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| Effect of salinity on activity of nitrite-oxidizing bacteria and kinetic characterization |
| ZHANG Yu-kun1,2,3, ZHANG Liang1, DONG Yi-jun1, GU Sheng-bo1, PENG Yong-zhen1 |
1. National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China;
2. School of Civil Engineering, Dalian Nationalities University, Dalian 116600, China;
3. Jiangsu Tianyu Environmental protection Group CO. Ltd., Yangzhou 225200, China |
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Abstract In order to analyze the effect of salinity on NOB (nitrite-oxidizing bacteria) activity and kinetic characterization, NOB was enriched in a lab-scale SBR system with high concentration of nitrite wastewater in this study. The FISH (fluorescence in situ hybridization) analysis showed that Nitrobacter accounted for(81%±6%)of total bacteria. The maximum specific nitrite oxidation rate was (42.5±0.9)mgN/(gVSS·h). The effect of salinity on NOB activity was investigated using this mixed culture. The kinetic parameters of NOB (Ko, KS) were both measured under salinity of 10g/L. Results showed that compared with salinity 0g/L, the activity of NOB decreased 3.3% unders alinity of 15g/L and decreased 11% under salinity of 10, 20g/L. When salinity was 10g/L, the maximum specific nitrite oxidation rate was (37.9±0.7)mgN/(gVSS·h). The half-saturation constant for oxygen (Ko) and nitrite (KS) were (1.51±0.06), (6.06±0.15) mg/L. The values of Ko and KS were higher than recommended value of ammonia-oxidizing bacteria (AOB) in ASM2model. It is suggested that salinity could reduce maximum specific nitrite oxidation rate and affect the oxygen affinity and substrate (nitrite) affinity of NOB.
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Received: 27 September 2019
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