基于Illumina RNA-Seq分析的三种内分泌干扰物对斑马鱼神经毒性效应与机制

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Abstract Triclosan (TCS), triclocarban (TCC), and bispenol A (BPA) are all proved to possess endocrine disrupting effects; however, little data are available on their neurotoxicity effects on zebrafish (Danio rerio) and the underlying molecular mechanisms. Herein, zebrafish transcriptomic data was obtained with the aid of RNA-Seq after exposure to the aforementioned three pollutants. By means of bioinformatics analysis, nine common positively differential expression genes (DEGs) were screened in the three exposure treatments. The GO functions and KEGG pathways of nine DEGs were mainly attributable to biological process, cellular component, and molecular function, in which the number and function were different with varying chemical exposure. Notably, most of the DEGs were found to be enriched in the nerve-related pathways, but their detailed pathways were different for three chemicals. The computation of node-correlation degree of PPI network showed that the hub genes enriched in neural pathways were different in the three treatments, which had rich and indirect interaction networks with neural marker genes. By integrating DEGs with GBM mutant gene of glioblastoma in the TCGA database, we confirmed that chemical exposure induced GBM risk, but the related occurrence pathway and regulatory signaling pathway were different. Therefore, the underlying neurotoxicity mechanisms induced by the three pollutants were disclosed at the molecular level.

Key words： endocrine disruptors zebrafish transcriptome sequencing neurotoxicity glioblastoma

Received: 04 July 2022

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X171

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	GUO Zi-yi
	WANG Wei-wei
	SONG Jie
	WANG Hui-li

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GUO Zi-yi,WANG Wei-wei,SONG Jie等. Neurotoxicity effects of the three endocrine disruptors on zebrafish and the underlying molecular mechanisms by using the Illumina RNA-seq technique[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 946-956.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I2/946

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