基于氨氮,硝氮及乙酸条件下Anammox菌的培养

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Abstract In order to investigate the characteristic of anammox enrichment process with ammonium, nitrate and acetic acid as substrates, an MBBR with mediums from A²/O system treating municipal wastewater was established to start up anammox process. The results showed that the enrichment of anammox bacteria, with ammonium, nitrate and acetic acid as substrates, combined with denitrification process could be obtained rapidly. TN removal load reached to 920.79mg/(m²·d), and Anammox activity could attain to 3018.19mg/(m²·d) after 130d enrichment. Quantitative PCR results showed that after enrichment, the proportion of Ca. Brocadia increased from 0.89% to 27.80%, which is the dominated community of anammox bacteria. The proportion of Thauera increased from 0.01% to 6.75%, and the proportion of Flavobacterium increased from 0.29% to 11.72%, which are the dominated communities of partial denitrifying bacteria.

Key words： anaerobic ammonium oxidation enrichment moving-bed biofilm reactor biological nitrogen removal

Received: 10 March 2020

PACS:

X703.5

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I10/4348

[1]	Kartal B, Kuenen J G, Loosdrecht M C M V. Sewage Treatment with Anammox[J]. Science, 2010,328(5979):702-703.
[2]	Zhang L, Narita Y, Gao L, et al. Microbial competition among anammox bacteria in nitrite-limited bioreactors[J]. Water Research, 2017,125:249-258.
[3]	Azari M, Walter U, Rekers V, et al. More than a decade of experience of landfill leachate treatment with a full-scale anammox plant combining activated sludge and activated carbon biofilm[J]. Chemosphere, 2017,174:117-126.
[4]	Zhang L, Narita Y, Gao L, et al. Maximum specific growth rate of anammox bacteria revisited[J]. Water Research, 2017,116:296-303.
[5]	Wang G, Xu X, Zhou L, et al. A pilot-scale study on the start-up of partial nitrification-anammox process for anaerobic sludge digester liquor treatment[J]. Bioresource Technology, 2017,241:181-189.
[6]	Lopez H, Puig S, Ganigue R, et al. Start-up and enrichment of a granular anammox SBR to treat high nitrogen load wastewaters[J]. Journal of Chemical Technology & Biotechnology, 2008,83(3):233-241.
[7]	Jetten M S M, Strous M, Passchoonen K V D, et al. The anaerobic oxidation of ammonium[J]. FEMS Microbiology Reviews, 1998, 22(5):421-437.
[8]	Zhu W, Li J, Dong H, et al. Effect of influent substrate ratio on anammox granular sludge:performance and kinetics[J]. Biodegradation, 2017,28:437-452.
[9]	Xi T, Guo Y, Jiang B, et al. Metagenomic approaches to understanding bacterial communication during the anammox reactor start-up[J]. Water Research, 2018,136:95-103.
[10]	Li J, Peng Y, Zhang L, et al. Quantify the contribution of anammox for enhanced nitrogen removal through metagenomic analysis and mass balance in an anoxic moving bed biofilm reactor[J]. Water Research, 2019,160:178-187.
[11]	Wett B, Omari A, Podmirseg S M, et al. Going for mainstream deammonification from bench to full scale for maximized resource efficiency[J]. Water Science & Technology, 2013,68(2):283-289.
[12]	Kamp A, Ottosen L D M, Thøgersen N B, et al. Anammox and partial nitritation in the mainstream of a wastewater treatment plant in a temperate region (Denmark)[J]. Water Science & Technology, 2019, 79:1397-1405.
[13]	Yeshi C, Hong K B, Loosdrecht M C M V, et al. Mainstream partial nitritation and anammox in a 200,000m3/day activated sludge process in Singapore:scale-down by using laboratory fed-batch reactor[J]. Water Science & Technology, 2016,74(1):48-56.
[14]	Gu J, Yang Q, Liu Y. Mainstream anammox in a novel A-2B process for energy-efficient municipal wastewater treatment with minimized sludge production[J]. Water Research, 2018,138:1-6.
[15]	CastroBarros C M, Jia M, Loosdrecht M C M V, et al. Evaluating the potential for dissimilatory nitrate reduction by anammox bacteria for municipal wastewater treatment[J]. Bioresource Technology, 2017, 233:363-372.
[16]	Chen K, Zhang L, Sun S, et al. In situ enrichment of anammox bacteria in anoxic biofilms are possible due to the stable and long-term accumulation of nitrite during denitrification[J]. Bioresource Technology, 2020,300:1-7.
[17]	Graaf A A V D, De B P, Robertson L A, et al. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor[J]. Microbiology, 1996,142(8):2187-2196.
[18]	Amann R, Krumholz L R, Stahl D A. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology[J]. Journal of Bacteriology, 1990,172(2):762-770.
[19]	Amann R, Binder B, Olson R, et al. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations[J]. Applied and Environmental Microbiology, 1990,56(6):1919-1925.
[20]	Daims H, Bruhl A, Amann R, et al. The Domain-specific Probe EUB338is Insufficient for the Detection of all Bacteria:Development and Evaluation of a more Comprehensive Probe Set[J]. Systematic and Applied Microbiology, 1999,22(3):434-444.
[21]	Kartal B, Rattray J E, Van N L, et al. Candidatus "Anammoxoglobus propionicus" a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria[J]. Systematic and Applied Microbiology, 2007,30(1):39-49.
[22]	Strous M, Kuenen J G, Jetten M S. Key physiology of anaerobic ammonium oxidation[J]. Applied and Environmental Microbiology, 1999,65(7):3248-3250.
[23]	Tang C, Zheng P, Mahmood Q, et al. Start-up and inhibition analysis of the Anammox process seeded with anaerobic granular sludge[J]. Journal of Industrial Microbiology & Biotechnology, 2009,36(8):1093-1100.
[24]	董晓莹,彭党聪.不同碳氮比下污水反硝化过程中亚硝氮积累的特性研究[J]. 环境科学学报, 2017,37(9):3349-3355. Dong X Y, Peng D C. Nitrite accumulation in denitrification with different C/N ratios[J]. Acta Scientiae Circumstantiae, 2017,37(9):3349-3355.
[25]	陈国燕,彭党聪,李惠娟,等.厌氧氨氧化耦合部分反硝化处理低浓度氨氮废水[J]. 环境工程学报, 2018,12(7):1888-1895. Chen G Y, Peng D C, Li H J, et al. Treating low-nitrogenous wastewater by coupling anammox with partial denitrification[J]. Chinese Journal of Environmental Engineering, 2018,12(7):1888-1895.
[26]	王维奇,王秀杰,李军,等.部分反硝化耦合厌氧氨氧化脱氮性能研究[J]. 中国环境科学, 2019,39(2):641-647. Wang W Q, Wang X J, Li J, et al. Study on the performance of partial denitrification coupled with anaerobic ammonia oxidation for nitrogen removal[J]. China Environmental Science, 2019,39(2):641-647.
[27]	Kalyuzhnyi S, Marina G. DEAMOX-New microbiological process of nitrogen removal from strong nitrogenous wastewater[J]. Desalination, 2009,248:783-793.
[28]	Strous M, Heijnen J J, Kuenen J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology and Biotechnology, 1998,50(5):589-596.
[29]	ClaracReino, Suárez-Ojeda M E, Pérez J, et al. Stable long-term operation of an upflow anammox sludge bed reactor at mainstream conditions[J]. Water Research, 2018,128:331-340.
[30]	Laureni M, Falas P, Robin O, et al. Mainstream partial nitritation and anammox:Long-term process stability and effluent quality at low temperatures[J]. Water Research, 2016,101:628-639.
[31]	Srinandan C S, Mrinal S, Bhavita P, et al. Assessment of denitrifying bacterial composition in activated sludge[J]. Bioresource Technology, 2011,102(20):9481-9489.
[32]	Payne W J. Reduction of nitrogenous oxides by microorganisms[J]. Bacteriological Reviews, 1974,37(4):409-452.
[33]	Qian W, Ma B, Li X, et al. Long-term effect of pH on denitrification:High pH benefits achieving partial denitrification[J]. Bioresource Technology, 2019,278:444-449.