高效苯酚降解菌<i>Bacillus</i> sp.L5-1的分离及其降解特性

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Abstract A highly efficient phenol-degrading bacterium named L5-1 was isolated and screened from the activated sludge from a sewage treatment plant. The colony morphology observation and 16S rDNA gene sequencing showed that the strain L5-1 was Bacillus cereus, with the registration number of MN784421 in the US National Center for Biotechnology Information (NCBI). A series of experiments with Phenol as the only carbon source were conducted to study the growth and phenol degradation characteristics of this strain L5-1. The results showed that under the conditions of 10% inoculum, temperature range of 30 to 35℃, pH range of 7 to 8, the strain L5-1 effectively degraded phenol in the culture medium (with the 100mL of medium volume and the initial phenol concentration of 500mg/L), the degradation rate was better than 93% in 14h. Under optimal degradation conditions (10% inoculum, culture temperature at 35℃, pH 7.0, and NaCl concentration at 1%), The phenol degradation rate reached 97.1% within 14 hours when the initial concentration was set at 500mg/L. When the initial phenol concentration was set to 1000mg/L, the strain L5-1 still reached 97.71% degradation rate within 46 hours. The Haldane kinetic model was used to simulate the growth process of strains under different concentrations of phenol. The maximum specific growth rate was 0.355h^-1, the semi-saturation constant was 104.27mg/L, the inhibition constant was 322.83mg/L, R²=0.997. This study confirmed Strain L5-1 was a Bacillus strains with strong phenol degradation ability among the reported strains of the genus Bacillus, and provided certain theoretical references for the actual treatment of phenol-containing wastewater.

Key words： Bacillus cereus phenol biodegradable kinetics

Received: 25 September 2020

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X172

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	Articles by authors
	LIU Qing-hui
	LI Jian
	YANG Hang
	WANG Zhi-yu
	LI Yan
	ZHANG Wei-chuan
	JIA Yin-juan
	ZHANG Qiu-gen
	LUO Xu-biao

Cite this article:

LIU Qing-hui,LI Jian,YANG Hang等. Isolation and degradation characteristics of highly efficient phenol-degrading bacteria Bacillus sp. L5-1[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(5): 2441-2448.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I5/2441

[1]	Mao Z, Yu C, Xin L. Enhancement of phenol biodegradation by Pseudochrobactrum sp. through ultraviolet-induced mutation[J]. International Journal of Molecular Sciences. 2015,16(12):7320-7333.
[2]	Massalha N, Shaviv A, Sabbah I. Modeling the effect of immobilization of microorganisms on the rate of biodegradation of phenol under inhibitory conditions[J]. Water Research, 2010,44(18):5252-5259.
[3]	王兵,刘璞真,任宏洋,等.非均相催化臭氧化降解水中苯酚动力学[J]. 环境工程学报, 2016,10(7):3427-3433. Wang B, Liu P Z, Ren H Y, et al. Degradation kinetics of catalytic ozone oxidation of phenol in water[J]. Chinese Journal of Environmental Engineering. 2016,10(7):3427-3433.
[4]	陈治希,刘昭文,杨凯,等.微生物降解酚类污染物的研究进展[J]. 广州化学, 2015,40(1):72-78. Chen Z X, Liu Z W, Yang K, et al. Progress of phenolic compounds degradation by microbes[J].Guangzhou Chemistry, 2015,40(1):72-78.
[5]	Lu Z, Guo X, Li H, et al. High-throughput screening for a moderately halophilic phenol-degrading strain and its salt tolerance response[J]. International Journal of Molecular Sciences. 2015,16(12):11834-11848.
[6]	Wei G H, Yu J F, Zhu Y H, et al. Characterization of phenol degradation by Rhizobium sp. CCNWTB 701isolated from Astragalus chrysopteru in mining tailing region[J]. Journal of Hazardous Materials, 2008,151(1):111-117.
[7]	陈明,张维,徐玉泉,等.醋酸钙不动杆菌PHEA-2对苯酚的降解特性研究[J]. 中国环境科学, 2001,21(3):226-229. Chen M, Zhang W, Xu Y Q, et al. Study on characteristics of Acinetobater calcoaceticus PHEA-2 for phenol degradation[J]. China Environmental Science, 2001,21(3):226-229.
[8]	Ahmad S A, Shamaan N A, Arif N M, et al. Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1[J]. World Journal of Microbiology and Biotechnology, 2012,28(1):347-352.
[9]	刘艳霞.降酚菌的定向驯化及其对含酚废水的降解作用[D]. 北京:北京化工大学, 2011. Liu Y X. The teaming of phenol-degraded bacteria and its biodegradability to phenolic wasterwater[D]. Beijing:Beijing University of Chemical Technology, 2011.
[10]	魏霞,周俊利,谢柳,等.苯酚降解菌CM-HZX1菌株的分离、鉴定及降解性能研究[J]. 环境科学学报, 2016,36(9):3193-3199. Wei X, Zhou J L, Xie L, et al. Isolation, identification and characterization of phenol-degrading strain CM-HZX1[J]. Acta Scientiae Circumstantiae. 2016,36(9):3193-3199.
[11]	徐庆.苯系物降解菌的筛选及其降解特性研究[D]. 曲阜:曲阜师范大学, 2017. Xu Q, Screening of benzene series degradation bacteria and study on their degradation characteristics[D]. Qufu:Qufu Normal University.
[12]	Jiang Y, Yang K, Wang H, et al. Characteristics of phenol degradation in saline conditions of a halophilic strain JS3 isolated from industrial activated sludge[J]. Marine Pollution Bulletin, 2015,99(1/2):230-234.
[13]	陈晓华,魏刚,刘思远,等.高效降酚菌株Ochrobactrum sp. CH10生长动力学和苯酚降解特性的研究[J]. 环境科学, 2012,33(11):3956-3961. Xu X H, Wei G, Liu S Y, et al. Growth kinetics and phenol degradation of highly efficient phenol-degrading Ochrobactrum sp. CH10[J]. Environmental Science, 2012,33(11):3956-3961.
[14]	王图锦,潘瑾,刘雪莲.高效苯酚降解菌PDB1的筛选及降解特性研究[J]. 科学技术与工程, 2017,17(2):301-304. Wang T J, Pan J, Liu X L, et al. Breeding of Phenol-degradation Bacteria and Studyon Phenol Biodegradation by the Strain PDB1[J]. Science Technology and Engineering, 2017,17(2):301-304.
[15]	Shourian M, Noghabi K A, Zahiri H S, et al. Efficient phenol degradation by a newly characterized Pseudomonas sp. SA01 isolated from pharmaceutical wastewaters[J]. Desalination, 2009,246(1-3):577-594.
[16]	Ereqat S I, Abdelkader A A, Nasereddin A F, et al. Isolation and characterization of phenol degrading bacterium strain Bacillus thuringiensis J20 from olive waste in Palestine[J]. Journal of Environmental Science and Health, Part A. 2018,53(1):39-45.
[17]	Arutchelvan V, Kanakasabai V, Elangovan R, et al. Kinetics of high strength phenol degradation using Bacillus brevis [J]. Journal of Hazardous Materials. 2006,129(1-3):216-222.
[18]	刘鸿杰,何熙璞,李浩,等.苯酚降解菌F6的筛选鉴定及降解特性[J]. 基因组学与应用生物学, 2017,36(1):233-238. Liu H J, He X P, Li H, et al. Isolation and identification of phenol degradation strain F6 and its degradation characteristic[J]. Genomics and Applied Biology, 2017,36(1):233-238.
[19]	于彩虹,陈飞,胡琳娜,等.一株苯酚降解菌的筛选及降解动力学特性[J]. 环境工程学报, 2014,8(3):1215-1220. Yu C H, Chen F, Hu L N, et al. Selection of phenol degradation bacteria and characteristic of degradation kinetics[J]. Chinese Journal of Environmental Engineering, 2014,8(3):1215-1220.
[20]	HJ 503-2009水质水中挥发酚类测定4-氨基安替比林分光光度法[S]. HJ 503-2009 Water quality-ddetrmination of volatile phenolic compounds-4-AAP spectrophotometric method[S].
[21]	冯玉雪,毛缜,吕蒙蒙.一株DDT降解菌的筛选及其降解特性[J]. 中国环境科学, 2018,38(5):1935-1942. Feng Y X, Mao Z, Lv M M. Screening and degradation characteristics of a DDT-degrading bacteria[J]. China Environmental Science, 2018,38(5):1935-1942.
[22]	葛启隆,王国英,岳秀萍.波茨坦短芽孢杆菌降解苯酚特性及动力学研究[J]. 生物技术通报, 2014,(3):117-122. Ge Q L, Wang G Y, Yue X P. Phenol degradation by Brevibacillus borstelensis and kinetic analysis[J]. Biotechnology Bulletin, 2014, (3):117-122.
[23]	赵娜娜,许继飞,宋晓雪,等.嗜盐高效降酚菌株Halomonas sp. H17的筛选及降解苯酚特性[J]. 环境科学学报, 2019,39(2):318-324. Zhao N N, Xu J F, Song X X, et al. Screening and phenol-degrading characteristics of a highly efficient phenoldegrading halophilic bacterial strain Halomonas sp.H17[J]. Acta Scientiae Circumstantiae, 2019,39(2):318-324.
[24]	Levén L, Nyberg K, Schnürer A. Conversion of phenols during anaerobic digestion of organic solid waste-A review of important microorganisms and impact of temperature[J]. Journal of Environmental Management, 2012,95(1):S99-S103.
[25]	Kuang Y, Zhou Y, Chen Z L, et al. Impact of Fe and Ni/Fe nanoparticles on biodegradation of phenol by the strain Bacillus fusiformis (BFN) at various pH values[J]. Bioresource Technology, 2013,136(Complete):588-594.
[26]	张安龙,王晔,王雪青,等.一株高效苯酚降解真菌的分离鉴定及其菌剂的制备[J]. 微生物学通报, 2018,45(7):1450-1461. Zhang A L, Wang Y, Wang X Q, et al. Isolation and identification of a high-efficiency phenol-degrading fungi and the preparation of its microbial inoculum[J]. Microbiology China, 2018,45(7):1450-1461.
[27]	张立国,刘建忠,班巧英,等.弱酸性条件下丙酸富集培养物的降解特性[J]. 中国环境科学, 2016,36(12):3724-3728. Zhang L G, Liu J Z, Ban Q Y, et al. Degradation characteristics of a propionate enriched culture at slightly acidic conditions[J]. China Environmental Science, 2016,36(12):3724-3728.
[28]	Li H, Meng F, Duan W, et al. Biodegradation of phenol in saline or hypersaline environments by bacteria:A review[J]. Ecotoxicology and Environmental Safety, 2019,184:109658.
[29]	王丽娟,钱子雯,沈海波,等.一株耐盐菌的分离及其降解特性[J]. 化工进展, 2017,36(3):1047-1051. Wang L J, Qian Z W, Shen H B, et al. Separation and biodegradation characteristics of a halotolerant strain[J]. Chemical Industry and Engineering Progress, 2017,36(3):1047-1051.
[30]	黄中子,李辉,刘勇弟,等.一株中度嗜盐菌Virgibacillu sp.PDB-F2对苯酚的降解特性[J]. 环境科学与技术, 2015,38(12):1-5. Huang Z Z, Li H, Liu Y D, et al. Characteristics of phenol biodegradation by a moderately halophilic bacterium Virgibacillu sp. PDB-F2[J]. Environmental Science & Technology, 2015,38(12):1-5.
[31]	马溪平,艾娇,徐成斌,等.耐低温苯酚降解菌的降解动力学研究[J]. 环境保护科学, 2009,35(5):18-21. Ma X P, Ai J, Xu C B, et al. Study on kinetics of phenol biodegradation by low temperature-resistance strain[J]. Environmental Protection Science, 2009,35(5):18-21.
[32]	Jiang Y, Wen J, Bai J, et al. Biodegradation of phenol at high initial concentration by Alcaligenes faecalis [J]. Journal of Hazardous Materials, 2007,147(1/2):672-676.