硫自养反硝化系统运行效能和微生物群落结构研究

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Abstract The sulfur-based autotrophic denitrification reactor was set up and its denitrification performance was evaluated in this study. The denitrification activity of the sludge was determined by batch tests, and the microbial community structure was revealed by scanning electron microscopy(SEM) and high-throughput sequencing. The influent NO₃^--N concentration of the reactor operated with sequencing batch mode was 80mg/L. The autotrophic denitrification performance increased with the hydraulic retention time (HRT) gradually shortened from 12h to 6h, the total inorganic nitrogen(TIN) removal efficiency and the average TIN removal loading rate of the reactor during the stable period were 99.1% and 0.158kg N/(m³·d), respectively. The maximum NO₂^--N concentration during the SBR cycle was 13.3mg/L, the pH value decreased from 7.38 to 6.94 during the reduction process from NO₃^--N to NO₂^--N, and the pH value remained stable during the process of reducing NO₂^--N to N₂. The batch test results showed that the NO₃^--N removal rate of sulfur autotrophic denitrifying bacteria and heterotrophic denitrifying bacteria were 0.515and 0.196kg N/(kg VSS·d), respectively, and the NO₂^--N removal rate of sulfur autotrophic denitrifying bacteria was 0.117kg N/(kg VSS·d), the sludge had both autotrophic denitrification activity and heterotrophic denitrification activity. SEM images showed that both rod-shaped bacteria and spherical bacteria were observed in the sludge. The dominant sulfur autotrophic denitrifying bacteria in the sludge were Thiobacillus, Sulfurimonas and Thermomonas, with relative abundance of 14.5%, 7.6% and 6.0%, respectively.

Key words： sulfur-based autotrophic denitrification sulfur electron donor sequencing batch reactor nitrogen removal activities high throughput sequencing

Received: 06 February 2020

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X703.1

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	Articles by authors
	MA Xiao-ran
	ZHENG Zhao-ming
	BIAN Wei
	LI Jun
	ZHOU Rong-xuan
	YANG Jing-yue

Cite this article:

MA Xiao-ran,ZHENG Zhao-ming,BIAN Wei等. Study on operation efficiency and microbial community structure of sulfur-based autotrophic denitrification system[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(10): 4335-4341.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I10/4335

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