温带亚高山针叶林土壤碳循环微生物群落的时空动态

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Abstract The cbbM, amylase and cellulase genes were selected as molecular markers to analyze functional microbial groups using real-time quantitative PCR method. We investigated the spatiotemporal dynamics of abundance of functional microbial groups in soils of four forests:Larix gmelinii var. principis rupprechtii, Picea meyeri, P. Wilsonii and Pinus tabulaeformis in subalpine temperate of north China. The results indicated that soil content of total carbon (TC), total nitrogen (TN), total sulfur (TS), organic matter (OM) and total organic carbon (TOC), pH, ammonium nitrogen (NH₄⁺-N), nitrate nitrogen(NO₃^--N), and the activities of catalase, sucrase and urease were different in the soil of four forests, and exhibited seasonal dynamics variation. The contents of TC, TN, TS, C/N, OM and TOC were the highest, but pH value was the lowest in the higher altitude L. principis forest soil. The contents of TC, TN, nitrite nitrogen (NO₂^--N), sucrase activity and urease activity were significantly correlated with the abundance of microbial groups involving in carbon fixation, starch degradation and cellulose degradation. The content of NO₃^--N in soil was significantly related to the relative abundance of carbon decomposing and carbon fixing microorganisms. Soil C/N, NO₂^--N, OM, TOC content, pH, catalase and urease activity were significantly correlated with the relative abundance of microbial groups involving in degradation of labile carbon and recalcitrant carbon. Vegetation type and seasonal change jointly affected the abundance of soil functional microbial groups, but seasonal change was the dominant factor. The vegetation and soil environmental factors affected the carbon source-sink balance of forest soil by regulating the structure of carbon metabolism functional groups of microbial community.

Key words： subalpine coniferous forest microbial community soil carbon cycle functional microbial group real time quantitative PCR

Received: 08 March 2020

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	LI Cui
	QIAO Sha-sha
	LIU Jian-xian
	CHAI Bao-feng

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LI Cui,QIAO Sha-sha,LIU Jian-xian等. Spatiotemporal dynamics of microbial communities involved in soil carbon cycling of subalpine temperate coniferous forest[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(10): 4540-4548.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I10/4540

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