弥河沉积物的反硝化和厌氧氨氧化过程

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Abstract Four sites were selected along Mihe River to sample the sediments and characterize physiochemical property to reveal denitrification/anammox processes and their constraints by isotope tracing experiment and molecular biological analysis. The results showed that the rates of denitrification varied from 151.75 to 2847.86μmol/(m²·h), and the anammox rates were in the range of 149.57 to 2109.17μmol/(m²·h). The relative contribution of anammox in the nitrogen removal was in an average of 56.1%. The nirK type was the dominant denitrifier with the abundance of 0.19×10⁶~5.12×10⁶ copies/g in the sediment, which was in the phylum of α- and β- Proteobacteria. The anammox bacteria marked by hzsA gene was in the range of 2.58×10²~1.14×10⁴ copies/g, which belonged to the Ca. Brocadia clades in Planctomycetes. Furthermore, the denitrification rate was positively related with TN content and PO₄^3- concentration, while the anammox rate was positively related with TN content and negatively related with sediment density. The physiochemical property of sediment was the main condition determining the nitrogen removal rates. The results highlight that denitrification and anammox were of great significance to reduce nitrogen eutrophication, and sediment environment treatment was a considerable way to regular nitrogen removal rate.

Key words： denitrification anammox functional bacteria impact factor Mihe River

Received: 10 August 2020

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X524

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	LI Jia-lin
	QIN Song

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LI Jia-lin,QIN Song. Denitrification and anammox processes in sediment of Mihe River, China[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(4): 1588-1596.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I4/1588

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