ZnO-NPs对好氧反硝化细菌<i>Zobellella</i> sp. B307的致毒机制

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Abstract In this paper, a strain of aerobic denitrifying bacteria Zobellellasp. B307screened from the sediment of Jiaozhou Bay was studied. Under short-term exposure condition, the toxic effect of ZnO-NPs on the bacterial strain was analysed through the changes in growth, denitrification ability, related enzyme activity and metabolic pathway of the bacteria. The toxicity mechanism of ZnO-NPs on the strain was discussed based on the dissolution test of zinc ions and the oxidative stress levels such as of CAT and ROS. The results showed that comparing with the control group, the addition of ZnO-NPs at 200mg/L could decrease the nitrate removal rate and NIR activity of the strain to 57.53% and 14.46%. Whereas it could increase the LDH、ROS level、SOD activity to 378%、534% and 60.32% respectively. ZnO-NPs could induce the formation of reactive oxygens species and through that, the membrane permeability of strain was changed, the enzyme activity was decreased, and the related pathway of protein, amino acid synthesis and gene expression were affected, which inhibited the denitrification ability of the strain. The production of free zinc ions is not the main cause of toxicity of ZnO-NPs on the bacterial strain.

Key words： Nano zinc oxide (ZnO-NPs) aerobic denitrifying bacteria metabolites oxidative stress

Received: 10 January 2020

PACS:

X703.5

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	Articles by authors
	WU Chun-han
	BAI Jie
	ZHAO Yang-guo
	REN Zhao-meng
	CHEN Lin
	LI Kui-ran

Cite this article:

WU Chun-han,BAI Jie,ZHAO Yang-guo等. Toxicity and mechanism of ZnO-NPs on an aerobic denitrifying bacteria strain Zobellella sp.B307[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(8): 3644-3653.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I8/3644

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