H<sub>2</sub>S信号在拟南芥响应SO<sub>2</sub>胁迫过程中的作用

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Abstract Sulfur dioxide (SO₂) is one of the most common and harmful air pollutant. Hydrogen sulfide (H₂S), as a new gasotransmitter, is involved in the regulation of plant development and stress adaptation. To reveal the role of H₂S in response to SO₂ stress, the relation between transcriptional response to H₂S and SO₂ treatments, and the production and physiological effect of H₂S during SO₂ fumigation will be studied in Arabidopsis plants. By using high-throughput sequencing, many genes were identified to be differentially expressed in Arabidopsis plants exposed to 30mg/m³ SO₂ or treated with 0.1mmol/L H₂S. Among these differentially expressed genes (DEGs), 122, 0genes were overlapped between SO₂ and H₂S treatments, including some genes encoding enzymes involved in sulfur and glutathione metabolism. These results suggested a interaction between H₂S and SO₂ in regulating sulfur metabolism pathways in plants. Moreover, two H₂S-generating genes, L-cysteine desulfhydrase (LCD) and L-cysteinedesulfhydrase1 (DES1) were significantly up-regulated, and endogenous H₂S content was enhanced in Arabidopsis plants under SO₂ stress. Meanwhile, the activities of superoxide dismutase (SOD) and catalase (CAT), the content of glutathione (GSH) and activities of GSH-related enzymes including glutathione S-transferase (GST) and glutathione peroxidase (GPX) were significantly increased in Arabidopsis plants exposed to SO₂. And that H₂O₂ content was markedly increased in SO₂-treated Arabidopsis plants, while malondialdehyde (MDA) content showed no significant difference compared with the control group. Exogenous application of H₂S further increased the level of GSH and GSH-related defense enzyme, and reduced the content of H₂O₂ in Arabidopsis plants under SO₂ stress. When scavenged endogenous H₂S by spraying hypotaurine (HT) during SO₂ fumigation, the content of GSH, the activities of SOD, CAT and GST were reduced, and the content of MDA was significantly increased in Arabidopsis plants. Together, this study indicated that H₂S played an important role in response to SO₂ stress through improving antioxidant capacity, which would be helpful for better understanding the adaptation mechanism of plants to environmental stress.

Key words： sulfur dioxide hydrogen sulfide Arabidopsis sulfur compounds antioxidant enzymes

Received: 10 November 2021

PACS:

X171.5

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	Articles by authors
	LI Li-hong
	GUO Yu-ru
	HOU Jun-xin
	WU Li-hua

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LI Li-hong,GUO Yu-ru,HOU Jun-xin等. Functions of H₂S signal in response to SO₂ stress in Arabidopsis thaliana[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(6): 2904-2910.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I6/2904

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