高盐度复杂染料废水脱色菌群的降解特性

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Abstract In this study, a bacterial consortium was isolated from municipal activated sludge from Tianjin, China. Identification via 16SrDNA sequencing showed that it was mainly consisted of Dokdonella (12.26%), Dyella (10.19%), Sacharibacteria genera (7.07%), Rhodobacter (5.80%), Pseudodoxanthomonas (3.61%), and Flavihumibacter (3.02%). The decolorization conditions of high-salinity complex dyes wastewater, such as co-metabolic substrates as well as various physical and chemical parameters, were studied. The results showed that the best co-metabolic substations for the decolorization of complex dyes wastewater were glucose and ammonium sulfate. The optimum decolorization conditions were 35℃, pH 9.0, and inoculum concentration of 1.2g/L. Under the condition of high salinity (50g/L), the decolorization efficiency of complex dyes wastewater reached (87±2.4) % within 72h. Methyl orange was degraded first, followed by disperse blue, while acid fuchsin had a slow degradation rate. The chromophore groups (-N=N-, -NH₂, and -SO₃) and the benzene rings of complex dyes were destroyed, as identified with by high performance liquid chromatography and Fourier transform infrared spectroscopy. Degradation metabolites included aniline, acetamide, and 2-amino-5-methylbenzoic acid as identified by gas chromatography mass spectrometry.

Key words： complex dyes high salinity bacterial consortium biodegradation

Received: 13 October 2020

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X511

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	LI Jin-jia
	FAN Xiao-dan
	ZHANG Dao-hong
	YUAN Zheng-tong
	ZHOU Jia-ying
	WANG Xue-qi

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LI Jin-jia,FAN Xiao-dan,ZHANG Dao-hong等. Biodegradation characteristics of high-salinity complex dyes wastewater with high salinity by the bacterial consortium[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(6): 2631-2638.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I6/2631

[1]	Seyedi Z S, Kashi F J, Zahraei Z. Isolation, characterization and decolorization of disperse blue 60 by newly isolated bacterial strains from Kashan textile wastewater[J]. Water Environment Research, 2020,92(4):873-879.
[2]	Afiya H, Ahmet E E, Shah M M. Enzymatic decolorization of remazol brilliant blue royal (RB 19) textile dye by white rot fungi[J]. Journal of Advanced Applied Scientific Research, 2019,4(1):11-15.
[3]	张饮江,相元泉,鲁仙,等.零价铁激活过硫酸盐降解水中铬黑T特性[J]. 中国环境科学, 2020,40(2):653-660. Zhang Y J, Xiang Y Q, Lu X, et al. Degradation of chromium black T in water by persulfate activated with zero-valent iron[J]. China Environmental Sciences, 2020,40(2):653-660.
[4]	王怡琴,谢学辉,郑秀林,等.激活剂促进微生物降解偶氮、蒽醌和三苯甲烷类染料研究进展[J]. 化工进展, 2019,38(6):2968-2976. Wang Y Q, Xie X H, Zheng X L, et al. Advances in research on activators promoting microbial degradation of dyes[J]. Chemical Industry and Engineering Progress, 2019,38(6):2968-2976.
[5]	Shahi A, Rai B N, Singh R S. Biodegradation of reactive orange 16 dye in microbial fuel cell:an innovative way to minimize waste along with electricity production[J]. Applied Biochemistry and Biotechnology, 2020,192(2):196-210.
[6]	Franca R D G, Pinheiro H M, Loureno N D. Recent developments in textile wastewater biotreatment:dye metabolite fate, aerobic granular sludge systems and engineered nanoparticles[J]. Reviews in Environmental Science and Bio/Technology, 2020,19(1):149-190.
[7]	Rosu C M, Avadanei M, Gherghel D, et al. Biodegradation and detoxification efficiency of azo-dye reactive orange 16by Pichia kudriavzevii CR-Y103[J]. Water, Air, and Soil Pollution, 2018,229(1):1-18.
[8]	郭建博,周集体,王栋,等.耐盐菌群对高含盐染料模拟废水的脱色实验研究[J]. 环境污染治理技术与设备, 2005,6(12):31-36. Guo J B, Zhou J T, Wang D, et al. Decolorization of azo dyes with high salt concentration by salt-tolerant mixed cultures under anaerobic conditions[J]. Techniques and Equipment for Environmental Pollution Control, 2005,6(12):31-36.
[9]	Vyrides I, Bonakdarpour B, Stuckey D C. Salinity effects on biodegra-detion of reactive black 5 for one stage and two stages sequential anaerobic aerobic biological processes employing different anaerobic sludge[J]. International Biodeterioration and Biodegradation, 2014,95:294-300.
[10]	Amaral F M, Kato M T, Florêncio L, et al. Color, organic matter and sulfate removal from textile effluents by anaerobic and aerobic processes[J]. Bioresource Technology, 2014,163:364-369.
[11]	Li M, Yao Y J, Zhang W, et al. Fractionation and concentration of high-salinity textile wastewater using an ultra-permeable sulfonated thin-film composite[J]. Environmental Science and Technology, 2017,51(16):9252-9260.
[12]	Ogugbue C J, Sawidis T, Oranusi N A. Evaluation of colour removal in synthetic saline wastewater containing azo dyes using an immobilized halotolerant cell system[J]. Ecological Engineering, 2011,37(12):2056-2060.
[13]	Yaseen D A, Scholz M. Comparison of experimental ponds for the treatment of dye wastewater under controlled and semi-natural conditions[J]. Environmental Science and Pollution Research, 2017, 24(19):16031-16040.
[14]	翟俊,柳沛松,赵聚姣.过一硫酸盐碱催化处理染料废水[J]. 中国环境科学, 2020,40(2):647-652. Zhai J, Liu P S, Zhao J J. Catalytic treatment of dyestuff wastewater by persulphate salt and alkali[J]. China Environmental Sciences, 2020,40(2):647-652.
[15]	郝思宇,张艾,刘亚男.臭氧与过氧化钙协同降解甲基红废水[J]. 中国环境科学, 2019,39(2):591-597. Hao S Y, Zhang A, Liu Y N. Removal of methyl red in aqueous by O3/CaO2 treatment:influencing factors and synergetic effects[J]. China Environmental Sciences, 2019,39(2):591-597.
[16]	Asad S, Amoozegar M A, Pourbabaee A A, et al. Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria[J]. Bioresource Technology, 2007,98(11):2082-2088.
[17]	李彬,王志伟,安莹,等.盐度对膜-生物反应器污泥表观硝化速率的抑制机理[J]. 中国环境科学, 2014,34(2):371-377. Li B, Wang Z W, An Y, et al. Inhibition mechanisms of apparent nitrification rate in membrane bio-reactor with salinity[J]. China Environmental Science, 2014,34
[18]	杨阳,胡翔.酸性红B高效降解耐盐菌株BY-2的分离鉴定及降解特性研究[J]. 高校化学工程学报, 2016,30(3):734-740. Yang Y, Hu X. Isolation and identification of a salt-tolerant bacteria strain BY-2 for acid red B degradation[J]. Journal of Chemical Engineering of Chinese Universities, 2016,30(3):734-740.
[19]	郑红叶,薛雅蓉,刘常宏.裂褶菌cfcc7252菌株对孔雀石绿染料的高效降解[J]. 微生物学通报, 2017,44(1):38-48. Zheng H Y, Xue Y R, Liu C H. Degradation of malachite green dye by Schizophyllum commune cfcc7252[J]. Microbiology China, 2017, 44(1):38-48.
[20]	Zhao M, Sun P F, Du L N, et al. Biodegradation of methyl red by Bacillus sp. strain UN2:decolorization capacity, metabolites characterization, and enzyme analysis[J]. Environmental Science and Pollution Research International, 2014,21(9):6136-6145.
[21]	Cheng N, Li Q Y, Tang A X, et al. Decolorization of a variety of dyes by Aspergillus flavus A5p1[J]. Bioprocess and Biosystems Engineering, 2018,41(4):511-518.
[22]	Parmar N D, Shukla S R. Biodegradation of anthraquinone based dye using an isolated strain Staphylococcus hominis subsp. hominis DSM 20328[J]. Environmental Progress and Sustainable Energy, 2017,37(1):203-214.
[23]	Palma T L, Donaldben M N, Costa M C, et al. Putative Role of Flavobacterium, Dokdonella and Methylophilus strains in paracetamol biodegradation[J]. Water, Air, and Soil Pollution, 2018, 229(6):1-23.
[24]	Zhao L, Zhou R, Jia R, et al. Biodecolorization of acid red GR by a newly isolated Dyella ginsengisoli LA-4using response surface methodology[J]. Journal of Hazardous Materials, 2010,181(1):602-608.
[25]	赵凯,于影,姜丹,等.1株类球红细菌及其降解敌敌畏的特性[J]. 环境科学, 2009,30(4):1199-1204. Zhao k, Yu Y, Jiang D, et al. Degradation of dichlorvos by Rhodobacter sphaeroides[J]. Environmental Science, 2009,30(4):1199-1204.
[26]	Liu Y, Jin J H, Liu H C, et al. Dokdonella immobilis sp. nov. isolated from a batch reactor for the treatment of triphenylmethane dye effluent[J]. International Journal of Systematic and Evolutionary Microbiology, 2013,63(4):1557-1561.
[27]	Wang S, Yin Y, Wang J. Microbial degradation of triclosan by a novel strain of Dyella sp.[J]. Applied Microbiology and Biotechnology, 2018,102(4):1997-2006.
[28]	Wu H, Wang M, Zhu S, et al. Structure and function of microbial community associated with phenol co-substrate in degradation of benzo[a] pyrene in coking wastewater[J]. Chemosphere, 2019,228:128-138.
[29]	Kumar M, Revathi K, Khanna S. Biodegradation of cellulosic and lignocellulosic waste by Pseudoxanthomonas sp R-28[J]. Carbohydrate Polymers, 2015,134:761-766.
[30]	Khehra M S, Saini H S, Sharma D K, et al. Biodegradation of azo dye C.I. acid red 88by an anoxic-aerobic sequential bioreactor[J]. Dyes & Pigments, 2006,70(1):1-7.
[31]	Seesuriyachan P, Takenaka S, Kuntiya A, et al. Metabolism of azo dyes by Lactobacillus casei TISTR 1500and effects of various factors on decolorization[J]. Water Research, 2007,41(5):985-992.
[32]	Bouraie M EI, Din W S EI. Biodegradation of reactive black 5by Aeromonas hydrophila strain isolated from dye-contaminated textile wastewater[J]. Sustainable Environment Research, 2016,26(5):209-216.
[33]	Hsueh C C, Chen B Y. Comparative study on reaction selectivity of azo dye decolorization by Pseudomonas luteola[J]. Journal of Hazardous Materials, 2007,141(3):842-849.
[34]	张禄艳,王竞,吕红,等.高盐条件下染料酸性橙7的生物降解特性[J]. 中国环境科学, 2009,29(6):640-645. Zhang L Y, Wang J, Lu H, et al. Biodegradation characteristics of acid orange 7under hypersaline conditions[J]. China Environmental Science, 2009,29(6):640-645.
[35]	王天杰,苏丹,李雪,等.丝瓜络固定化微生物对土壤多环芳烃吸附-降解作用[J]. 农业环境科学学报, 2020,39(1):108-117. Wang T J, Su D, Li X, et al. Adsorption-degradation of polycyclic aromatic hydrocarbons in soil by immobilized microorganisms in loofah[J]. Journal of Agro-Environment Science, 2020,39(1):108-117.