黄曲霉A5p1对偶氮染料的降解特性和降解途径

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Abstract Aspergillus flavus strain A5p1 was used as a biological material to study its broad decolorization spectrum and decolorization characteristics and degradation products with the azo dye Direct Blue 71 (DB71) as the model substrate. The results showed that the decolorization efficiency of 15 kinds of dyes ranged from 61.7% to 100.0% with the 100mg/L dye concentration. There might be a synergistic effect of biosorption and biodegradation on the decolorization. The optimum decolorization condition of A5p1 for azo dye DB71 was pH 7.0, temperature 30℃, dye concentration 300mg/L, and sucrose as a carbon source. Enzyme analysis revealed that glucose oxidase and manganese peroxidases were involved in the degradation process. FTIR, GC-MS and LC-MS analyses indicated that the intermediates of azo dye degradation included naphthalene amine, naphthalene diazonium, 2-hydroxy-6-oxal-yl-benzoic acid and 1-naphthol.

Key words： fungi dye degradation enzyme

Received: 14 January 2020

PACS:

X703

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	CHENG Ning
	LI Qing-yun
	LIU You-yan

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CHENG Ning,LI Qing-yun,LIU You-yan. Decolorization exploration of azo dye by Aspergillus flavus A5p1: characteristics and biodegradation pathway[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(8): 3367-3374.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I8/3367

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