上升流速对CANON工艺稳定性及微生物群落的影响

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Abstract Expanded Granular Sludge Bed (EGSB) reactor was used as the start-up device of the CANON process in this experiment, in the effects of different upflow velocity on the nitrogen removal performance of the Completely Autotrophic Nitrogen removal Over Nitrite (CANON) process was investigated. The change of particle size of granular sludge in Integrated Fixed-film and Activated Sludge (IFAS) system and the biomass of biofilm were quantitatively analyzed. In addition, high-throughput sequencing analysis was carried out for microorganisms on granular sludge and biofilm to explore the characteristics of microbial community structure on different aggregates. The results showed that the total nitrogen removal rate (NRR) increased from 0.20kg/(m³·d) to 0.66kg/(m³·d) in the continuous operation process when the upflow velocity increased from 2m/h to 6m/h. The ratio of ΔNO₃^--N/ΔNH₄⁺-N was steadily kept at 0.11, a realization of the efficient and stable operation of CANON. When the upflow velocity increased to 8m/h, the nitrogen removal performance of CANON process was unstable, the NRR decreased to 0.42kg/(m³·d), and the average particle size of sludge decreased from 1.3mm to 0.9mm. When the upflow velocity restored back to 6m/h, the nitrogen removal performance of CANON process gradually recovered. Ultimately, the NRR was stabilized at 0.60kg/(m³·d), the average particle size of sludge was restored to 1.2mm, and the specific growth rate of biofilm biomass was 0.0024d^-1. High throughput sequencing showed that Aerobic Ammonia Oxidation Bacteria (AerAOB) functional bacteria Nitrosomonas (2.45%) and Anaerobic Ammonia Oxidation Bacteria (AnAOB) functional bacteria Candidatus kuenenia (2.38%) were the main genera in the granular sludge. The main bacteria in the biofilm were AnAOB functional bacteria Candidatus kuenenia (9.78%) and Candidatus brocadia (4.23%), while a small amount of AerAOB functional bacteria Nitrosomonas (0.40%) were also detected. The results suggested that there were some differences in two microorganisms in different aggregates.

Key words： completely autotrophic nitrogen removal over nitrite (CANON) expanded granular sludge bed (EGSB) upflow velocity nitrogen removal high-throughput sequencing

Received: 14 September 2020

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X703.5

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	ZHANG Kai
	SUN Meng-xia
	LIANG Dong-bo
	WANG Jia
	LI Jun

Cite this article:

ZHANG Kai,SUN Meng-xia,LIANG Dong-bo等. The Effect of upflow velocity on CANON process stability and microbial community[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(4): 1737-1745.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I4/1737

[1]	Wu P, Zhang X X, Wang X Z, et al. Characterization of the start-up of single and two-stage Anammox processes with real low-strength wastewater treatment[J]. Chemosphere, 2020,245:125572.
[2]	Clara R, Suarez-Ojeda E, Perez M, et al. Stable long-term operation of an upflow anammox sludge bed reactor at mainstream conditions[J]. Water Research. 2018,128:331-340.
[3]	Third K A, Sliekers A O, Kuenen J G, et al. The CANON system (completely autotrophic nitrogen-removal over nitrite) under ammonium limitation interaction and competition between three groups of bacteria[J]. Systematic and Applied Microbiology 2001, 24(4):588-596.
[4]	Yi X, Haiyuan M, Zhe K, et al. Bulking and floatation of the anammox-HAP granule caused by low phosphate concentration in the anammox reactor of expanded granular sludge bed (EGSB)[J]. Bioresource Technology, 2020,310:123421.
[5]	Yang Y D, Zhang L, Cheng J, et al. Achieve efficient nitrogen removal from real sewage in a plug-flow integrated fixed-film activated sludge (IFAS) reactor via partial nitritation/anammox pathway[J]. Bioresource Technology, 2017,239:294-301.
[6]	Malovanyy A, Trela J, Plaza E. Mainstream wastewater treatment in integrated fixed film activated sludge (IFAS) reactor by partial nitritation/anammox process[J]. Bioresource Technology, 2015,198:478-487.
[7]	Gilbert E.M, Agrawal S, Karst S M, et al. Low temperature partial nitritation/anammox in a moving bed biofilm reactor treating low strength wastewater[J]. Environmental. Science & Technology, 2014,48,8784-8792.
[8]	Hubaux N, Wells G, Morgenroth E, et al. Impact of coexistence of flocs and biofilm on performance of combined nitritation-anammox granular sludge reactors[J]. Water Research, 2015,68:127-139.
[9]	Zhang L, Liu M, Zhang S, et al. Integrated fixed-biofilm activated sludge reactor as a powerful tool to enrich anammox biofilm and granular sludge[J]. Chemosphere, 2015,140:114-118.
[10]	Malovanyy A, Yang, J, Trela, J, et al. Combination of upflow anaerobic sludge blanket (UASB) reactor and partial nitritation/anammox moving bed biofilm reactor (MBBR) for municipal wastewater treatment.[J]. Bioresource Technology, 2015,180:144-153.
[11]	Daverey A, Su S H, Huang Y T, et al. Partial nitrification and anammox process:A method for high strength optoelectronic industrial wastewater treatment.[J]. Water Research, 2013,47(9):2929-2937.
[12]	韩文杰,吴迪,周家中,等.CANON生物膜载体储存及活性恢复研究[J]. 中国环境科学, 2020,40(5):2062-2072. Han W J, Wu D, Zhou J Z, et al. Research on the storage and activity recovery of CANON suspended carrier biofilm[J]. China Environmental Science, 2020,40(5):2062-2072.
[13]	Ali M, Oshiki M, Awata T, et al. Physiological characterization of anaerobic ammonium oxidizing bacterium'Candidatus Jetteniacaeni'[J]. Environmental Microbiology, 2015,17(6):2172-2189.
[14]	国家环境保护总局.水和废水监测分析方法[M]. 第四版,北京:中国环境科学出版社, 2002. State Environmental Protection Administration of China. Water and wastewater test and analysis methods[M]. Fourth edition, Beijing:China Environmental Science Press, 2002.
[15]	Laguna A. A simple and low cost technique for determining the granulometry of upflow anaerobic sludge blanket reactor sludge[J]. Water Science Technology, 2000,40:1-8.
[16]	Bian W, Zhang S, Zhang Y, et al. Achieving nitritation in a continuous moving bed biofilm reactor at different temperatures through ratio control[J]. Bioresource Technology, 2017,226:73-79.
[17]	Perera M K, Englehardt J D, Tchobanoglous G, et al. Control of nitrification/denitrification in an onsite two-chamber intermittently aerated membrane bioreactor with alkalinity and carbon addition:Model and experiment[J]. Water Research, 2017,115:94-110.
[18]	李佳,李夕耀,张琼,等.投加羟胺原位恢复城市污水短程硝化-厌氧氨氧化工艺[J]. 中国环境科学, 2019,39(7):2789-2795. Li J, Li X Y, Zhang Q, et al. In-situ restoring demostic wastewater partial nitritation/anammox (PN/A) process by addition of hydroxylamine[J]. China Environmental Science, 2019,39(7):2789-2795.
[19]	Liu W, Yang D, Chen W, et al. High-throughput sequencing-based microbial characterization of size fractionated biomass in an anoxic anammox reactor for low-strength wastewater at low temperatures[J]. Bioresource Technology, 2017,231:45-52.
[20]	史勤,常青龙,谢弘超,等.吸附-全程自养脱氮(A-CANON)工艺处理实际市政污水[J]. 中国环境科学, 2019,39(2):598-603. Shi Q, Chang Q L, Xie H C, et al. The performance of the adsorption (A)-CANON process treatment municipal wastewater[J]. China Environmental Science, 2019,39(2):598-603.
[21]	张肖静.基于MBR的全程自养脱氮工艺(CANON)性能及微生物特性研究[D]. 哈尔滨:哈尔滨工业大学, 2014. Zhang X J Performance and microbial characteristics of completely autotrophic nitrogen removal over nitrite (CANON) in membrane bioreactor[D]. Harbin:Harbin Institute of Technology, 2014.
[22]	张铃敏,常青龙,史勤,等.CANON工艺短程硝化恢复调控及微生物种群结构变化[J]. 中国环境科学, 2019,39(6):2354-2360. Zhang L M, Chang Q L, Shi Q, et al. The recovery regulation of a CANON system and variations in the microbial community[J]. China Environmental Science, 2019,39(6):2354-2360.
[23]	刘勇,李瀚翔,邓雄文,等.氮负荷和上升流速对单级自养脱氮工艺的效能分析[J]. 工业水处理, 2017,37(12):48-52. Liu Y, Li H X, Deng X W, et al. Efficiency analysis on one-step autotrophic nitrogen removal by nitrogen loading and upflow velocity[J]. Industrial Water Treatment, 2017,37(12):48-52.
[24]	Jardin N, Hennerkes J. Full-scale experience with the deammonification process to treat high strength sludge water-a case study[J]. Water Science and Technology, 2012,65:447-455.
[25]	Yang S, Peng Y, Zhang L, et al. Autotrophic nitrogen removal in an integrated fixed-biofilm activated sludge (IFAS) reactor:Anammox bacteria enriched in the flocs have been overlooked[J]. Bioresource Technology, 2019,288:121512.
[26]	Ni B J, Hu B L, Fang F, et al. Microbial and physicochemical characteristics of compact anaerobic ammonium-oxidizing granules in an upflow anaerobic sludge blanket reactor[J]. Applied and Environmental Microbiology, 2010,76(8):2652-2656.
[27]	孙庆花,吴迪,周家中,等.CANON中试反应器启动及性能优化[J]. 环境科学, 2019,40(7):3169-3178. Sun Q H, Wu D, Zhou J Z, et al. Start-up and Performance Optimization of a CANON Pilot Reactor[J]. Environmental Science, 2019,40(7):3169-3178.
[28]	Bagchi S, Biswas R, Nandy T. Autotrophic Ammonia Removal Processes:Ecology to Technology[J]. Critical Reviews in Environmental Science and Technology, 2012,42(13):1353-1418.
[29]	Kartal B, van Niftrik L, Rattray J, et al. Candidatus ‘Brocadia fulgida’:an autofluorescent anaerobic ammonium oxidizing bacterium[J]. FEMS Microbiology Ecology, 2008,63(1):46-55.
[30]	张志强,关笑,吕锋,等.悬浮填料对厌氧氨氧化MBR运行的影响特性及机理[J]. 中国环境科学, 2018,38(3):929-934. Zhang Z Q, Guan X, Lv F, et al. Influencing characteristics and mechanisms of suspended carriers on anammox MBR performance[J]. China Environmental Science, 2018,38(3):929-934.