二氯乙腈对大肠杆菌基因表达的影响

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Abstract To explore the effects of dichloroacetonitrile (DCAN) on gene expression of E.coli and corresponding toxic effect, the gene expression of E.coli under different dosages of DCAN for 120min was identified by self-organizing map (SOM) clustering and dose-response relationship. The results showed that the genes expression was dynamically affected under varied exposure time and concentration of DCAN. When the concentration of DCAN was 1.429×10^-3mg/L, the activity of multiple gene promoter included in SOS response regulation, oxidative and general stress had been changed. These consequences further resulted in DNA damage and more severe oxidative stress on the cells, made them bio-chemically and physically unstable. The results also clarified that DNA damage is the main toxic mode of DCAN through different pathways.

Key words： dichloroacetonitrile gene expression toxic effect DNA damage

Received: 21 August 2020

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X172

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	LI Dong
	CHENG Wen
	QIN Lu
	REN Jie-hui
	ZHENG Xing

Cite this article:

LI Dong,CHENG Wen,QIN Lu等. Effects of dichloroacetonitrile on gene expression of Escherichia coli[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(3): 1398-1404.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I3/1398

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