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| Effects and mechanisms of modified-biochar on biodegradation of antibiotics as revealed by bacteriological characteristics |
| YANG Fang, JIAN Hong-xian, GAO Yue, WANG Cui-ping |
| Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China |
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Abstract Three kinds of modified-biochar including MBC350, MBC500 and MBC700, and two types of antibiotics sulfamethoxazole (SMX) and chloramphenicol (CAP), were selected to investigate effects of different MBCs on biodegradation of SMX and CAP and the bacteriological characteristics of Pseudomonas stutzeri and Shewanella putrefaciens. The results showed that in the bacterial system cultured with low-concentration MBCs, the removal of SMX and CAP mainly depended on biodegradation by P. Stutzeri and S. putrefaciens. While in the bacterial system cultured with high-concentration MBCs, the removal of SMX and CAP was mainly by the adsorption onto MBCs. The adsorption capacities of SMX and CAP onto MBCs and the bacterial growth all increased as the concentrations of MBCs increased, leading to the decrease in the concentrations of antibiotics in the solution and the biodegradation efficiency. The content of fatty acids of P. stutzeri was significantly improved, while the fatty acids synthesis of S. putrefaciens was inhabited in the presence of MBCs. In particular, the components C10:0, C15:1, cis-10 of P. stutzeri were disappeared, and the components of trans-unsaturated fatty acids of S. putrefaciens were generated, such as C14:1, cis-9 and C15:1, cis-10. Additionally, by using absolute quantitative technique, the gene copies number of P. stutzeri was clearly improved, while it was inhabited for S. putrefaciens in the presence of MBCs. But the gene copies numbers of P. stutzeri and S. putrefaciens were increased with the increasing concentrations of MBCs. Therefore, this study showed that low-concentration MBCs were conducive to the biodegradation of SMX and CAP. While the bacteriological characteristics, such as bacterial growth, fatty acid and copy number of bacterial gene expression, were all promoted in the presence of high-concentration MBCs.
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Received: 05 September 2020
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