has certain engineering guidance.