代森锰及其代谢物的神经毒性影响与机制研究

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Abstract The toxicological effects and mechanisms of Maneb and its metabolites on dopaminergic cells were investigated in depth using SH-SY5Y cells and PC12 cells as experimental samples. The results showed that the organic and metal ion components of Maneb did not have obvious toxic effects when exposed alone, but had synergistic effects when exposed in combination, and the degree of inhibition to cells viability was comparable to that of Maneb at the same concentration level. The reasons for this were related to the generation of reactive oxygen species (ROS) and apoptosis. In addition, the Western Blotting revealed that Maneb reduced the expression of Bcl-2, elevated the level of Bax and Cytochrome C and activated Caspase-3 in a dose-dependent manner, suggesting an important role of the mitochondrial apoptosis pathway in dopaminergic cells apoptosis induced by Maneb.

Key words： Maneb SH-SY5Y cells PC12 cells oxidative stress cell apoptosis

Received: 18 February 2022

PACS:

X171.5

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	Articles by authors
	LIU Chao-yang
	LIU Ze-hua
	FANG Yan-yan
	YANG Bo-yuan

Cite this article:

LIU Chao-yang,LIU Ze-hua,FANG Yan-yan等. Neurotoxic effects and mechanisms of Maneb and its metabolites[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(9): 4399-4408.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I9/4399

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