倏逝波光纤传感器快速检测诺氟沙星

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Abstract Norfloxacin (NOR), a widely used antibiotic, is becoming a major threat to ecological security. The existing detection methods have either high cost and pretreatment requirements or long detection time and poor sensitivity. Based on indirect competitive immunoassay principle, a new detection method of norfloxacin was developed using the evanescent wave fiber optic bio sensing platform. The rapid and sensitive detection of norfloxacin in water was achieved. The optimal detection conditions of norfloxacin were as follows: antibody concentration was 1μg/mL, pre-reaction time was 1min, and reaction time was 4min. Under the optimal conditions, the detection limit of norfloxacin was 1.89μg/L. The coating-antigen modified fiber optic probe showed good specificity for fluorescence-labeled anti-norfloxacin antibody and stability, and could be reused more than 400 times. The recovery results of the spiked water samples, such as tap water, landscape water, and effluent of secondary sedimentation tank, demonstrated that the proposed biosensor had good precision and accuracy, and was less affected by the environmental matrix. The presented biosensor can be used for the rapid detection of NOR in actual water samples.

Key words： norfloxacin antibiotics evanescent wave biosensor immunoassay environmental monitoring

Received: 22 September 2021

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X83

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	Articles by authors
	ZHUO Yu-xin
	XU Wen-juan
	CHENG Yuan
	SONG Dan
	HAN Xiang-zhi
	LONG Feng

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ZHUO Yu-xin,XU Wen-juan,CHENG Yuan等. Rapid detection of norfloxacin in water by evanescent wave fiber optic biosensor[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(5): 2283-2288.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I5/2283

[1]	Carvalho I T, Santos L. Antibiotics in the aquatic environments: A review of the European scenario [J]. Environment International, 2016,94:736-757.
[2]	Zhang Q, Ying G, Pan C, et al. Comprehensive evaluation of antibiotics emission and fate in the river basins of China: source analysis, multimedia modeling, and linkage to bacterial resistance [J]. Environmental science & technology, 2015,49(11):6772-6782.
[3]	周志洪,吴清柱,王秀娟,等.环境中抗生素污染现状及检测技术 [J]. 分析仪器, 2016,(6):1-8. Zhou Z, Wu Q, Wang X, et al. Present situation and detection technology of antibiotic pollution in environment [J]. Analysis instrument, 2016,(6):1-8.
[4]	Liu Y, Wang Y, Walsh T R, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1in animals and human beings in China: a microbiological and molecular biological study [J]. The Lancet Infectious Diseases, 2016,16(2):161-168.
[5]	毛智琼,金永才,刘璇,等.我国与欧盟、美国、国际食品法典委员会的禽蛋抗生素类兽药残留限量标准对比分析 [J]. 食品安全质量检测学报, 2020,11(12):4034-4040. Mao Z, Jin Y, Liu X, et al. Comparative analysis of the limit standards of antibiotics and veterinary drug residues in poultry and eggs between my country and the European Union, the United States and the International Codex Alimentarius Commission [J]. Journal of Food Safety and Quality Inspection, 2020,11(12):4034-4040.
[6]	Cesar A, Arias B, Murray E. Antibiotic-Resistant bugs in the 21st century — A clinical super-challenge [J]. New England Journal of Medicine, 2009,360(5).
[7]	Wang H, Wang N, Wang B, et al. Antibiotics detected in urines and adipogenesis in school children [J]. Environment International, 2016,89-90:204-211.
[8]	章强,辛琦,朱静敏,等.中国主要水域抗生素污染现状及其生态环境效应研究进展 [J]. 环境化学, 2014,33(7):1075-1083. Zhang Q, Xin Q, Zhu J, et al. Research progress on the status quo of antibiotic pollution in China's main waters and its ecological and environmental effects [J]. Environmental Chemistry, 2014,33(7): 1075-1083.
[9]	杨雯筌,张晓燕,姚倩,等.液相色谱-串联质谱法检测雄蜂蛹粉中50种抗生素残留 [J]. 色谱, 2019,37(1):46-53. Wenquan Y, Xiaoyan Z, Qian Y, et al. Determination of 50 antibiotic residues in drone pupa powder by liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2019, 37(1):46-53.
[10]	王承平,秦宇,侯蓓蓓,等.UPLC-MS/MS检测生鲜牛奶中72种抗生素残留 [J]. 食品与机械, 2019,35(9):74-76. Chengping W, Yu Q, Beibei H, et al. Detection of 72 antibiotic residues in raw milk by UPLC-MS/MS [J]. Food and Machinery, 2019,35(9):74-76.
[11]	沈翠香.微生物抑制法检测动物性食品中喹诺酮类药物残留的研究 [D]. 乌鲁木齐:新疆农业大学, 2010. Shen C. Study on the detection of quinolones residues in animal food by microbial inhibition method [D]. Urumqi: Xinjiang Agricultural University, 2010.
[12]	Liang X, Li C, Zhu J, et al. Dihydropteroate synthase based sensor for screening multi-sulfonamides residue and its comparison with broad-specific antibody based immunoassay by molecular modeling analysis [J]. Analytica Chimica Acta, 2019,1050:139-145.
[13]	韩璐,黄大波.生物传感器在环境污染物检测中的应用 [J]. 山东化工, 2017,46(12):84-85. Han L, Huang D. Application of biosensor in environmental pollutant detection [J]. Shandong Chemical, 2017,46(12):84-85.
[14]	Hoseinian M, Ahmadi A, Bezgabadi A, et al. Simulation of wagon wheel optical fiber biosensor for quick and easy detection of cancer cells [J]. Optical and Quantum Electronics, 2021,53.
[15]	龙峰,施汉昌,何苗,等.倏逝波荧光免疫传感器在环境检测中的研究进展 [J]. 环境科学, 2008,(3):545-550. Long F, Shi H, He M, et al. Research progress of evanescent wave fluorescence immunosensors in environmental detection [J]. Environmental Science, 2008,(3):545-550.
[16]	李伟,吴君,王浩宇,等.倏逝波免疫传感器超灵敏检测水样中的双酚A [J]. 环境化学, 2018,37(2):347-352. Wei L I, Jun W, Haoyu W, et al. Ultrasensitive detection of BPA in water using an evanescent wave immunosensor [J]. Environmental Chemistry, 2018,37(2):347-352.
[17]	Cheng Y, Wang H, Zhuo Y, et al. Reusable smartphone-facilitated mobile fluorescence biosensor for rapid and sensitive on-site quantitative detection of trace pollutants [J]. Biosensors and Bioelectronics, 2022,199:113863.
[18]	Xu W, Liu J, Song D, et al. Rapid, label-free, and sensitive point- of-care testing of anti-SARS-CoV-2IgM/IgG using all-fiber Fresnel reflection microfluidic biosensor [J]. Microchimica Acta, 2021,188(8).
[19]	Long F, Gao C, Shi H C, et al. Reusable evanescent wave DNA biosensor for rapid, highly sensitive, and selective detection of mercury ions [J]. Biosensors and Bioelectronics, 2011,26(10):4018- 4023.
[20]	Long F, Shi H C, He M, et al. Sensitive and rapid detection of 2,4-dicholorophenoxyacetic acid in water samples by using evanescent wave all-fiber immunosensor [J]. Biosensors and Bioelectronics, 2008,23(9):1361-1366.
[21]	龙峰,施汉昌,王洪臣,等.用于1,3-二硝基苯快速检测的免疫传感器研究 [J]. 环境科学, 2014,(4):1561-1565. Long F, Shi H, Wang H, et al. Study on immunosensor for rapid detection of 1,3-dinitrobenzene [J]. Environmental Science, 2014,(4): 1561-1565.
[22]	许振成.环境痕量污染物及其防控对策 [J]. 环境科学与管理, 2009,34(11):39-42. Xu Z. Environmental trace pollutants and their prevention and control countermeasures [J]. Environmental Science and Management, 2009, 34(11):39-42.
[23]	Midgley A J, Niswender G D, Rebar R W. Principles for the assessment of the reliability of radioimmunoassay methods (precision, accuracy, sensitivity, specificity) [J]. Acta Endocrinol. Suppl. (Copenh), 1969,142:163-184.
[24]	谢文佳,孙晓霞,王前,等.基于固相萃取-高效液相色谱-串联质谱法同时测定饮用水中14种抗生素 [J]. 轻工标准与质量, 2021,(5):6. Xie W, Sun X, Wang Q, et al. Simultaneous determination of 14antibiotics in drinking water based on solid phase extraction-high performance liquid chromatography-tandem mass spectrometry [J]. Light Industry Standard and Quality, 2021,(5):6.
[25]	李树莹.水中喹诺酮类抗生素免疫检测技术研究 [D]. 北京:清华大学, 2018. Li S. Study on immunodetection technology of quinolone antibiotics in water [D]. Beijing: Tsinghua University, 2018.