氧化硫硫杆菌固定化菌球制备及其耦合填料去除H<sub>2</sub>S

Abstract
Figure/Table
References
Related Citation (12)

Download: PDF (0 KB) HTML (0 KB)
Export: BibTeX | EndNote (RIS)

Abstract Acidithiobacillus thiooxidans was immobilized to prepare bacterial pellet using sodium alginate (SA) as the carrier. The microbial immobilization process was optimized and the removal capacity of the bacterial pellet to H₂S was examined in a biofilter. Meanwhile, acclimated activated sludge containing sulfur malodorous eliminating microbial consortium obtained previously was employed as bacterial source to screen packing media in biofilter. Lastly, A. thiooxidans immobilized pellet and the screened packing media were coupled to biotrickling filtration deodorization. The results showed that the optimal conditions for microbial immobilization process were 3.0% of SA, adding adsorbent carbon nanotubes (CNT) into SA solution, keeping the ratio of A. thiooxidans bacterial suspension solution to the mixture solution at 20%, 4.0% of CaCl₂, suspending immobilized pellet into hexanediamine (HDA) solution for surface modification. Filling A. thiooxidans immobilized pellet into biofilter to eliminate H₂S, and the removal efficiency and elimination capacity of H₂S reached 70% and 1.06g H₂S/m³·h. Activated carbon cloth was screened as the best packing media among polyurethane foam, activated carbon cloth and ceramsite, and the removal efficiency and elimination capacity of H₂S reached 88% and 0.84g H₂S/m³·h in this case. Filling A. thiooxidans immobilized pellet and activated carbon cloth into biofilter with mixing pattern to eliminate H₂S, the removal efficiency and elimination capacity of H₂S reached 86% and 1.00g H₂S/m³·h.

Key words： microbial immobilization Acidithiobacillus thiooxidans hydrogen sulfide biotrickling filtration

Received: 27 October 2021

PACS:	X172
	X701

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	SHI Chao-hong
	LU Yu-sheng
	GU Wen-jie
	XIE Kai-zhi
	XU Pei-zhi
	WANG Dan
	PENG Huan-long
	LI Ya-ying
	SUN Li-li

Cite this article:

SHI Chao-hong,LU Yu-sheng,GU Wen-jie等. The preparation of Acidithiobacillus thiooxidans immobilized pellet and its coupling removal of H₂S with packing material[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(5): 2331-2338.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I5/2331

[1]	Lee M, Wi J, Koziel J A, et al. Effects of UV-A light treatment on ammonia, hydrogen sulfide, greenhouse gases, and ozone in simulated poultry barn conditions [J]. Atmosphere, 2020,11:283.
[2]	Jia H H, Gao S, Duan Y S, et al. Investigation of health risk assessment and odor pollution of volatile organic compounds from industrial activities in the Yangtze River Delta region, China [J]. Ecotoxicology and Environmental Safety, 2021,208:111474.
[3]	Marquez P, Benitez A, Caballero A, et al. Integral evaluation of granular activated carbon at four stages of a full-scale WWTP deodorization system [J]. Science of the Total Environment, 2021, 754:142237.
[4]	Vikrant K, Kailasa S K, Kailasa D C W, et al. Biofiltrationn of hydrogen sulfide: trends and challenges [J]. Journal of Cleaner Production, 2018,187:131-147.
[5]	赵茹涵,杨庆,彭赵旭,等.污水处理厂挥发性硫化物释放特征与风险评价 [J]. 中国环境科学, 2021,41:5570-5577. Zhao R H, Yang Q, Peng Z X, et al. Release characteristics and risk evaluation of VSCs in municipal wastewater treatment plants [J]. China Environmental Science, 2021,41:5570-5577.
[6]	Khoshnevisan B, Tsapekos P, Alfaro N, et al. A review on prospects and challenges of biological H2S removal from biogas with focus on biotrickling filtration and microaerobic desulfurization [J]. Biofuel Research Journal, 2017,16:741-750.
[7]	Wu H, Guo C, Yin Z, et al. Performance and bacterial diversity of biotrickling filters filled with conductive packing material for the treatment of toluene [J]. Bioresource Technology, 2018,257:201-209.
[8]	Wysocka I, Gębicki J, Namieśnik J. Technologies for deodorization of malodorous gases [J]. Environmental Science and Pollution Research, 2019,26:9409-9434.
[9]	Solcia R B, Ramíreza M, Fernándeza M, et al. Hydrogen sulfide removal from air by biotrickling filter using open-pore polyurethane foam as a carrier [J]. Biochemical Engineering Journal, 2014,84:1-8.
[10]	赵永顺,柳洋,陈正军.氧化硫硫杆菌在淀粉糖废水原位除臭应用初探 [J]. 净水技术, 2021,40:381-385. Zhao Y S, Liu Y, Chen ZJ. Application of Thiobacillus thiooxidans in-situ deodorizing in starch sugar wastewater: A preliminary study [J]. Water Purification Technology, 2021,40:381-385.
[11]	Cha J M, Shin H J, Roh S H, et al. Hydrogen sulfide removal by immobilized Thiobacillus novellas on SiO2 in a fluidized bed reactor [J]. Journal of Microbiology and Biotechnology, 2007,17:320-324.
[12]	Chung Y C, Huang C P, Tseng C P. Removal of hydrogen sulphide by immobilized Thiobacillus sp. strain CH11in a biofilter [J]. Journal of Chemical Technology and Biotechnology, 1997,69:58-62.
[13]	Guo J Y, Chen C, Chen W J, et al. Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater [J]. Chemosphere, 2021,284:131266-131266.
[14]	Mehrotra T, Dev S, Banerjee A, et al. Use of immobilized bacteria for environmental bioremediation: a review [J]. Journal of Environmental Chemical Engineering, 2021,9:105920.
[15]	Liu C, Liu J, Li J, et al. Removal of H2S by co-immobilized bacteria and fungi biocatalysts in a bio-trickling filter [J]. Process Safety and Environmental Protection, 2013,91:145-152.
[16]	许海朋,李岩,牧辉,等.固定化排硫硫杆菌对气体中H2S去除特性 [J]. 环境工程学报, 2018,11:3109-3115. Xu H P, Li Y, Mu H, et al. Removal of H2S by Tiobacillus thioparus immobilized on different matrices [J]. Chinese Journal of Environmental Engineering, 2018,12:3109-3115.
[17]	宋永伟,王蕊,杨琳琳,等.三种次生矿物固定A.ferrooxidans的Fe2+氧化及成矿性能比较 [J]. 中国环境科学, 2020,40:2073-2080. Song Y W, Wang R, Yang L L, et al. Fe2+ oxidation and mineralization properties of Acidithiobacillus ferrooxidans immobilized on three secondary iron minerals [J]. China Environmental Science, 2020,40: 2073-2080.
[18]	王玉建,涂玮,杨晓娟,等.固定化氧化亚铁硫杆菌处理硫化氢新工艺研究 [J]. 化学工程, 2007,11:40-42. Wang Y J, Tu W, Yang X J, et al. New technology of hydrogen sulfide removal by immobilized Acidithiobacillus ferrooxidans [J]. Chemical Engineering (China), 2007,11:40-42.
[19]	陈诚,郭俊元,周明杰,等.壳聚糖-海藻酸钠固定化菌小球处理猪场沼液 [J]. 中国环境科学, 2019,39:2812-2821. Chen C, Guo J Y, Zhou M J, et al. Removal of ammonium from aqueous solution by microorganism cells immobilized into chitosan- sodium alginate beads [J]. China Environmental Science, 2019,39: 2812-2821.
[20]	苏有升.生物滴滤法处理制药厂恶臭及VOCs的运行实践研究 [D]. 杭州:浙江工业大学, 2020. Su Y S. Operation practice of biotrickling filter treatment for malodor and VOCs in pharmaceutical factory [D]. Hangzhou: Zhejiang University of Technology, 2020.
[21]	Aita B C, Mayer F D, Muratt D T, et al. Biofiltration of H2S-rich biogas using Acidithiobacillus thiooxidans [J]. Clean technologies and environmental policy, 2016,18:689-703.
[22]	Tsang Y F, Wang L, Chua H. Simultaneous hydrogen sulfide and ammonia removal in a biotrickling filter: crossed inhibitory effects among selected pollutants and microbial community change [J]. Chemical Engineering Journal, 2015,281:389-396.
[23]	刘建伟,吕臣,夏雪峰,等.生物滤池处理城市污水工艺中恶臭和微生物气溶胶的填料选择 [J]. 环境工程学报, 2014,11:273-279. Liu J W, Lu C, Xia X, et al. Selection of packing materials for biofilter to treat odor and bioaerosol from municipal wastewater treatment process [J]. Chinese Journal of Environmental Engineering, 2014,11: 273-279.
[24]	陈思茹.生物炭填料净化恶臭气体的应用研究 [D]. 广州:华南理工大学, 2019. Chen S R. Study on the application of biochar filler for odorous gas purification [D]. Guangzhou: South China University of Technology, 2019.
[25]	庄荣玉,王磊刚,李美燕,等.异养菌与新型填料成膜性及BTF处理屠宰H2S废气 [J]. 中国环境科学, 2020,40:1106-1115. Zhuang R Y, Wang L G, Li M Y, et al. Biofilm formation of a heterotrophic bacterium on the novel packing material and the removal of hydrogen sulfide produced in a slaughtering process by biotrickling filter [J]. China Environmental Science, 2020,40:1106-1115.
[26]	孙霞,刘扬,张虎,等.聚乙烯醇-海藻酸钠-改性沸石固定化菌球降解氨氮的研究 [J]. 化学与生物工程, 2021,9:32-36. Sun X, Liu Y, Zhang H, et al. Removal of Ammonia nitrogen by polyvinyl alcohol-sodium alginate-modified zoelite immobilized bacteria pellets [J]. Chemistry & Bioengineering, 2021,9:32-36.
[27]	陈曦.多环芳烃降解菌包埋固定化及其降解特性研究 [D]. 重庆:重庆大学, 2018. Chen X. Research on immobilization of polycyclic aromatic hydrocarbons degrading bacteria and the degradation characteristics of PAHs by immobilization strains [D]. Chongqing: Chongqing University, 2018.
[28]	戴云飞,杨泽玉,陈颖,等.聚乙烯醇- 海藻酸钠-活性炭固定化菌球处理二氯甲烷的研究 [J]. 环境科学学报, 2021,41:430-439. Dai Y F, Yang Z Y, Chen Y, et al. Removal of DCM by microorganism cells immobilized into polyvinyl alcohol-alginate-activated carbon beads [J]. Acta Scientiae Circumstantiae, 2021,41:430-439.
[29]	Wang B, Xu X Y, Yao X W, et al. Degradation of phenanthrene and fluoranthene in a slurry bioreactor using free and Ca-alginate immobilized Sphingomonas pseudosanguinis and Pseudomonas stutzeri bacteria [J]. Journal of Environmental Management, 2019,249:109388.
[30]	Xue J L, Wu Y N, Shi K, et al. Study on the degradation performance and kinetics of immobilized cells in straw-alginate beads in marine environment [J]. Bioresource Technology, 2019,280:88-94.
[31]	Dong Y W, Zhang Y Q, Tu B J, et al. Immobilization of ammonia- oxidizing bacteria by calcium alginate [J]. Ecological Engineering, 2014,73:809-814.
[32]	杜青平,陈展明,李彦旭,等.活性炭含量对PVA-SA固定化小球处理氯苯微污染废水的影响 [J]. 广东工业大学学报, 2017,34:22-26. Du Q P, Chen Z M, Li Y X, et al. Effects of activated carbon in sodium alginate and polyvinylacohol immobilization pellets of Penicillium sp. on chlorobenzene removal [J]. Journal of Guangdong University of Technology, 2017,34:22-26.
[33]	Nwankwegu A S, Onwosi C O. Microbial cell immobilization: a renaissance to bioaugmentation inadequacies: a review [J]. Environmental Technology Reviews, 2017,6:186-198.
[34]	Santos-Clotas E, Cabrera-Codony A, Boada E, et al. Efficient removal of siloxanes and volatile organic compounds from sewage biogas by an anoxic biotrickling filter supplemented with activated carbon [J]. Bioresource Technology, 2019,294:122136.